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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS07145 [old locus tag: SA1260 ]
  • pan locus tag?: SAUPAN003859000
  • symbol: SA_RS07145
  • pan gene symbol?: thyA
  • synonym:
  • product: thymidylate synthase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS07145 [old locus tag: SA1260 ]
  • symbol: SA_RS07145
  • product: thymidylate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1432585..1433541
  • length: 957
  • essential: yes DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (1432585..1433541) NCBI
  • BioCyc: SA_RS07145 BioCyc
  • MicrobesOnline: see SA1260

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTGAATTCATTTGATGCAGCATATCACAGTCTTTGTGAAGAGGTTTTAGAAATAGGA
    AATACACGAAATGATCGCACAAATACAGGTACGATTTCGAAATTTGGTCATCAACTTCGC
    TTTGACTTATCTAAAGGATTTCCACTATTAACGACAAAGAAAGTTTCTTTTAAATTAGTA
    GCAACCGAATTATTATGGTTCATTAAAGGAGATACAAACATCCAATACTTATTAAAATAT
    AATAATAATATTTGGAACGAATGGGCTTTTGAAAATTATATAAAATCAGACGAGTATAAC
    GGTCCAGATATGACAGATTTCGGGCATCGTGCATTGAGTGATCCTGAATTTAACGAACAA
    TATAAAGAACAAATGAAACAATTTAAGCAACGTATTCTTGAAGATGATACTTTTGCGAAG
    CAATTCGGTGATTTAGGAAATGTTTATGGTAAACAATGGCGAGATTGGGTTGATAAAGAT
    GGTAATCATTTTGATCAACTTAAAACAGTAATTGAACAAATTAAGCATAATCCAGATTCA
    AGACGACACATCGTATCTGCATGGAATCCAACAGAAATTGATACAATGGCACTTCCGCCT
    TGTCATACCATGTTCCAGTTTTATGTCCAAGATGGTAAGTTAAGTTGCCAGTTATACCAA
    CGTAGCGCAGATATCTTTTTAGGTGTGCCATTTAATATCGCAAGCTACGCTTTATTGACA
    CACCTTATTGCCAAAGAATGTGGACTTGAAGTAGGAGAATTTGTGCATACATTTGGAGAT
    GCACATATTTATTCAAATCATATTGATGCGATTCAAACACAATTAGCACGTGAAAGCTTC
    AATCCTCCAACATTAAAAATTAACAGTGACAAGTCTATTTTCGACATAAATTATGAAGAT
    TTGGAAATTGTTGACTATGAATCACATCCAGCAATAAAAGCTCCAATAGCAGTGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    957

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS07145 [old locus tag: SA1260 ]
  • symbol: SA_RS07145
  • description: thymidylate synthase
  • length: 318
  • theoretical pI: 5.21628
  • theoretical MW: 36825.1
  • GRAVY: -0.487421

Function[edit | edit source]

  • reaction:
    EC 2.1.1.45?  ExPASy
    Thymidylate synthase 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism thymidylate synthase (TIGR03284; EC 2.1.1.45; HMM-score: 530)
    and 1 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism thymidylate synthase, methanogen type (TIGR03283; EC 2.1.1.-; HMM-score: 70.8)
  • TheSEED: see SA1260
  • PFAM:
    no clan defined Thymidylat_synt; Thymidylate synthase (PF00303; HMM-score: 393.8)
    and 3 more
    Nif11; Nif11 domain (PF07862; HMM-score: 16.8)
    RRM (CL0221) Tap-RNA_bind; Tap, RNA-binding (PF09162; HMM-score: 13.8)
    no clan defined LIAS_N; N-terminal domain of lipoyl synthase of Radical_SAM family (PF16881; HMM-score: 13.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008376
    • TAT(Tat/SPI): 0.001204
    • LIPO(Sec/SPII): 0.002181
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLSKGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYNGPDMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKDGNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA_RS01310acetyl-CoA acetyltransferase  [1] (data from MRSA252)
    SA_RS013153-hydroxyacyl-CoA dehydrogenase  [1] (data from MRSA252)
    SA_RS04895NAD(+) kinase  [1] (data from MRSA252)
    SA_RS05350pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SA_RS08560pyruvate kinase  [1] (data from MRSA252)

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.0 1.1 1.2 1.3 1.4 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]