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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS05595 [old locus tag: SA0987 ]
  • pan locus tag?: SAUPAN003376000
  • symbol: SA_RS05595
  • pan gene symbol?: rnhC
  • synonym:
  • product: ribonuclease HIII

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA_RS05595 [old locus tag: SA0987 ]
  • symbol: SA_RS05595
  • product: ribonuclease HIII
  • replicon: chromosome
  • strand: -
  • coordinates: 1118205..1119143
  • length: 939
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002745 (1118205..1119143) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCGAATATCGTTTTTAAATTGTCGGATAAAGACATAACGACATTAATGTCACGCATT
    ACTTTTGATACTGAGAATTTACCTCAAGGTATGAAAGCACGTGCAAAGTATCAAAATACA
    ACTGTAAATATTTACCAATCAGGTAAAGTCATGTTTCAAGGTAATCATGCAGAAGCTGTG
    TCTAAAGAACTTCTACCGCAGCATTCTCAATTGAATACTAATAAAACTAAAAAGAAAAAT
    ATGGCTAATTCTTCTCTTGAACAAACTTTAATGTATGATCAATTTAACTGTATTGGTAGT
    GATGAGGCTGGAAGTGGCGATTACTTTGGTCCTTTGACTGTGTGTGCTGCTTTTGTTACC
    AAAGAGCATGTTCCTATTTTAAAAACTTTGGGTGTAGATGATTCAAAAAAATTAACCGAT
    ACCAAAATTGTAGAACTTGCTGAGCAATTGGTTGCTTTCATCCCACATTCACTATTAACA
    CTACACAATGATAAATATAATATTCAGCAAGCAAAAGGCTGGACTCAAGTAAAAATGAAA
    GCTGTTCTTCATAATGAAGCCATCAAAAATGTACTTGAAAAAATTGATTCGTCTCAATTA
    GACTATATCGTCATTGATCAGTTCGCAAAACGCGAAGTGTATAGCCATTACGCATTATCC
    GATATACCACTTCCAAAGAAAACAAAATTTGAAACAAAAGGAGAATCGAAGTCATTAGCC
    ATTGCAGTGGCAAGCATAATTTCTAGATACGCATTTATTACATATATGGATCAAATATCA
    AAATATATTAATATGACGATTCCTAAAGGTGCCGGTGCTAAAGTCGATGTAATTGCCGCA
    AAAATTATAAAAAAATACGGTCTTTCACGTTTAGATACCATTTCAAAAAAGCATTTTAAA
    AATCGTGAAAAAGCCCAAAAAATTTTAAAGCCTCTTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    939

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA_RS05595 [old locus tag: SA0987 ]
  • symbol: SA_RS05595
  • description: ribonuclease HIII
  • length: 312
  • theoretical pI: 10.0243
  • theoretical MW: 35054.4
  • GRAVY: -0.322115

Function[edit source | edit]

  • reaction:
    EC 3.1.26.4?  ExPASy
    Ribonuclease HEndonucleolytic cleavage to 5'-phosphomonoester
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairribonuclease HIII (TIGR00716; EC 3.1.26.4; HMM-score: 226.7)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairribonuclease HII (TIGR00729; EC 3.1.26.4; HMM-score: 60.7)
    MetabolismEnergy metabolismElectron transportubiquinol-cytochrome c reductase, iron-sulfur subunit (TIGR01416; EC 1.10.2.2; HMM-score: 10.5)
  • TheSEED:
  • PFAM:
    RNase_H (CL0219) RNase_HII; Ribonuclease HII (PF01351; HMM-score: 157.2)
    TBP-like (CL0407) DUF3378; Domain of unknown function (DUF3378) (PF11858; HMM-score: 74)
    TBP; Transcription factor TFIID (or TATA-binding protein, TBP) (PF00352; HMM-score: 13.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+, Mn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.231
    • Ymax_pos: 34
    • Cmax: 0.291
    • Cmax_pos: 60
    • Smax: 0.422
    • Smax_pos: 31
    • Smean: 0.22
    • D: 0.227
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447207026 NCBI
  • RefSeq: WP_001284282 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MANIVFKLSDKDITTLMSRITFDTENLPQGMKARAKYQNTTVNIYQSGKVMFQGNHAEAVSKELLPQHSQLNTNKTKKKNMANSSLEQTLMYDQFNCIGSDEAGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVAFIPHSLLTLHNDKYNIQQAKGWTQVKMKAVLHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYALSDIPLPKKTKFETKGESKSLAIAVASIISRYAFITYMDQISKYINMTIPKGAGAKVDVIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA_RS05595 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]