PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0217 [new locus tag: SA_RS01270 ]
• pan locus tag^?: SAUPAN001079000
• symbol: SA0217
• pan gene symbol^?: hptA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0217 [new locus tag: SA_RS01270 ]
• symbol: SA0217
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 257310..258278
• length: 969
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1122994 NCBI
• RefSeq: NP_373461 NCBI
• BioCyc: see SA_RS01270
• MicrobesOnline: 102487 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  481
  541
  601
  661
  721
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  841
  901
  961
  
  ATGAAATCAAAAATTTATATCTTGCTATTATTTCTCATTTTTTTATCATCATGCGCTAAT
  ACGCGTCACTCTGAATCAGATAAAAATGTATTAACAGTTTATTCTCCGTATCAATCAAAC
  TTGATTCGTCCAATTTTAAATGAATTTGAAAAACAAGAGCATGTCAAAATTGAAATCAAA
  CATGGATCTACTCAAGTACTGCTTTCAAACTTGCATAACGAAGATTTTTCGGAGCGTGGT
  GACGTCTTTATGGGTGGTGTGTTATCAGAAACAATTGATCATCCAGAAGATTTTGTTCCC
  TATCAAGATACATCTGTAACACAGCAATTAGAGGATTATCGCTCGAACAATAAATATGTT
  ACTAGTTTTCTATTAATGCCAACAGTTATAGTAGTGAATTCAGATTTACAAGGAGATATT
  AAGATTCGAGGTTATCAAGATTTATTACAACCTATACTTAAAGGTAAAATTGCGTACTCA
  AATCCAAATACAACAACGACAGGCTATCAACATATGCGCGCTATTTATAGCATGCATCAT
  CGAGTAAGTGATGTGCATCAATTCCAAAACCATGCGATGCAACTGTCAAAGACGTCTAAA
  GTCATTGAAGATGTTGCAAAAGGTAAATATTACGCAGGTCTAAGCTACGAACAAGATGCA
  CGCACATGGAAAAACAAAGGTTATCCTGTATCAATCGTTTATCCAATTGAAGGAACAATG
  TTAAATGTTGATGGTATTGCTTTAGTTAAAAATGCACACCCTCATCCTAAGCGTAAAAAG
  TTAGTGCAATATTTAACAAGCCGCTCAGTACAACAACGATTAGTTGCAGAGTTCGATGCC
  AAGTCAATTCGAAAGGATGTTAGTGAACAAAGCGATCAGTCTATCGAAAATCTTAAGAAC
  ATACCTTTAATACCGAAATCTAAATTACCGGACATTCCACATCATAAATTTTTGGAGATG
  ATTCAATGA
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⊟Protein

⊟General

• locus tag: SA0217 [new locus tag: SA_RS01270 ]
• symbol: SA0217
• description: hypothetical protein
• length: 322
• theoretical pI: 8.99606
• theoretical MW: 36947.9
• GRAVY: -0.463354

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, periplasmic 2-aminoethylphosphonate-binding protein (TIGR03261; HMM-score: 88.1)
  and 2 more
  2-aminoethylphosphonate ABC transporter, 2-aminoethylphosphonate binding protein (TIGR03227; HMM-score: 40.7)
  Metabolism Transport and binding proteins Other ABC transporter periplasmic binding protein, thiB subfamily (TIGR01254; HMM-score: 27.2)
• TheSEED  :
  • Ferric iron ABC transporter, iron-binding protein
  Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Iron acquisition in Streptococcus  Ferric iron ABC transporter, iron-binding protein
• PFAM:
  PBP (CL0177) SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 74.7)
  SBP_bac_6; Bacterial extracellular solute-binding protein (PF13343; HMM-score: 62.9)
  and 2 more
  SBP_bac_8; Bacterial extracellular solute-binding protein (PF13416; HMM-score: 38.7)
  SBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 23.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 0
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 5
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
  • SP(Sec/SPI): 0.00128
  • TAT(Tat/SPI): 0.000126
  • LIPO(Sec/SPII): 0.998134
  • Cleavage Site: CS pos: 17-18. LSS-CA. Pr: 0.9989
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15925928 NCBI
• RefSeq: NP_373461 NCBI
• UniProt: A0A0H3JSM6 UniProt

⊟Protein sequence

• MKSKIYILLLFLIFLSSCANTRHSESDKNVLTVYSPYQSNLIRPILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQDTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYSNPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQDARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVAEFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0215 < SA0216 < SA0217

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Joo Youn Park, Jong Wan Kim, Bo Youn Moon, Juyeun Lee, Ye Ji Fortin, Frank W Austin, Soo-Jin Yang, Keun Seok Seo
Characterization of a novel two-component regulatory system, HptRS, the regulator for the hexose phosphate transport system in Staphylococcus aureus.
Infect Immun: 2015, 83(4);1620-8
[PubMed:25644013] [WorldCat.org] [DOI] (I p)