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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS01095 [old locus tag: SA0183 ]
  • pan locus tag?: SAUPAN001032000
  • symbol: SA_RS01095
  • pan gene symbol?: glcA
  • synonym:
  • product: PTS glucose EIICBA component

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA_RS01095 [old locus tag: SA0183 ]
  • symbol: SA_RS01095
  • product: PTS glucose EIICBA component
  • replicon: chromosome
  • strand: -
  • coordinates: 214814..216859
  • length: 2046
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002745 (214814..216859) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    GTGAGGAAGAAACTTTTCGGTCAATTGCAACGTATTGGTAAAGCGCTAATGTTACCTGTT
    GCAATTTTACCAGCAGCGGGTCTGTTATTAGCTATCGGTACAGCTATACAAGGCGAAGCA
    TTACAACACTACTTGCCGTTTATACAAAATGGTGGCGTTCAAAATGTCGCTAAATTAATG
    ACAGCTGCCGGTAGTATTATTTTTGAAAACTTACCTATGATTTTCGCATTAGGTGTCGCA
    ATCGGATTAGCTGGCGGTGATGGCGTAGCAGCTATCGCAGCATTCGTCGGTTACATAATC
    ATGAACAAAACAATGGGCGACTTTTTACAAGTTACACCTAAAAATGTTACTGATCCAGCG
    AGTGGTTACGCTAGCATTTTAGGTATTCCAACATTACAAACAGGTGTGTTCGGCGGTATT
    ATTATCGGGGCCCTGGCAGCTTGGTGTTATAACAAGTTCTATAACATTAACTTACCATCT
    TATTTAGGTTTCTTCGCTGGTAAGCGTTTCGTACCTATTATGATGGCTACAACATCATTT
    ATTTTAGCATTTCCAATGGCATTAATTTGGCCAACGATTCAATCAGGATTAAATGCATTC
    AGTACAGGATTATTAGATTCAAATACTGGTGTTGCCGTATTCTTATTTGGTTTCATCAAG
    CGTTTATTAATTCCATTCGGTCTACATCACATTTTCCACGCACCGTTCTGGTTCGAGTTT
    GGTTCATGGAAAAATGCAGCTGGTGAAATTATTCACGGTGACCAACGTATCTTTATCGAA
    CAAATTCGTGAAGGCGCACATTTGACAGCTGGTAAATTCATGCAAGGTGAATTCCCTGTT
    ATGATGTTCGGTTTACCTGCAGCAGCTTTAGCAATTTATCACACAGCTAAACCTGAAAAT
    AAGAAAGTAGTAGCAGGTTTAATGGGTTCTGCTGCTTTAACATCATTCTTAACTGGTATT
    ACAGAACCTTTAGAATTCTCATTCTTATTCGTAGCACCATTATTATTCTTTATTCACGCA
    GTACTTGATGGTTTATCATTCTTAACATTGTACTTATTGGATGTTCACTTAGGTTATACT
    TTCTCAGGTGGTTTCATCGACTACGTTTTACTTGGCGTACTACCTAATAAGACACAATGG
    TGGTTAGTCATTCCTGTAGGTCTTGTATACGCAGTTATTTACTACTTCGTATTCCGATTC
    TTAATTGTAAAATTAAAATACAAAACACCAGGTCGTGAAGATAAGCAATCACAAGCTGTT
    ACTGCTTCAGCAACTGAATTACCATATGCAGTATTAGAAGCTATGGGTGGCAAAGCAAAC
    ATTAAACATTTAGACGCTTGTATCACACGTCTACGTGTTGAAGTTAACGACAAATCTAAA
    GTTGATGTTCCTGGTTTGAAAGATTTAGGCGCATCTGGTGTATTAGAAGTCGGCAATAAT
    ATGCAAGCAATTTTTGGTCCTAAATCTGACCAAATCAAACATGAAATGCAACAGATTATG
    AATGGTCAAGTAGTAGAAAACCCTACTACTATGGAAGACGATAAAGACGAAACTGTTGTT
    GTTGCAGAAGATAAATCTGCAACAAGCGAATTGAGCCATATCGTTCATGCACCATTAACT
    GGTGAAGTAACACCTTTATCAGAAGTGCCTGATCAAGTGTTCAGCGAAAAAATGATGGGT
    GACGGTATCGCTATTAAACCTTCACAAGGTGAAGTTCGTGCACCATTCAACGGTAAAATA
    CAAATGATTTTCCCAACAAAACATGCAATTGGTCTTGTATCAGATAGTGGTTTAGAACTA
    TTAATCCACATCGGTTTAGACACTGTTAAATTAAACGGAGAAGGCTTTACTTTACATGTT
    GAGGAAGGTCAAGAAGTTAAACAAGGTGATTTATTAATCAACTTTGATTTAGACTACATC
    CGCAATCATGCAAAGAGTGATATTACGCCTATTATCGTGACACAAGGAAACATTACAAAC
    CTTGATTTTAAACAAGGTGAACATGGCAACATTTCATTTGGCGATCAATTATTTGAAGCT
    AAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2046

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA_RS01095 [old locus tag: SA0183 ]
  • symbol: SA_RS01095
  • description: PTS glucose EIICBA component
  • length: 681
  • theoretical pI: 6.33084
  • theoretical MW: 73957.7
  • GRAVY: 0.330837

Function[edit source | edit]

  • reaction:
    EC 2.7.1.199?  ExPASy
    Protein-N(pi)-phosphohistidine--D-glucose phosphotransferase[Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) = [protein]-L-histidine + D-glucose 6-phosphate(Side 2)
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 799.9)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 478.9)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 401.2)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 286.3)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 278.7)
    Signal transductionSignal transductionPTSPTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 278.7)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 243.9)
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 210)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 165.8)
    Signal transductionSignal transductionPTSPTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 165.8)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.4)
    Signal transductionSignal transductionPTSPTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.4)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 62.6)
    PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 28.4)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 16.1)
    MetabolismTransport and binding proteinsUnknown substrateefflux transporter, RND family, MFP subunit (TIGR01730; HMM-score: 14.6)
  • TheSEED:
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 329.9)
    Hybrid (CL0105) PTS_EIIA_1; phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1 (PF00358; HMM-score: 181.7)
    no clan definedPTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 53.2)
    Hybrid (CL0105) Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 20)
    no clan definedPIG-P; PIG-P (PF08510; HMM-score: 16.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.244
    • Ymax_pos: 15
    • Cmax: 0.128
    • Cmax_pos: 39
    • Smax: 0.718
    • Smax_pos: 13
    • Smean: 0.56
    • D: 0.367
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit source | edit]

  • GI: 447150453 NCBI
  • RefSeq: WP_001227709 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAIQGEALQHYLPFIQNGGVQNVAKLMTAAGSIIFENLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMNKTMGDFLQVTPKNVTDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPSYLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGFIKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQGEFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPLLFFIHAVLDGLSFLTLYLLDVHLGYTFSGGFIDYVLLGVLPNKTQWWLVIPVGLVYAVIYYFVFRFLIVKLKYKTPGREDKQSQAVTASATELPYAVLEAMGGKANIKHLDACITRLRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENPTTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIKPSQGEVRAPFNGKIQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQEVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA_RS01095 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]