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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS12235 [old locus tag: SAUSA300_2217 ]
  • pan locus tag?: SAUPAN005723000
  • symbol: SAUSA300_RS12235
  • pan gene symbol?:
  • synonym:
  • product: MFS transporter

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS12235 [old locus tag: SAUSA300_2217 ]
  • symbol: SAUSA300_RS12235
  • product: MFS transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 2386904..2388115
  • length: 1212
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACTAATCAAGGCTCATTAAATAAATCACCTATTTTCACCAAAAGTTTTACAATTAAC
    TTTTTAGTCAACTTTATCGTATACCTATGCATGTATTTGTTACTTGTAGTTATTGCTGGC
    TATAGTAAGCAAGCTTTCGACGCTTCCGATTCATTAGCCGGATTAGTTGTAGGACTATTT
    ATTGTCGGGTCTTTAATTGGTCGATTTGCTACAGGTAAATTTGTAAATCAAATCGGACCT
    AAACGATTATTATTTATTGGTTTAATTGCTTTAATTATCACGCAATTACTTTATTTTATT
    GATGGATCTTTAGCTTTTTTAATCTTTGTACGTCTCATAAACGGTATCGCAACTGCAGTT
    GTGACAACTGCAACAGGTACTATTGCGGCATACGTTACCCCTGTTAATAGAAAAAGTGAA
    GGCATCAGTTTATTTTCCTTAAGTTTAGTATTAGGTACAGCGATTGGCCCATTTCTAGGC
    ATGTTACTTATTACAAAATACGCAATTGACTTACTGTTTATTATTTGTGTCATATTAGGC
    ATACTCGGACTCATTATCTCATTATTTATAAAAGTTGATTTCGAAGTAACAAATAACAAA
    ACTGAAACTAATGTCATTGATAAACCAAGATTTAGTATTCATCAATTTATTGCAAAAGAG
    GCCATTCCTGTTGCTGTAATTATGCTTTTAATTGGCGTTACCTATGCGTCCATCCTAACT
    TATTTACAAGCATTTGCTTTAGAGCGACACCTAGTCACTGCAGCAAGCTATTTCTTTATT
    TGCTATGCTATAGCATCGTTAATTACTAGACCTATTGCTGGTAGATTGATGGATGATAAA
    AATGAAAATATTATTGTCTACCCAGCATTTATCATGCTTTTCCTTTCATTTGTATGCTTA
    ATTTCAAGCTATCAAAGCTGGTTAATATTACTTGCTGGTGCTTGCCTAGGTTTAGGGTAT
    GGAAATTTATCATCTGCAATGCAATCTATTGCGATAAAAGTCTCACCCCCGATTAAATAT
    GGTATCGCGACATCTACTTTTTACGTTGGTCTAGATGCAGGTGTCGGCTTCGGTCCATCA
    TTCCTTGGGTTATTTACTCATATGTTTTCATATAGTGAAATCTTCGGCTTTATGGCCGCT
    TTAGCAATCATTACTATGCTTGTTTACTTCTTAATTCATGGACGTCATGTAACTAGAAAT
    GCCATGAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1212

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS12235 [old locus tag: SAUSA300_2217 ]
  • symbol: SAUSA300_RS12235
  • description: MFS transporter
  • length: 403
  • theoretical pI: 9.3132
  • theoretical MW: 43887.2
  • GRAVY: 1.00422

Function[edit | edit source]

  • TIGRFAM:
    multidrug resistance protein (TIGR00880; HMM-score: 72.5)
    and 11 more
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 54.6)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 54.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 45.3)
    Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 34.5)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sugar porter (SP) family (TIGR00879; HMM-score: 29.9)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 29.3)
    Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 29.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 28.2)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 27.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids sugar efflux transporter (TIGR00899; HMM-score: 21.7)
    Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 20.9)
  • TheSEED: see SAUSA300_2217
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 155.2)
    and 4 more
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 38.2)
    MFS_1_like; MFS_1 like family (PF12832; HMM-score: 32.7)
    no clan defined DUF5357; Family of unknown function (DUF5357) (PF17310; HMM-score: 7.8)
    MFS (CL0015) MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008854
    • TAT(Tat/SPI): 0.000287
    • LIPO(Sec/SPII): 0.005548
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTNQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQAFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDGSLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLGMLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIAKEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRLMDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKVSPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLIHGRHVTRNAMN

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]