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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2072 [new locus tag: NWMN_RS12005 ]
  • pan locus tag?: SAUPAN005560000
  • symbol: NWMN_2072
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2072 [new locus tag: NWMN_RS12005 ]
  • symbol: NWMN_2072
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2308316..2309503
  • length: 1188
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCTAGATAAAAATCAATTAGCTAAATATAAACAAGATCATTTGTGTGAATATGAAAAA
    ATAATGAGTAACAATGAAAAAGAAGCACTTGAAGAGAAGGTAGCGTCATTAGATTTAGAT
    TTTATTGCCAAATTATATAACGATTTATACATCAATAAGAAAACAATAGATGATGTATCT
    GCAGTTTCTGAAGTGAAATATGATATTAAATCACAAATGAGTGATGATGAGATTAAGCGT
    TTAGAGGAACAAGGACTTCAAGCTATTAAAGAAGGACAATTTGCAGTACTTTTGATGGCA
    GGTGGTCAAGGAACAAGACTTGGTTACAAGGGCCCTAAAGGTTCGTTTGAAATTGAAGGT
    GTAAGTTTATTTGAACTTCAAGCTAACCAATTAAAAACATTGAATCATCAATCTGGACAT
    ACGATTCAATGGTATATTATGACAAGTGATATCAATCATGAAGAAACTTTAGCTTATTTC
    GAAGCACATAGTTATTTTGGATATGATCAAGAAGCAATCCATTTCTTTAAACAAGATAAT
    ATTGTGGCGCTTAGTGAAGAAGGGAAGTTAATTTTAAATCAACAGGGTCGTATTATGGAA
    ACGCCGAATGGTAATGGTGGCGTGTTTAAATCTTTAGACAAGGCAGGATACTTAGAAGAA
    ATGTCTAATAATGGCGTTAAGTATATTTTCTTGAATAATATCGACAATGTTTTAGTAAGA
    GTGTTGGATCCACTATTTGCAGGATTTACGGTAGAACATGATTACGATATTACTTCTAAA
    ACGATTCAACCAAAACCAGGAGAAAGTGTTGGACGTTTAGTTAATGTCGATTGTAAAGAT
    ACAGTACTGGAGTATTCAGAATTAGATCCAGAAGTTGCAAATCAATTTAACAATGCTAAT
    ATCGGTATTCATGCATTTAAACTTGGTTTTATTTTAAACGCTGTAAATCGTGAGTTACCT
    TATCATTTAGCTATTAAGAATTTGAAGCAATTAGATGAGAACTTTGGTGTTATTGAACAA
    CCAACTTTAAAATTCGAATTGTTCTATTTTGACATATTTACTTATGGAACTAGTTTTGTC
    ACGTTACAAGTTCCTAGAGAAGAAGAATTTTCACCTCTTAAAAATAAAGAAGGTAAAGAT
    AGTGTTGCAACTGCAACAGAAGATTTACGTAGAATGGGTTTAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1188

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2072 [new locus tag: NWMN_RS12005 ]
  • symbol: NWMN_2072
  • description: hypothetical protein
  • length: 395
  • theoretical pI: 4.60106
  • theoretical MW: 44893.4
  • GRAVY: -0.391899

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Hypothetical proteinsConservedTIGR00454 family protein (TIGR00454; HMM-score: 17)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMolybdopterinmolybdenum cofactor guanylyltransferase (TIGR02665; EC 2.7.7.77; HMM-score: 13)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (TIGR00453; EC 2.7.7.60; HMM-score: 11.2)
  • TheSEED:  
    Cell Wall and CapsuleCell Wall and Capsule - no subcategoryPeptidoglycan Biosynthesis N-acetylglucosamine-1-phosphate uridyltransferase eukaryotic (EC 2.7.7.23) 
    Cell Wall and Capsule - no subcategoryUDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis N-acetylglucosamine-1-phosphate uridyltransferase eukaryotic (EC 2.7.7.23) 
  • PFAM:
    GT-A (CL0110) UDPGP; UTP--glucose-1-phosphate uridylyltransferase (PF01704; HMM-score: 141.1)
    NTP_transf_3; MobA-like NTP transferase domain (PF12804; HMM-score: 17.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_1587(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    NWMN_2063hypothetical protein  [1] (data from MRSA252)
    NWMN_2064hypothetical protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.116
    • Ymax_pos: 18
    • Cmax: 0.104
    • Cmax_pos: 31
    • Smax: 0.19
    • Smax_pos: 7
    • Smean: 0.124
    • D: 0.119
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAKLYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGGQGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYFEAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYLEEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNVDCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDENFGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRMGLI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: CcpA, LexA
    CcpA (TF) important in Carbon catabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise
    LexA (TF) important in SOS response  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2

Relevant publications[edit source | edit]