PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0217 [new locus tag: SAUSA300_RS01140 ]
• pan locus tag^?: SAUPAN001076000
• symbol: SAUSA300_0217
• pan gene symbol^?: hptR
• synonym:
• product: AraC family DNA-binding response regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0217 [new locus tag: SAUSA300_RS01140 ]
• symbol: SAUSA300_0217
• product: AraC family DNA-binding response regulator
• replicon: chromosome
• strand: -
• coordinates: 256719..257477
• length: 759
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914472 NCBI
• RefSeq: YP_492931 NCBI
• BioCyc: see SAUSA300_RS01140
• MicrobesOnline: 1291732 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTTTAAGGTAGTTATTTGTGATGATGAACGAATTATTCGAGAAGGATTAAAGCAAATC
  ATTCCTTGGGGAGACTATCATTTCAATACAATATACACTGCTAAAGATGGTGTTGAAGCA
  TTATCACTAATTCAACAACATCAGCCAGAATTAGTAATTACTGATATTCGTATGCCACGT
  AAAAATGGCGTCGATTTACTCAATGATATTGCGCATCTAGATTGTAATGTCATCATATTA
  TCGAGTTACGATGACTTTGAATATATGAAAGCCGGCATTCAACATCATGTTCTTGATTAT
  TTATTAAAACCAGTAGACCATGCACAATTAGAAGTTATTTTAGGAAGACTTGTCCGTACC
  TTATTAGAGCAACAATCACAGAACGGCCGTAGCTTAGCATCTTGTCATGATGCCTTCCAA
  CCACTATTAAAAGTAGAATACGATGATTATTATGTAAACCAGATTGTGGATCAAATTAAG
  CAATCTTATCAAACGAAAGTGACTGTTTCAGATTTAATTCAACATATCGATGTTAGTGAA
  TCGTATGCGATGCGAACATTTAAAGATCATGTTGGTATTACAATTGTCGATTATTTAAAT
  CGCTATCGTATTTTACAATCTCTGCAATTGTTAGATCGACATTACAAACACTATGAAATT
  GCAGACAAAGTAGGCTTTTCAGAGTATAAAATGTTTAGCTATCATTTTAAAAAATATTTA
  CAAATGTCGCCAAGTGATTATTGTAAGCAAGCAAAATAG
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⊟Protein

⊟General

• locus tag: SAUSA300_0217 [new locus tag: SAUSA300_RS01140 ]
• symbol: SAUSA300_0217
• description: AraC family DNA-binding response regulator
• length: 252
• theoretical pI: 6.50679
• theoretical MW: 29598.7
• GRAVY: -0.317063

⊟Function

• TIGRFAM:
  YSIRK-targeted surface antigen transcriptional regulator (TIGR04094; HMM-score: 61.7)
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 56.2)
  Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 56.2)
  Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 56.2)
  Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 52.9)
  and 8 more
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 39.5)
  Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 36.3)
  Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 30.6)
  Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 30.6)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 20.5)
  4-hydroxyphenylacetate catabolism regulatory protein HpaA (TIGR02297; HMM-score: 15.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 13.1)
  RNA polymerase sigma-70 factor, Planctomycetaceae-specific subfamily 1 (TIGR02984; HMM-score: 12.5)
• TheSEED  :
  • DNA-binding response regulator, AraC family
• PFAM:
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 86.8)
  and 3 more
  HTH (CL0123) HTH_18; Helix-turn-helix domain (PF12833; HMM-score: 58.3)
  HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 48)
  PEP-carboxyk (CL0374) Hpr_kinase_C; HPr Serine kinase C-terminal domain (PF07475; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00146
  • TAT(Tat/SPI): 0.000078
  • LIPO(Sec/SPII): 0.000234
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161960 NCBI
• RefSeq: YP_492931 NCBI
• UniProt: Q2FK47 UniProt

⊟Protein sequence

• MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLIQQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLLKPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIKQSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHYEIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0217 < SAUSA300_0218 < SAUSA300_0219

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Joo Youn Park, Jong Wan Kim, Bo Youn Moon, Juyeun Lee, Ye Ji Fortin, Frank W Austin, Soo-Jin Yang, Keun Seok Seo
Characterization of a novel two-component regulatory system, HptRS, the regulator for the hexose phosphate transport system in Staphylococcus aureus.
Infect Immun: 2015, 83(4);1620-8
[PubMed:25644013] [WorldCat.org] [DOI] (I p)