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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS13880 [old locus tag: SA2420 ]
  • pan locus tag?: SAUPAN006349000
  • symbol: SA_RS13880
  • pan gene symbol?: phoB
  • synonym:
  • product: alkaline phosphatase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA_RS13880 [old locus tag: SA2420 ]
  • symbol: SA_RS13880
  • product: alkaline phosphatase
  • replicon: chromosome
  • strand: +
  • coordinates: 2715011..2716435
  • length: 1425
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002745 (2715011..2716435) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTTATCAGTTAAAAAAGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACA
    GTTTTCAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGT
    TTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATG
    GGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATTCGCATGCCAAAGAAATG
    ACCCCTACTGCTTTTGATTCATATTTAAAAGGCACAAATCGTACATATTCAAATGACCCA
    AAACAGAATATTACTGATTCTGCAGCTGGCGGAACAGCCTTTAGTTCTGGCCATAAGACA
    TATAATGGTGCCATCGGTGTTGACAGTAACAAGCAGAAAGTAAAGACAGTGCTTGAACGT
    GCAAAAGAAAAAGGTAAGTCGACTGGTCTTGTTTCAACAGCTGAATTAACAGATGCAACA
    CCAGCAGCATATGCTGCACACGTGACTTCTAGAGATGATAAAAACGAAATCGCTAAACAA
    TTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCT
    AAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGAC
    CTTGCCACGAACAGTAAGGATTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTT
    GCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGACGAACCATCATTAGCTGAT
    ATGCAACAAAGTGCACTTAGTAAGCTTGAGCGTAATAAAAAAGGTTTCTTTTTAATGGTC
    GAAGGTGCTTCTATAGATAAATCAGCGCATTCCAACGATATTACAGGCGTTATGTCTGAA
    ATGGAAGGTTTTGAAAAAGCATTCGATGACGCCATTCAATATGCTAAGAAACATAAAGAT
    ACACTTGTCGTTGCAACAGCAGATCACTCTACTGGTGGTCTAACAATTGGTAAAGATAAA
    GGATACGAATGGAATCCTCAACCGATTAAATCGATGAAACACTCTGGATCATATATGACA
    GAAAAAATGGCTAAAGGTGAAGACCCTGAAAAAGTTATTAATGAAGGTTATGGCTTTGAA
    TTTTCACAAGATGATATGAAAAAGGTTAAAAAAGAGAATAAAAAGTTGCAAAAATTACTT
    AAAAAAGAAAAAGACGAAAAATCTGCAGATGTAGAAAAACAAACAAAAGCCTTACAACAC
    GCAATTCAGAAGCCAGTAAATGATAAATCTTATACAGGGTGGACTTCCGATGGACATACA
    GGCGAGGATGTTAATACCTACGCTTACGGACCTAGATCAGAATCATTTAGTGGTAATATC
    GAAAATACAGAAAGCGCAAAAATAATATTTGATATATTCAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1425

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA_RS13880 [old locus tag: SA2420 ]
  • symbol: SA_RS13880
  • description: alkaline phosphatase
  • length: 474
  • theoretical pI: 9.5982
  • theoretical MW: 51920.9
  • GRAVY: -0.762025

Function[edit source | edit]

  • reaction:
    EC 3.1.3.1?  ExPASy
    Alkaline phosphataseA phosphate monoester + H2O = an alcohol + phosphate
  • TIGRFAM:
  • TheSEED:
  • PFAM:
    Alk_phosphatase (CL0088) Alk_phosphatase; Alkaline phosphatase (PF00245; HMM-score: 389)
    Metalloenzyme; Metalloenzyme superfamily (PF01676; HMM-score: 24.9)
    no clan definedDUF1889; Domain of unknown function (DUF1889) (PF08986; HMM-score: 14.8)
    TipAS; TipAS antibiotic-recognition domain (PF07739; HMM-score: 14.1)
    NADP_Rossmann (CL0063) Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 14.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide length 31 aa
    • Ymax: 0.832
    • Ymax_pos: 31
    • Cmax: 0.769
    • Cmax_pos: 31
    • Smax: 0.962
    • Smax_pos: 17
    • Smean: 0.871
    • D: 0.847
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446876499 NCBI
  • RefSeq: WP_000953755 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGMGPSFNTAYRYYKNDSHAKEMTPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDATPAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKDLSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLQKLLKKEKDEKSADVEKQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA_RS13880 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]