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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS02465 [old locus tag: SA0433 ]
  • pan locus tag?: SAUPAN002191000
  • symbol: SA_RS02465
  • pan gene symbol?: treC
  • synonym:
  • product: glucohydrolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA_RS02465 [old locus tag: SA0433 ]
  • symbol: SA_RS02465
  • product: glucohydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 498965..500605
  • length: 1641
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002745 (498965..500605) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
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    841
    901
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    GTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGTATATCAAATTTATCCTAAGTCGTTT
    AATGATACGACGGGGAATGGTATAGGAGATATCAACGGAATTATAGAAAAATTGGATTAT
    ATCAAGTTATTGGGTGTTGATTATATTTGGTTAACACCAGTGTATGAATCACCTATGAAT
    GATAATGGCTATGATATCAGCAATTATTTAGAAATCAATGAAGCCTTTGGAACGATGGAT
    GATTTTGAAAAGTTAATCAAAGTTGCTCATCAAAAAGACTTGAAAGTGATGTTAGATATT
    GTTATTAATCATACGTCGACGGAGCATGAATGGTTTAAAGAAGCCCGTAAATCTAAAGAT
    AACCCTTATAGAGATTATTACTTTTTCAGATCATCTGAAGACGGGCCGCCAACAAATTGG
    CATTCTAAATTCGGTGGTAATGCATGGAAGTATGATTCTGAGACAGATGAATATTATTTA
    CATTTATTTGATGTCAGTCAAGCTGATTTAAATTGGGATAATCCGGAAGTACGTCAATCG
    TTATATCGCATAGTCAATCATTGGATAGACTTCGGCGTTGATGGTTTTCGATTTGATGTC
    ATTAACTTAATTTCTAAAGGTGAATTTAAGGACTCTGACAAAATAGGTAAAGAATTTTAT
    ACGGATGGTCCTAGAGTGCATGAGTTTCTGCATGAATTAAATCGTCAAACGTTTGGTAAC
    ACTGACATGATGACTGTAGGAGAAATGTCTTCGACGACGATTGAAAATTGTATTAAGTAT
    ACACAACCAGAACGCCAAGAATTGAATAGTGTTTTTAATTTTCATCATCTAAAGGTTGAT
    TATGTTGATGGTGAAAAGTGGACAAATGCGAAGCTTGATTTTCATAAGTTAAAGGAAATT
    CTGATGCAATGGCAACGAGGTATTTATGACGGTGGCGGATGGAACGCGATTTTCTGGTGT
    AATCATGATCAGCCACGGGTAGTGTCTAGATTTGGTGATGATACGTCGGAAGAGATGAGG
    ATACAAAGTGCTAAAATGTTAGCTATCGCACTGCATATGTTGCAAGGGACGCCATATATT
    TACCAAGGTGAAGAAATTGGTATGACGGACCCACATTTTACATCAATAGCACAATATCGT
    GATGTTGAATCGATTAATGCCTACCATCAGTTGCTAAGTGAAGGGCATGCTGAAGCGGAT
    GTATTAGCGATTTTAGGACAGAAGTCACGAGACAATTCGAGAACGCCTATGCAATGGAGT
    GATGATGTTAATGCTGGATTTACAGCTGGTAAGCCTTGGATTGATATTTCGGAAAATTAT
    CATCAGGTCAACGTTAGACAAGCACTTCAGAATAAAGAGTCTATTTTCTATACGTATCAA
    AAATTAATACAATTAAGACATACGCATGATATTATTACGTATGGAGACATTGTGCCACGT
    TTTATGGATCATGATCATTTATTTGTTTATGAACGTCATTATAAGAATCAACAATGGCTA
    GTAATTGCGAATTTCTCAGCATCGGCTGTTGATTTGCCAGAAGGATTGGCTAGAGAAGGT
    TGTGTTGTGATTCAAACAGGCACAGTGGAAAATAATACGATAAGCGGGTTTGGTGCAATT
    GTAATCGAAACAAACGCGTAA
    60
    120
    180
    240
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    720
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    1020
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    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1641

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA_RS02465 [old locus tag: SA0433 ]
  • symbol: SA_RS02465
  • description: glucohydrolase
  • length: 546
  • theoretical pI: 4.93396
  • theoretical MW: 63455.4
  • GRAVY: -0.566483

Function[edit source | edit]

  • TIGRFAM:
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 817.7)
    and 9 more
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidestrehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 294.6)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesmalto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 82.3)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesmalto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 57.1)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharides1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 37.6)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesglycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 35.2)
    sucrose phosphorylase (TIGR03852; EC 2.4.1.7; HMM-score: 32.7)
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 32.2)
    pullulanase, extracellular (TIGR02102; HMM-score: 20.8)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesglycogen debranching enzyme (TIGR01531; HMM-score: 19)
  • TheSEED:
  • PFAM:
    Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 412.4)
    and 4 more
    hDGE_amylase; Glycogen debranching enzyme, glucanotransferase domain (PF14701; HMM-score: 28.1)
    GHD (CL0369) Malt_amylase_C; Maltogenic Amylase, C-terminal domain (PF16657; HMM-score: 22.8)
    DUF3459; Domain of unknown function (DUF3459) (PF11941; HMM-score: 20.9)
    Cyc-maltodext_C; Cyclo-malto-dextrinase C-terminal domain (PF10438; HMM-score: 13.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.107
    • Ymax_pos: 28
    • Cmax: 0.106
    • Cmax_pos: 22
    • Smax: 0.154
    • Smax_pos: 25
    • Smean: 0.09
    • D: 0.1
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 445953635 NCBI
  • RefSeq: WP_000031490 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLLGVDYIWLTPVYESPMNDNGYDISNYLEINEAFGTMDDFEKLIKVAHQKDLKVMLDIVINHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKYDSETDEYYLHLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKEFYTDGPRVHEFLHELNRQTFGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHHLKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDDTSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLLSEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQNKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSASAVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA_RS02465 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]