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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03730 [old locus tag: SA0654 ]
  • pan locus tag?: SAUPAN002583000
  • symbol: SA_RS03730
  • pan gene symbol?: fruB
  • synonym:
  • product: 1-phosphofructokinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA_RS03730 [old locus tag: SA0654 ]
  • symbol: SA_RS03730
  • product: 1-phosphofructokinase
  • replicon: chromosome
  • strand: +
  • coordinates: 747089..748009
  • length: 921
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002745 (747089..748009) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGATTTATACAGTGACTTTCAATCCTTCAATTGACTATGTCATTTTTACGAATGATTTT
    AAAATTGATGGTTTGAACAGAGCAACAGCAACATATAAATTCGCTGGGGGGAAAGGTATT
    AATGTCTCGCGCGTCTTAAAGACATTAGATGTTGAGTCAACTGCCTTGGGATTTGCAGGT
    GGATTTCCTGGGAAATTCATTATAGATACATTAAATAACAGTGCAATTCAATCGAATTTT
    ATTGAAGTTGATGAAGATACACGTATTAATGTGAAATTAAAAACAGGACAAGAAACAGAA
    ATCAATGCACCGGGTCCTCATATAACGTCAACACAATTTGAACAACTGTTACAACAAATT
    AAAAATACAACAAGCGAAGATATAGTTATTGTTGCTGGAAGTGTACCAAGTAGTATTCCA
    AGCGATGCGTATGCGCAAATTGCACAAATTACAGCACAGACAGGTGCTAAATTAGTAGTC
    GACGCTGAAAAAGAATTGGCTGAAAGCGTTTTACCATTTCATCCACTATTTATTAAACCT
    AATAAAGATGAATTAGAAGTGATGTTTAATACAACAGTGAACTCAGACACAGATGTTATT
    AAATATGGTCGTTTGTTAGTTGATAAAGGTGCGCAATCTGTTATTGTCTCGCTTGGCGGT
    GATGGTGCTATTTATATTGATAAAGAAATCAGTATTAAAGCAGTTAATCCACAAGGGAAA
    GTGGTTAATACAGTTGGCTCTGGTGATAGTACAGTTGCAGGCATGGTGGCTGGAATTGCT
    TCAGGTTTAACGATTGAAAAAGCATTCCAACAAGCAGTCGCATGCGGTACTGCCACGGCA
    TTTGATGAGGACTTAGCAACACGGGACGCTATAGAAAAAATAAAATCACAAGTTACGATT
    AGCGTACTTGATGGGGAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    921

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA_RS03730 [old locus tag: SA0654 ]
  • symbol: SA_RS03730
  • description: 1-phosphofructokinase
  • length: 306
  • theoretical pI: 4.39114
  • theoretical MW: 32556.6
  • GRAVY: 0.0382353

Function[edit source | edit]

  • reaction:
    EC 2.7.1.144?  ExPASy
    Tagatose-6-phosphate kinaseATP + D-tagatose 6-phosphate = ADP + D-tagatose 1,6-bisphosphate
  • TIGRFAM:
    1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 354)
    hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 352.8)
    and 6 more
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidestagatose-6-phosphate kinase (TIGR01231; EC 2.7.1.144; HMM-score: 177.4)
    MetabolismEnergy metabolismSugarsribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 64.8)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesbifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 52.1)
    MetabolismEnergy metabolismSugars5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 42.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminehydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 36.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridoxinepyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 21.6)
  • TheSEED:
  • PFAM:
    Ribokinase (CL0118) PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 174.3)
    and 5 more
    Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 35)
    Carb_kinase; Carbohydrate kinase (PF01256; HMM-score: 23.1)
    HTH (CL0123) PuR_N; Bacterial purine repressor, N-terminal (PF09182; HMM-score: 14.4)
    NADP_Rossmann (CL0063) GRDA; Glycine reductase complex selenoprotein A (PF04723; HMM-score: 14.3)
    SIS (CL0067) DUF2529; Domain of unknown function (DUF2529) (PF10740; HMM-score: 11.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.126
    • Ymax_pos: 31
    • Cmax: 0.154
    • Cmax_pos: 31
    • Smax: 0.13
    • Smax_pos: 26
    • Smean: 0.092
    • D: 0.113
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446563391 NCBI
  • RefSeq: WP_000640737 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSRVLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEINAPGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVVDAEKELAESVLPFHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSLGGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACGTATAFDEDLATRDAIEKIKSQVTISVLDGE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA_RS03730 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]