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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0202 [new locus tag: SACOL_RS01030 ]
- pan locus tag?: SAUPAN001077000
- symbol: SACOL0202
- pan gene symbol?: hptS
- synonym:
- product: sensor histidine kinase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0202 [new locus tag: SACOL_RS01030 ]
- symbol: SACOL0202
- product: sensor histidine kinase
- replicon: chromosome
- strand: -
- coordinates: 236712..238268
- length: 1557
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236772 NCBI
- RefSeq: YP_185101 NCBI
- BioCyc: see SACOL_RS01030
- MicrobesOnline: 911679 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1501ATGACGGCATACAAACCTTATAGACATCAATTAAGACGATCGTTATTTGCGTCTACTATT
TTTCCAGTATTTTTAGTCATTATTATTGGACTAGTAAGCTTCTATGCCATCTATATTTGG
ATTGAGCATCGTACGATTCATCAACATGTTGATGAAAGTCAGTCATCTTTACATCATACT
GAAAAGCAGATTCAGACATTTATTACACAGCATAATAATTCGTTTCAAGAGCTCGATTTA
ACAAATCATCATGATGTGACCGCTACAAAACGTGAATTATTAAAATTAATACACCAACAG
CCCGCAACACTTTATTATGAATTATCTGGACCAAATCAGTTTATAACCAATAATTATGAA
CATTTAAACACTAAAAATATGTATTTATTTTCAACGCATCAGCTTAAATTCAAAAATAGT
ACATATATGCTTAAAATTTATATGGCTAACACCCCACGTTTAAGTGAAATTAAGAAAGAT
AACCGACAATTCGCACTTATCGTTGATCAATATGACAATATTTTATACGCCAACGATGAC
CGTTTTACTATAGGTGAAAAGTATAGACCACAACAATTCGGATTTATGAATGAATCTGTA
AAATTAAACCATGCTGACCATCGTCTCATTATTTATAAAGATATACATGAAAACATTGAA
GATGGTATCACGTTGTTAATTGTTATGGCCGTAGTTCTTGTTTTACTAGTAATATTTGGT
TTCATTAGCGCAGATAATATGGCTAAACGACAAACAAAAGATATCGAAACGATTATTCAA
AAAATTTACTATGCCAAAAATCGTCATCTAGGTACATATACGCCTTTAAAAAACAACAGT
GAACTAGAAGAAATTAATAATTATATTTACGATTTATTTGAATCAAATGAGCAACTGATC
CATTCTATCGAGCATACAGAACGTCGCTTGCGTGATATTCAGTTAAAGGAAATAGAACGC
CAATTCCAGCCTCATTTCCTATTCAATACGATGCAAACAATACAATATTTAATTACGTTA
TCTCCGAAATTAGCACAAACTGTGGTCCAACAATTATCGCAAATGTTACGTTACTCATTA
CGTACAAACTCACATACAGTGGAATTAAACGAAGAGTTGAACTACATTGAACAGTATGTT
GCAATACAAAACATCCGTTTTGATGACATGATTAAGCTCCATATTGAAAGTTCTGAAGAA
GCGCGTCACCAGACAATTGGTAAAATGATGCTTCAGCCACTCATAGAAAATGCTATTAAA
CATGGTAGAGATACTGAGTCATTGGACATTACTATACGCCTGACATTGGCACGTCAGAAT
TTACATGTTTTAGTTTGCGACAATGGTATCGGGATGTCGTCATCTCGCCTGCAATATGTA
CGCCAATCTCTTAATAATGATGTATTTGATACTAAGCATTTGGGATTAAATCATTTACAC
AATAAAGCAATGATTCAATATGGTTCACATGCACGACTCCATATTTTTTCAAAGCGTAAT
CAAGGTACGTTAATTTGTTATAAAATACCACTTTCAAGGGGGAATGTAGATGTTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0202 [new locus tag: SACOL_RS01030 ]
- symbol: SACOL0202
- description: sensor histidine kinase
- length: 518
- theoretical pI: 8.26122
- theoretical MW: 61046.4
- GRAVY: -0.383591
⊟Function[edit | edit source]
- reaction: EC 2.7.13.3? ExPASyHistidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
- TIGRFAM: Cellular processes Sporulation and germination sporulation integral membrane protein YtvI (TIGR02872; HMM-score: 16.6)Cellular processes Sporulation and germination anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 15.4)Regulatory functions Protein interactions anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 15.4)Regulatory functions DNA interactions transcriptional regulator, Spx/MgsR family (TIGR01617; HMM-score: 13.9)and 2 moreCellular processes Sporulation and germination stage II sporulation protein M (TIGR02831; HMM-score: 12.6)Cellular processes Cell division cell division protein FtsL (TIGR02209; HMM-score: 12.4)
- TheSEED :
- Two-component sensor histidine kinase
- PFAM: no clan defined His_kinase; Histidine kinase (PF06580; HMM-score: 76)and 10 moreHis_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 25.8)no clan defined SIT; SHP2-interacting transmembrane adaptor protein, SIT (PF15330; HMM-score: 16.3)His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 14.5)no clan defined DUF2730; Protein of unknown function (DUF2730) (PF10805; HMM-score: 14.2)Tetraspannin (CL0347) Tetraspannin; Tetraspanin family (PF00335; HMM-score: 14.1)BPD_transp_1 (CL0404) FtsX; FtsX-like permease family (PF02687; HMM-score: 13.9)no clan defined Spore_III_AB; Stage III sporulation protein AB (spore_III_AB) (PF09548; HMM-score: 13.7)Phage_barrel (CL0504) Phage-tail_3; Putative phage tail protein (PF13550; HMM-score: 13.1)no clan defined DUF4477; Domain of unknown function (DUF4477) (PF14780; HMM-score: 9.5)STAT_int; STAT protein, protein interaction domain (PF02865; HMM-score: 7.1)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0.01
- Cytoplasmic Membrane Score: 9.99
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 2
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -0.5
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001519
- TAT(Tat/SPI): 0.000272
- LIPO(Sec/SPII): 0.001285
- predicted transmembrane helices (TMHMM): 2
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHRTIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPATLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKDNRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHENIEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPLKNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTIQYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIKLHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGIGMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYKIPLSRGNVDV
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Integral membrane [1] [2]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit | edit source]
Stephan Fuchs, Jan Pané-Farré, Christian Kohler, Michael Hecker, Susanne Engelmann
Anaerobic gene expression in Staphylococcus aureus.
J Bacteriol: 2007, 189(11);4275-89
[PubMed:17384184] [WorldCat.org] [DOI] (P p)