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NCBI: 26-AUG-2013

⊟Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1260 [new locus tag: SA_RS07145 ]
  • pan locus tag?: SAUPAN003859000
  • symbol: thyA
  • pan gene symbol?: thyA
  • synonym:
  • product: thymidylate synthase

⊟Genome View[edit source | edit]

⊟Gene[edit source | edit]

⊟General[edit source | edit]

  • type: CDS
  • locus tag: SA1260 [new locus tag: SA_RS07145 ]
  • symbol: thyA
  • product: thymidylate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1432585..1433541
  • length: 957
  • essential: yes DEG other strains

⊟Accession numbers[edit source | edit]

⊟Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTGAATTCATTTGATGCAGCATATCACAGTCTTTGTGAAGAGGTTTTAGAAATAGGA
    AATACACGAAATGATCGCACAAATACAGGTACGATTTCGAAATTTGGTCATCAACTTCGC
    TTTGACTTATCTAAAGGATTTCCACTATTAACGACAAAGAAAGTTTCTTTTAAATTAGTA
    GCAACCGAATTATTATGGTTCATTAAAGGAGATACAAACATCCAATACTTATTAAAATAT
    AATAATAATATTTGGAACGAATGGGCTTTTGAAAATTATATAAAATCAGACGAGTATAAC
    GGTCCAGATATGACAGATTTCGGGCATCGTGCATTGAGTGATCCTGAATTTAACGAACAA
    TATAAAGAACAAATGAAACAATTTAAGCAACGTATTCTTGAAGATGATACTTTTGCGAAG
    CAATTCGGTGATTTAGGAAATGTTTATGGTAAACAATGGCGAGATTGGGTTGATAAAGAT
    GGTAATCATTTTGATCAACTTAAAACAGTAATTGAACAAATTAAGCATAATCCAGATTCA
    AGACGACACATCGTATCTGCATGGAATCCAACAGAAATTGATACAATGGCACTTCCGCCT
    TGTCATACCATGTTCCAGTTTTATGTCCAAGATGGTAAGTTAAGTTGCCAGTTATACCAA
    CGTAGCGCAGATATCTTTTTAGGTGTGCCATTTAATATCGCAAGCTACGCTTTATTGACA
    CACCTTATTGCCAAAGAATGTGGACTTGAAGTAGGAGAATTTGTGCATACATTTGGAGAT
    GCACATATTTATTCAAATCATATTGATGCGATTCAAACACAATTAGCACGTGAAAGCTTC
    AATCCTCCAACATTAAAAATTAACAGTGACAAGTCTATTTTCGACATAAATTATGAAGAT
    TTGGAAATTGTTGACTATGAATCACATCCAGCAATAAAAGCTCCAATAGCAGTGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    957

⊟Protein[edit source | edit]

⊟General[edit source | edit]

  • locus tag: SA1260 [new locus tag: SA_RS07145 ]
  • symbol: ThyA
  • description: thymidylate synthase
  • length: 318
  • theoretical pI: 5.21628
  • theoretical MW: 36825.1
  • GRAVY: -0.487421

⊟Function[edit source | edit]

  • reaction:
    EC 2.1.1.45?  ExPASy
    Thymidylate synthase5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotides2'-Deoxyribonucleotide metabolismthymidylate synthase (TIGR03284; EC 2.1.1.45; HMM-score: 530)
    and 1 more
    MetabolismPurines, pyrimidines, nucleosides, and nucleotides2'-Deoxyribonucleotide metabolismthymidylate synthase, methanogen type (TIGR03283; EC 2.1.1.-; HMM-score: 70.8)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsFolate and pterinesFolate Biosynthesis Thymidylate synthase (EC 2.1.1.45) 
    and 1 more
    Nucleosides and NucleotidesPyrimidinespyrimidine conversions Thymidylate synthase (EC 2.1.1.45) 
  • PFAM:
    no clan definedThymidylat_synt; Thymidylate synthase (PF00303; HMM-score: 393.8)
    and 3 more
    Nif11; Nif11 domain (PF07862; HMM-score: 16.8)
    RRM (CL0221) Tap-RNA_bind; Tap, RNA-binding (PF09162; HMM-score: 13.8)
    no clan definedLIAS_N; N-terminal domain of lipoyl synthase of Radical_SAM family (PF16881; HMM-score: 13.2)

⊟Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA0943-1(pdhA)pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SA0865(ppnK)inorganic polyphosphate/ATP-NAD kinase  [1] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [1] (data from MRSA252)
    SA0223hypothetical protein  [1] (data from MRSA252)
    SA0224hypothetical protein  [1] (data from MRSA252)
    SA0627hypothetical protein  [1] (data from MRSA252)

⊟Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.116
    • Ymax_pos: 26
    • Cmax: 0.105
    • Cmax_pos: 26
    • Smax: 0.173
    • Smax_pos: 18
    • Smean: 0.123
    • D: 0.118
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit source | edit]

⊟Protein sequence[edit source | edit]

  • MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLSKGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYNGPDMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKDGNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV

⊟Experimental data[edit source | edit]

  • experimentally validated: no data available

⊟Expression & Regulation[edit source | edit]

⊟Operon[edit source | edit]

⊟Regulation[edit source | edit]

  • sigma factor:
  • regulator:

⊟Transcription pattern[edit source | edit]

⊟Protein synthesis (provided by Aureolib)[edit source | edit]

⊟Protein stability[edit source | edit]

  • half-life: no data available

⊟Biological Material[edit source | edit]

⊟Mutants[edit source | edit]

⊟Expression vector[edit source | edit]

⊟lacZ fusion[edit source | edit]

⊟GFP fusion[edit source | edit]

⊟two-hybrid system[edit source | edit]

⊟FLAG-tag construct[edit source | edit]

⊟Antibody[edit source | edit]

⊟Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

⊟Literature[edit source | edit]

⊟References[edit source | edit]

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit source | edit]

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