NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS08785 [old locus tag: SAUSA300_1612 ]
• pan locus tag^?: SAUPAN004277000
• symbol: SAUSA300_RS08785
• pan gene symbol^?: tag
• synonym:
• product: DNA-3-methyladenine glycosylase I

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS08785 [old locus tag: SAUSA300_1612 ]
• symbol: SAUSA300_RS08785
• product: DNA-3-methyladenine glycosylase I
• replicon: chromosome
• strand: +
• coordinates: 1765506..1766066
• length: 561
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (1765506..1766066) NCBI
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  
  ATGAATGAATGCGCATTTGGTACTAAAGATCCAGTCTACTTAGACTATCATGATCATGTA
  TGGGGACAACCGCTCTATGATAGCAAGGCATTGTTTAAACTTTTAGCATTAGAATCACAA
  CATGCTGGGCTATCTTGGTTAACTATTTTAAAAAAGAAAGAAGCCTATGAAGAAGCATTT
  TATGATTTCGAACCAGAAAAGGTAGCACAAATGACCGCTCAAGATATCGACCGCTTAATG
  ACTTTTCCAAATATCGTTCATCATCGTAAAAAATTAGAAGCAATTGTTAATCAAGCTCAA
  GGGTATTTAAAAATTGAACAAGCATATGGTAGTTTTAGTAAATTTTTATGGTCATATGTA
  AATGGTAAGCCTAAAGATTTGCAGTATGAACATGCTTCTGATCGTATCACAGTTGATGAT
  ACTGCAACACAACTATCTAAAGATTTAAAACAATACGGGTTTAAATTTTTAGGTCCAGTA
  ACAGTATTTTCGTTTTTAGAAGCAGCCGGTTTATATGATGCACATTTACAAGATTGTCCA
  TCAAAGCCTAAACATAATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  561
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS08785 [old locus tag: SAUSA300_1612 ]
• symbol: SAUSA300_RS08785
• description: DNA-3-methyladenine glycosylase I
• length: 186
• theoretical pI: 6.50996
• theoretical MW: 21416.1
• GRAVY: -0.476882

⊟Function

• reaction:
  EC 3.2.2.-?  ExPASy
• TIGRFAM:
  Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA-3-methyladenine glycosylase I (TIGR00624; EC 3.2.2.20; HMM-score: 190.7)
• TheSEED:
• PFAM:
  no clan definedAdenine_glyco; Methyladenine glycosylase (PF03352; HMM-score: 215)
  and 2 more
  COOH-NH2_lig; Phage phiEco32-like COOH.NH2 ligase-type 2 (PF14395; HMM-score: 11.7)
  DUF3326; Protein of unknown function (DUF3326) (PF11805; HMM-score: 11)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • Ymax: 0.119
  • Ymax_pos: 56
  • Cmax: 0.121
  • Cmax_pos: 56
  • Smax: 0.189
  • Smax_pos: 41
  • Smean: 0.077
  • D: 0.103
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446923972 NCBI
• RefSeq: WP_001001228 NCBI
• UniProt:

⊟Protein sequence

• MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGLSWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGYLKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPVTVFSFLEAAGLYDAHLQDCPSKPKHN

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• SAUSA300_RS08785 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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