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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS04655 [old locus tag: SAUSA300_0862 ]
  • pan locus tag?: SAUPAN003065000
  • symbol: SAUSA300_RS04655
  • pan gene symbol?: glpQ
  • synonym:
  • product: glycerophosphodiester phosphodiesterase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS04655 [old locus tag: SAUSA300_0862 ]
  • symbol: SAUSA300_RS04655
  • product: glycerophosphodiester phosphodiesterase
  • replicon: chromosome
  • strand: -
  • coordinates: 940901..941830
  • length: 930
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACTAACTCTTCGAAAAGCTTCACTAAATTTATGGCTGCTTCTGCTGTTTTTACTATG
    GGATTTTTATCAGTACCTACTGCTGGCGCTGAACAAACAAATCAAATTGCAAATAAACCT
    CAGGCTATTCAATGGCATACAAATTTAACGAATGAGCGATTCACTACTATCGCACATCGT
    GGCGCAAGTGGCTATGCACCCGAGCATACGTTTCAAGCATATGATAAGAGTCATAATGAG
    TTAAAAGCATCTTATATCGAAATTGATTTACAACGTACCAAAGATGGCCATTTAGTTGCT
    ATGCATGATGAAACTGTTAACCGTACAACAAATGGACACGGTAAAGTTGAGGATTATACC
    CTTGATGAATTAAAACAGTTAGATGCAGGAAGTTGGTTTAATAAAAAATATCCAAAATAC
    GCAAGAGCAAGTTATAAAAATGCTAAAGTACCCACTTTAGATGAAATTTTAGAACGTTAT
    GGCCCGAATGCAAACTATTATATTGAAACAAAGTCACCTGATGTATACCCAGGAATGGAA
    GAACAATTATTAGCTTCATTGAAAAAGCATCACCTTTTAAATAACAATAAATTAAAAAAT
    GGACATGTAATGATTCAATCATTTTCTGACGAAAGTTTAAAGAAAATTCATCGTCAAAAT
    AAGCATGTGCCATTAGTAAAATTAGTTGATAAAGGTGAACTACAACAATTTAACGACCAA
    CGCTTAAAAGAGATACGCTCTTATGCGATTGGATTAGGTCCTGATTATACAGATTTAACT
    GAACAAAATACCCATCATTTAAAAGACTTAGGATTTATAGTACATCCTTATACAGTGAAT
    GAAAAAGCTGATATGTTACGATTAAATAAATATGGCGTTGATGGTGTCTTTACAAATTTC
    GCTGATAAATATAAAGAAGTCATTAAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    930

Protein[edit | edit source]

Protein Data Bank: 2P76
Protein Data Bank: 2OOG

General[edit | edit source]

  • locus tag: SAUSA300_RS04655 [old locus tag: SAUSA300_0862 ]
  • symbol: SAUSA300_RS04655
  • description: glycerophosphodiester phosphodiesterase
  • length: 309
  • theoretical pI: 9.14583
  • theoretical MW: 35310.5
  • GRAVY: -0.731068

Function[edit | edit source]

  • reaction:
    EC 3.1.4.46?  ExPASy
    Glycerophosphodiester phosphodiesterase A glycerophosphodiester + H2O = an alcohol + sn-glycerol 3-phosphate
  • TIGRFAM:
  • TheSEED: see SAUSA300_0862
  • PFAM:
    PLC (CL0384) GDPD; Glycerophosphoryl diester phosphodiesterase family (PF03009; HMM-score: 162.5)
    and 1 more
    GDPD_2; Glycerophosphoryl diester phosphodiesterase family (PF13653; HMM-score: 16.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.3
    • Cellwall Score: 4.55
    • Extracellular Score: 5.15
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.828547
    • TAT(Tat/SPI): 0.163998
    • LIPO(Sec/SPII): 0.002972
    • Cleavage Site: CS pos: 30-31. AGA-EQ. Pr: 0.8839
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQWHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMHDETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERYGPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHRQNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHPYTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]