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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2071 [new locus tag: NWMN_RS12000 ]
  • pan locus tag?: SAUPAN005559000
  • symbol: NWMN_2071
  • pan gene symbol?:
  • synonym:
  • product: hemolysin III

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2071 [new locus tag: NWMN_RS12000 ]
  • symbol: NWMN_2071
  • product: hemolysin III
  • replicon: chromosome
  • strand: -
  • coordinates: 2307614..2308300
  • length: 687
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGATGTCCAAATCAACTAAAAAAAGTAATGACACAATCGTAGAAACATTTAAGGATATC
    ATCCCTTTATCATTTGGAGAAGAAATTGGTAATGCGGCATCTCATGGTGTTGCAGCTTTG
    TTAACATTATTAGTGTTACCCTATGCGGCTGTGCACAGTTATATTGCACATGGTACGCTT
    GCGTCTGTGAGTATGTCTATTTATGTTATTTCTATCTTTATGATGTTTATTTCATCTACT
    ATTTATCATTCTATGCAAAATGAAACACCTCATAAATATATTTTAAGGATTATCGATCAC
    AGTATGATTTACGTAGCAATTTCTGGTACATATACGCCTATTTTGTTAACAGTTGTAGGT
    GGTTGGATAGGTTGGACAGTATTTATTTTATTATGGGGAACGACACTTTGGGGGATTTTA
    TATAAATCCATAGCTGTCAAAGTTAACCAAAAGTTAAGCTTAATTGTCTATTTAGTGATG
    GGCTGGGTAGGTATTATCTTGTTACCAATTATTTTTATTCGCACATCATGGATATTCATA
    TTATTCATTTTCTTAGGTGGTGTGTCTTATACAATTGGTGCATGGTTTTATGCACAAAAG
    AATAGACCATATTTTCATATGATTTGGCATATTTTTATTGTAATAGCATCACTATTGCAT
    TTAATTGCAATCTTATATTTTATGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    687

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2071 [new locus tag: NWMN_RS12000 ]
  • symbol: NWMN_2071
  • description: hemolysin III
  • length: 228
  • theoretical pI: 9.66153
  • theoretical MW: 25806.8
  • GRAVY: 0.933772

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    channel protein, hemolysin III family (TIGR01065; HMM-score: 176)
  • TheSEED:  
    Hemolysin III 
  • PFAM:
    GPCR_A (CL0192) HlyIII; Haemolysin-III related (PF03006; HMM-score: 104.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.144
    • Ymax_pos: 56
    • Cmax: 0.164
    • Cmax_pos: 56
    • Smax: 0.26
    • Smax_pos: 1
    • Smean: 0.12
    • D: 0.134
  • predicted transmembrane helices (TMHMM): 7

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MMSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLVLPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMIYVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMGWVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLLHLIAILYFM

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: CcpA, LexA
    CcpA (TF) important in Carbon catabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise
    LexA (TF) important in SOS response  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]