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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0203 [new locus tag: SACOL_RS01035 ]
  • pan locus tag?: SAUPAN001079000
  • symbol: SACOL0203
  • pan gene symbol?: hptA
  • synonym:
  • product: iron compound ABC transporter iron compound-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0203 [new locus tag: SACOL_RS01035 ]
  • symbol: SACOL0203
  • product: iron compound ABC transporter iron compound-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 238265..239233
  • length: 969
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAATCAAAAATTTATATCTTGCTATTATTTCTCATTTTTTTATCAGCATGCGCTAAT
    ACGCGTCACTCTGAATCCGATAAAAATGTATTAACAGTTTATTCTCCGTATCAATCAAAC
    TTGATTCGTCCAATTTTAAATGAATTTGAAAAACAAGAGCATGTCAAAATTGAAATTAAA
    CACGGATCTACTCAAGTACTGCTTTCAAACTTGCATAACGAAGATTTTTCGGAGCGTGGT
    GATGTCTTTATGGGTGGTGTGTTGTCAGAAACAATTGATCATCCAGAAGATTTTGTTCCC
    TATCAAGATACATCTGTAACACAGCAATTAGAGGATTATCGCTCGAACAATAAATATGTT
    ACTAGTTTTCTATTAATGCCAACAGTTATAGTAGTGAATTCAGATTTACAAGGAGATATT
    AAGATTCGAGGTTATCAAGATTTATTACAACCTATACTTAAAGGTAAAATTGCGTACTCA
    AATCCAAATACAACAACGACAGGCTATCAACATATGCGTGCTATTTATAGCATGCATCAT
    CGAGTAAGTGATGTGCATCAATTCCAAAACCATGCGATGCAACTGTCAAAGACGTCTAAA
    GTCATTGAAGATGTTGCAAAAGGTAAATATTACGCAGGTCTAAGCTACGAACAAGATGCA
    CGCACATGGAAAAACAAAGGTTATCCTGTATCAATCGTTTATCCAATTGAAGGAACAATG
    TTAAATGTTGATGGTATTGCTTTAGTTAAAAATGCACACCCTCATCCTAAGCGTAAAAAG
    TTAGTGCAATATTTAACAAGCCGCTCAGTACAACAACGATTAGTTGCAGAGTTCGATGCC
    AAGTCAATTCGAAAGGATGTTAGTGAACAAAGCGATCAGTCTATCGAAAATCTTAAGAAC
    ATACCTTTAATACCGAAATCTAAATTACCGGATATTCCACATCATAAATTTTTGGAGATG
    ATTCAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0203 [new locus tag: SACOL_RS01035 ]
  • symbol: SACOL0203
  • description: iron compound ABC transporter iron compound-binding protein
  • length: 322
  • theoretical pI: 8.99606
  • theoretical MW: 36931.9
  • GRAVY: -0.455279

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, periplasmic 2-aminoethylphosphonate-binding protein (TIGR03261; HMM-score: 88.7)
    and 2 more
    2-aminoethylphosphonate ABC transporter, 2-aminoethylphosphonate binding protein (TIGR03227; HMM-score: 40.9)
    Metabolism Transport and binding proteins Other ABC transporter periplasmic binding protein, thiB subfamily (TIGR01254; HMM-score: 27.2)
  • TheSEED  :
    • Ferric iron ABC transporter, iron-binding protein
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Iron acquisition in Streptococcus  Ferric iron ABC transporter, iron-binding protein
  • PFAM:
    PBP (CL0177) SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 74.7)
    SBP_bac_6; Bacterial extracellular solute-binding protein (PF13343; HMM-score: 62.9)
    and 3 more
    SBP_bac_8; Bacterial extracellular solute-binding protein (PF13416; HMM-score: 38.7)
    SBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 23.1)
    LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: -0.33
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: FLSACAN
  • SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
    • SP(Sec/SPI): 0.001198
    • TAT(Tat/SPI): 0.000118
    • LIPO(Sec/SPII): 0.998256
    • Cleavage Site: CS pos: 17-18. LSA-CA. Pr: 0.9990
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRPILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQDTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYSNPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQDARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVAEFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Lipoprotein [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Joo Youn Park, Jong Wan Kim, Bo Youn Moon, Juyeun Lee, Ye Ji Fortin, Frank W Austin, Soo-Jin Yang, Keun Seok Seo
Characterization of a novel two-component regulatory system, HptRS, the regulator for the hexose phosphate transport system in Staphylococcus aureus.
Infect Immun: 2015, 83(4);1620-8
[PubMed:25644013] [WorldCat.org] [DOI] (I p)

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