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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS11610 [old locus tag: SAUSA300_2106 ]
  • pan locus tag?: SAUPAN005496000
  • symbol: SAUSA300_RS11610
  • pan gene symbol?: mtlR
  • synonym:
  • product: PTS lactose transporter subunit IIB

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_RS11610 [old locus tag: SAUSA300_2106 ]
  • symbol: SAUSA300_RS11610
  • product: PTS lactose transporter subunit IIB
  • replicon: chromosome
  • strand: +
  • coordinates: 2274254..2276386
  • length: 2133
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_007793 (2274254..2276386) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    ATGTTATTGAGTACACGTGAAAAAGAAATGATAGCCCTATTGATTAAGTACCACGGTCAA
    TATATCACTATACACGACATTGCTCAGCAACTTGCGGTGTCCTCTCGTACTATTCACCGT
    GAATTAAAAGGTGTTGAAGCATATTTAACTTCATTTTCATTAACTTTAGAACGCGCAAAC
    AAAAAAGGGCTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATT
    GCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCT
    TTGATACAAGCCAAGGAGCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCT
    GTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTA
    TCTTTATCTCGAAAGCGTGGCGAAGGCATTTACTTGGTAGGTACTGAATCAAAGAAACGT
    GAATTTTTAAGTCAATTAATGGTGAATAACTTAAATAGTACTAGCGTTTATTCAGTAATT
    GAAAATCATTTTGTCTTTCATTCATTAAATCAAATCCACAAAGACTTTGTTGACTTAGAG
    CGCATTTTTAATGTTGAAAGACTATTAATGGACTACCTAAGTGCCTTACCCTACCAACTT
    ACCGAATCAAGTTATTTAACTTTAACTGTCCATATCGTGCTCTCCATTTCACGTATAAAA
    AATGGAGAGTATGTCGCATTAAACGATGATATTTATGATTCTGTACAAAACACATTTGAA
    CACAAAGTAGCAAGCGAACTTGCTGATAAACTTGGTCAAATATATGACGTCACGTTTAAT
    CAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAAT
    GATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTA
    GAAATGATTTCCGGTATGACATTTGCAGATTTGGATACTTTAGTAGATGGACTGACGCTA
    CACCTTAATCCTGCAATCAATCGTTTGCAAGCTAATATCGAGACCTATAATCCGTTAACA
    GACATGATTAAGTTCAAATATCCAAGACTATTTGAAAATGTAAGATTAGCTTTAAATGAT
    TGTTGGCCTGATTTGATTTTTCCAGAGAGTGAAATTGCTTTTATAGTTTTACACTTTGGT
    GGCTCGATTAAAAACCAAGGTAATCGATTTTTAAACATATTAGTCGTTTGCAGCAGTGGT
    ATGGGAACTAGTCGTCTATTATCAACTCGTCTAGATCAAGTTTTTAGTGAGATTGAGCGT
    ATTACACAAGCATCAGTCAGCGATTTGAAGTCACTAGATTTAAGTCAATATGATGGCATT
    ATTTCTACTGTGAATTTAGACATCGACTCCCCCTATTTAACGGTAAACCCATTATTACCA
    GATAGTGATATCAGTTATGTCGCACAGTTTTTAAATACAAAGTCTACGTTCCAAGAGACG
    CATGATAAATCATCAAACATGATTGATAAGGATGATGTTCATGTTGAAACGAAAGATGTT
    GATGGCAACACATCTTTTGAAAATGAACAAACTTCATACTTAACTTCAGTTTTCGAAAAA
    CATTTAAGTGACGAAAAATCAGAACAATTATTGCATCATATGCGTTCGGGTTTAACTTTG
    CTTGATTCAGTGAAAATAGTTAGTACCGAAGTTAAACAGTGGCAAACATATATCGCAGAT
    TATCTATATCAATGCGATGTAATAAACGATCCAACGTCATTCGCTGAACTACTAGAGCAA
    CGATTGATTGACAATCCAGGATGGATATTAAGTCCATATCCTGTTGCAATACCACACCTA
    AGAGACAATATGATTAAACACCCTATGATTCTAATCACAGTTTTAGAAGAACCGTTAACA
    TTGCCTAGTATTCAAAATGACAATCAAACAATTAAATATATGATTTCCATGTTTATTTCT
    GACAATGATTTTATGGCATCACTGGTAAGTGACTTGTCCGAATTTTTAAGTTTGAAATTA
    GAATCTATTGATACTTTTATGGAAAATCCACAGGAACTTGAAACATTATTAAGAAACAAA
    TTTTTAGAACGAATTAAAAAACAATTTATTTAG
    60
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    2133

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_RS11610 [old locus tag: SAUSA300_2106 ]
  • symbol: SAUSA300_RS11610
  • description: PTS lactose transporter subunit IIB
  • length: 710
  • theoretical pI: 4.88328
  • theoretical MW: 81131
  • GRAVY: -0.175493

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 28)
    Signal transductionSignal transductionPTSPTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 28)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtheriron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 16.3)
    Signal transductionRegulatory functionsDNA interactionsiron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 16.3)
    RNA polymerase sigma-70 factor, sigma-B/F/G subfamily (TIGR02980; HMM-score: 16)
    RNA polymerase sigma factor, TIGR02999 family (TIGR02999; HMM-score: 13.6)
    RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 13)
    Unknown functionEnzymes of unknown specificityNDMA-dependent alcohol dehydrogenase, Rxyl_3153 family (TIGR03989; EC 1.1.99.36; HMM-score: 12.9)
    probable regulatory domain (TIGR03879; HMM-score: 12.6)
  • TheSEED:
  • PFAM:
    PRD (CL0166) PRD; PRD domain (PF00874; HMM-score: 118.2)
    HTH (CL0123) Mga; Mga helix-turn-helix domain (PF05043; HMM-score: 56.6)
    HTH_11; HTH domain (PF08279; HMM-score: 50.1)
    PTase-anion_tr (CL0340) PTS_EIIA_2; Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2 (PF00359; HMM-score: 34.4)
    Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 26.8)
    HTH (CL0123) HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 21.1)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 20.5)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 18.3)
    IF2_N; Translation initiation factor IF-2, N-terminal region (PF04760; HMM-score: 18)
    HTH_10; HTH DNA binding domain (PF04967; HMM-score: 16.9)
    DUF2089; Protein of unknown function (DUF2089) (PF09862; HMM-score: 16.9)
    HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 16.4)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 15.9)
    MarR; MarR family (PF01047; HMM-score: 15.6)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 14.9)
    HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 14.4)
    HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 13.5)
    GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 13.4)
    no clan definedDUF746; Domain of Unknown Function (DUF746) (PF05344; HMM-score: 13.1)
    DUF2675; Protein of unknown function (DUF2675) (PF11247; HMM-score: 13)
    HTH (CL0123) SMC_ScpB; Segregation and condensation complex subunit ScpB (PF04079; HMM-score: 12.5)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.121
    • Ymax_pos: 11
    • Cmax: 0.107
    • Cmax_pos: 29
    • Smax: 0.233
    • Smax_pos: 2
    • Smean: 0.142
    • D: 0.129
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446850104 NCBI
  • RefSeq: WP_000927360 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKGVEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALIQAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKREFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPYQLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYDVTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTLVDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEIAFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLDQVFSEIERITQASVSDLKSLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMIDKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKIVSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNMIKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFISDNDFMASLVSDLSEFLSLKLESIDTFMENPQELETLLRNKFLERIKKQFI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAUSA300_RS11610 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]