PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000159
• pan locus tag^?: SAUPAN001076000
• symbol: JSNZ_000159
• pan gene symbol^?: hptR
• synonym:
• product: response regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000159
• symbol: JSNZ_000159
• product: response regulator
• replicon: chromosome
• strand: -
• coordinates: 191665..192423
• length: 759
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  
  ATGTTTAAGGTAGTTATTTGTGATGATGAACGAATTATTCGAGAAGGATTAAAGCAAATC
  ATTCCCTGGGGAGACTATCATTTCAATACAATATACACTGCTAAAGATGGTGTTGAAGCA
  TTATCACTAATTCAACAACATCAGCCAGAATTAGTAATTACTGATATTCGTATGCCACGT
  AAAAATGGCGTCGATTTACTTAATGATATTGCGCATCTAGATTGTAATGTCATCATATTA
  TCGAGTTACGATGACTTTGAATATATGAAAGCCGGCATTCAACATCATGTTCTTGATTAT
  TTATTAAAACCAGTAGACCATGCACAATTAGAAGTTATTTTAGGAAGACTTGTCCGTACC
  TTATTAGAGCAACAATCACAGAACGGCCGTAGCTTAGCACCTTGTCATGATGCCTTCCAA
  CCACTATTAAAAGTAGAATACGATGATTATTATGTAAACCAGATTGTTGATCAAATTAAG
  CAATCTTATCAAACGAAAGTGACTGTTTCAGATTTAATTCGACATATCGATGTTAGTGAA
  TCGTATGCGATGCGAACATTTAAAGATCATGTTGGTATTACAATTGTCGATTATTTAAAT
  CGCTATCGTATTTTACAATCTCTGCAATTGTTAGATCGACATTACAAACACTATGAAATT
  GCAGACAAAGTAGGCTTTTCAGAGTATAAAATGTTTAGCTATCATTTTAAAAAATATTTA
  CAAATGTCTCCAAGTGATTATTGTAAGCAAGCAAAATAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  759
  

⊟Protein

⊟General

• locus tag: JSNZ_000159
• symbol: JSNZ_000159
• description: response regulator
• length: 252
• theoretical pI: 6.64604
• theoretical MW: 29636.8
• GRAVY: -0.324206

⊟Function

• TIGRFAM:
  YSIRK-targeted surface antigen transcriptional regulator (TIGR04094; HMM-score: 61.4)
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 55.9)
  Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 55.9)
  Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 55.9)
  Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 52.6)
  and 7 more
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 38.9)
  Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 34.5)
  Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 30.5)
  Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 30.5)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 20.4)
  proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 19.2)
  4-hydroxyphenylacetate catabolism regulatory protein HpaA (TIGR02297; HMM-score: 14.6)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 84.4)
  and 5 more
  HTH (CL0123) HTH_18; Helix-turn-helix domain (PF12833; HMM-score: 56.6)
  HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 45.7)
  DUF7347; Domain of unknown function (DUF7347) (PF24038; HMM-score: 14.9)
  no clan defined SecA_SW; SecA Wing and Scaffold domain (PF07516; HMM-score: 14)
  HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 11.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9902
  • Cytoplasmic Membrane Score: 0.0015
  • Cell wall & surface Score: 0.0007
  • Extracellular Score: 0.0076
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00146
  • TAT(Tat/SPI): 0.000078
  • LIPO(Sec/SPII): 0.000234
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLIQQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLLKPVDHAQLEVILGRLVRTLLEQQSQNGRSLAPCHDAFQPLLKVEYDDYYVNQIVDQIKQSYQTKVTVSDLIRHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHYEIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• Operon-mapper  : JSNZ_000159 < JSNZ_000160 < JSNZ_000161

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications

Joo Youn Park, Jong Wan Kim, Bo Youn Moon, Juyeun Lee, Ye Ji Fortin, Frank W Austin, Soo-Jin Yang, Keun Seok Seo
Characterization of a novel two-component regulatory system, HptRS, the regulator for the hexose phosphate transport system in Staphylococcus aureus.
Infect Immun: 2015, 83(4);1620-8
[PubMed:25644013] [WorldCat.org] [DOI] (I p)