PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0218 [new locus tag: SAUSA300_RS01145 ]
• pan locus tag^?: SAUPAN001077000
• symbol: SAUSA300_0218
• pan gene symbol^?: hptS
• synonym:
• product: sensor histidine kinase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0218 [new locus tag: SAUSA300_RS01145 ]
• symbol: SAUSA300_0218
• product: sensor histidine kinase family protein
• replicon: chromosome
• strand: -
• coordinates: 257470..259026
• length: 1557
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913983 NCBI
• RefSeq: YP_492932 NCBI
• BioCyc: see SAUSA300_RS01145
• MicrobesOnline: 1291733 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  
  ATGACGGCATACAAACCTTATAGACATCAATTAAGACGATCGTTATTTGCGTCTACTATT
  TTTCCAGTATTTTTAGTCATTATTATTGGACTAGTAAGCTTCTATGCCATCTATATTTGG
  ATTGAGCATCGTACGATTCATCAACATGTTGATGAAAGTCAGTCATCTTTACATCATACT
  GAAAAGCAGATTCAGACATTTATTACACAGCATAATAATTCGTTTCAAGAGCTCGATTTA
  ACAAATCATCATGATGTGACCGCTACAAAACGTGAATTATTAAAATTAATACACCAACAG
  CCCGCAACACTTTATTATGAATTATCTGGACCAAATCAGTTTATAACCAATAATTATGAA
  CATTTAAACACTAAAAATATGTATTTATTTTCAACGCATCAGCTTAAATTCAAAAATAGT
  ACATATATGCTTAAAATTTATATGGCTAACACCCCACGTTTAAGTGAAATTAAGAAAGAT
  AACCGACAATTCGCACTTATCGTTGATCAATATGACAATATTTTATACGCCAACGATGAC
  CGTTTTACTATAGGTGAAAAGTATAGACCACAACAATTCGGATTTATGAATGAATCTGTA
  AAATTAAACCATGCTGACCATCGTCTCATTATTTATAAAGATATACATGAAAACATTGAA
  GATGGTATCACGTTGTTAATTGTTATGGCCGTAGTTCTTGTTTTACTAGTAATATTTGGT
  TTCATTAGCGCAGATAATATGGCTAAACGACAAACAAAAGATATCGAAACGATTATTCAA
  AAAATTTACTATGCCAAAAATCGTCATCTAGGTACATATACGCCTTTAAAAAACAACAGT
  GAACTAGAAGAAATTAATAATTATATTTACGATTTATTTGAATCAAATGAGCAACTGATC
  CATTCTATCGAGCATACAGAACGTCGCTTGCGTGATATTCAGTTAAAGGAAATAGAACGC
  CAATTCCAGCCTCATTTCCTATTCAATACGATGCAAACAATACAATATTTAATTACGTTA
  TCTCCGAAATTAGCACAAACTGTGGTCCAACAATTATCGCAAATGTTACGTTACTCATTA
  CGTACAAACTCACATACAGTGGAATTAAACGAAGAGTTGAACTACATTGAACAGTATGTT
  GCAATACAAAACATCCGTTTTGATGACATGATTAAGCTCCATATTGAAAGTTCTGAAGAA
  GCGCGTCACCAGACAATTGGTAAAATGATGCTTCAGCCACTCATAGAAAATGCTATTAAA
  CATGGTAGAGATACTGAGTCATTGGACATTACTATACGCCTGACATTGGCACGTCAGAAT
  TTACATGTTTTAGTTTGCGACAATGGTATCGGGATGTCGTCATCTCGCCTGCAATATGTA
  CGCCAATCTCTTAATAATGATGTATTTGATACTAAGCATTTGGGATTAAATCATTTACAC
  AATAAAGCAATGATTCAATATGGTTCACATGCACGACTCCATATTTTTTCAAAGCGTAAT
  CAAGGTACGTTAATTTGTTATAAAATACCACTTTCAAGGGGGAATGTAGATGTTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1557
  

⊟Protein

⊟General

• locus tag: SAUSA300_0218 [new locus tag: SAUSA300_RS01145 ]
• symbol: SAUSA300_0218
• description: sensor histidine kinase family protein
• length: 518
• theoretical pI: 8.26122
• theoretical MW: 61046.4
• GRAVY: -0.383591

⊟Function

• reaction:
  EC 2.7.13.3?  ExPASy
  Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  EC 2.7.3.-?  ExPASy
• TIGRFAM:
  Cellular processes Cellular processes Sporulation and germination sporulation integral membrane protein YtvI (TIGR02872; HMM-score: 16.6)
  Cellular processes Cellular processes Sporulation and germination anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 15.4)
  Signal transduction Regulatory functions Protein interactions anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 15.4)
  Signal transduction Regulatory functions DNA interactions transcriptional regulator, Spx/MgsR family (TIGR01617; HMM-score: 13.9)
  and 2 more
  Cellular processes Cellular processes Sporulation and germination stage II sporulation protein M (TIGR02831; HMM-score: 12.6)
  Cellular processes Cellular processes Cell division cell division protein FtsL (TIGR02209; HMM-score: 12.4)
• TheSEED  :
  • Two-component sensor histidine kinase
• PFAM:
  no clan defined His_kinase; Histidine kinase (PF06580; HMM-score: 76)
  and 10 more
  His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 25.8)
  no clan defined SIT; SHP2-interacting transmembrane adaptor protein, SIT (PF15330; HMM-score: 16.3)
  His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 14.5)
  no clan defined DUF2730; Protein of unknown function (DUF2730) (PF10805; HMM-score: 14.2)
  Tetraspannin (CL0347) Tetraspannin; Tetraspanin family (PF00335; HMM-score: 14.1)
  BPD_transp_1 (CL0404) FtsX; FtsX-like permease family (PF02687; HMM-score: 13.9)
  no clan defined Spore_III_AB; Stage III sporulation protein AB (spore_III_AB) (PF09548; HMM-score: 13.7)
  Phage_barrel (CL0504) Phage-tail_3; Putative phage tail protein (PF13550; HMM-score: 13.1)
  no clan defined DUF4477; Domain of unknown function (DUF4477) (PF14780; HMM-score: 9.5)
  STAT_int; STAT protein, protein interaction domain (PF02865; HMM-score: 7.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 9.99
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 2
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001519
  • TAT(Tat/SPI): 0.000272
  • LIPO(Sec/SPII): 0.001285
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 87160659 NCBI
• RefSeq: YP_492932 NCBI
• UniProt: Q2FK46 UniProt

⊟Protein sequence

• MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHRTIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPATLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKDNRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHENIEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPLKNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTIQYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIKLHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGIGMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYKIPLSRGNVDV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0217 < SAUSA300_0218 < SAUSA300_0219

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications