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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS00665 [old locus tag: SA0102 ]
  • pan locus tag?: SAUPAN000902000
  • symbol: SA_RS00665
  • pan gene symbol?:
  • synonym:
  • product: oleate hydratase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA_RS00665 [old locus tag: SA0102 ]
  • symbol: SA_RS00665
  • product: oleate hydratase
  • replicon: chromosome
  • strand: -
  • coordinates: 115472..117247
  • length: 1776
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002745 (115472..117247) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTATTACAGTTATGGAAATTATGAAGCATTTGCGCGCCCTAAAAAACCTGAAAATGTA
    GAAAACAAATCTGCTTACTTAATCGGGTCTGGGTTAGCTTCACTTGCGGCAGCTTGTTTC
    TTAATTAGGGATGGTCAAATGGAAGGTTCGAAGATTCATATTTTAGAAGAGTTACCTAAA
    GCAGGTGGTAGTCTTGATGGTGAAAATATGCCTTTAAAAGGCTATGTTGTCCGCGGTGGT
    CGTGAAATGGAGAACCACTTTGAATGTTTGTGGGACTTATTCAGATCTATCCCTTCATTA
    GAAATCGATAACGCGTCTGTATTAGATGAGTTCTATTGGCTAAACAAAGAAGACCCTAAC
    TATTCTCGCTGTCGTGTTATTGAGAAACAGGGTCAACGTTTAGTCACAGACGGAGACTTC
    ACTTTGACTAAAACGGCGATTAAAGAAATTTTAGATTTATGCTTAACGAATGAAGAAGAT
    TTAGATGATGTCAAAATAACAGATGTATTTTCCGATGACTTCTTTAATTCAAACTTTTGG
    ATTTACTGGAAAACGATGTTTGCATTTGAACCGTGGCATTCTGCTATGGAAATGCGTCGT
    TATCTAATGCGATTCGTTCATCATATTGGTGGTCTCGCAGACTTTTCAGCTTTAAAATTC
    ACTAAATATAATCAATATGAATCTTTAGTATTACCTATGGTTGAATATTTAAAATCGCAT
    GGGGTTCAATTTGAATACGATGTAAAAGTAGAAGATATTAAAGTAGATGTTACGACAAGT
    CAAAAAATTGCCCGAGAAATATTAATTCACCGACATGGCAAAGCAGAGTCAATTAAACTG
    ACTGTAGATGATCTTGTCTTTGTGACAAACGGTAGTATTACGGAAAGCTCTACGTATGGT
    GATAATGATACACCAGCACCACCAACTGACGAATTAGGTGGTAGTTGGACACTATGGAAA
    AATTTAGCGCGACAAAGTCCTGAATTTGGTAATCCTGATAAGTTTTGCCAAAATATTCCT
    AAAAAAAGTTGGTTTGTTTCAGCAACTTCTACAACAAACAATAAAGAGATTATCGATACA
    ATAGAAAGTATTTGTAAACGTGACCCACTTGCAGGCAAAACAGTTACAGGCGGTATTATT
    ACAATCAATGATTCTGCATGGCAAATGAGTTTTACAATCAATCGTCAGCAACAGTTTAAA
    GACCAACCTGAAAATGAAATATCTACATGGATTTATGCCTTATATTCAGATGTAAACGGC
    GATTATATTAAAAAGCCAATTACAGAATGTAGTGGTAATGAAATATGCCAAGAATGGCTG
    TATCACTTAGGTGTATCAACTGACAAAATTGAAGACTTAGCAAAACATGTATCTAATACG
    ATTCCTGTTTATATGCCATATATCACCTCTTATTTCATGACGCGTGCTATCGGCGACAGA
    CCTTTAGTCGTCCCGCATCAATCACAAAATTTATCATTTATTGGTAACTTTGCAGAAACA
    GAGCGAGATACTGTATTTACAACAGAATATTCGGTTCGTACTGCCATGGAAGCTGTTTAT
    CAATTACTAAATATAGATCGTGGTATTCCAGAGGTCATCAATAGTACATTTGATCTTCGC
    GTCTTAATGGATGCCGTATACGAACTGAATGACCATCAAGATTTGCGCGAGATTACTAAA
    GATTCGAAAATGCAGAAACTCGCATTAGCAGGATTCCTTAAAAAGATAAAAGGTACGTAC
    ATTGAGACATTATTAAAAGAACACAAATTGTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1776

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA_RS00665 [old locus tag: SA0102 ]
  • symbol: SA_RS00665
  • description: oleate hydratase
  • length: 591
  • theoretical pI: 4.88159
  • theoretical MW: 67698.3
  • GRAVY: -0.370389

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    9,9'-di-cis-zeta-carotene desaturase (TIGR02732; EC 1.3.5.6; HMM-score: 15.3)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 14.7)
  • TheSEED:
  • PFAM:
    NADP_Rossmann (CL0063) MCRA; MCRA family (PF06100; HMM-score: 743.8)
    and 3 more
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 25)
    no clan definedGlyco_hydro_59M; Glycosyl hydrolase family 59 central domain (PF17387; HMM-score: 14.1)
    P-loop_NTPase (CL0023) AAA_15; AAA ATPase domain (PF13175; HMM-score: 13.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.141
    • Ymax_pos: 39
    • Cmax: 0.122
    • Cmax_pos: 39
    • Smax: 0.232
    • Smax_pos: 42
    • Smean: 0.127
    • D: 0.136
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446213985 NCBI
  • RefSeq: WP_000291840 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRDGQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEIDNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEEDLDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGLADFSALKFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKVDVTTSQKIAREILIHRHGKAESIKLTVDDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDKFCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFTINRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKIEDLAKHVSNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLSFIGNFAETERDTVFTTEYSVRTAMEAVYQLLNIDRGIPEVINSTFDLRVLMDAVYELNDHQDLREITKDSKMQKLALAGFLKKIKGTYIETLLKEHKLL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA_RS00665 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]