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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

organism: Staphylococcus aureus JSNZ
locus tag: JSNZ_001680
pan locus tag^?: SAUPAN004320000
symbol: JSNZ_001680
pan gene symbol^?: citZ
synonym:
product: citrate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: JSNZ_001680
symbol: JSNZ_001680
product: citrate synthase
replicon: chromosome
strand: -
coordinates: 1717239..1718360
length: 1122
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID:
RefSeq:
BioCyc:
MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCAGAATTACAAAGAGGTTTAGAAGGGGTTATCGCAGCGGAGACTAAAATAAGTTCA
ATTATTGAAAGTCAATTGACTTATGCCGGCTATGATATTGATGATCTAGCTGAAAATGCG
CAATTTGAAGAAGTTATTTTCCTATTATGGAACTATAGATTGCCAAACGAAGAAGAGCTT
GCTCATCTAAAAGGGAAATTAAATCAATACATGACATTAAATCCTCGTGTGTATACACAT
TTTGAGGAGTATGTTACAGATCACGTGCATCCAATGACAGCATTACGTACGTCATTATCA
TATATTGCACATTTCGATCCTGATGCTGAAAATGAATCAGATGAAAATCGTTATGAAAGA
GCAATGCGTATACAGGCTAAAGTAGCATCATTAGTTACAGCGTTTGCTCGAGTAAGACAA
GATAAAGAACCACTTAAGCCTAATCCTGACTTAAGTTATGCGGCAAACTTCCTATATATG
TTACGTGGGGAATTACCAACAGATATAGAAGTAGAAGCCTTCAATAAAGCACTTATTTTA
CACGCTGATCATGAGTTGAACGCATCTGCATTTACAGCACGTTGTGCGGTATCATCATTG
TCAGATATGTACTCAGGTATTGTAGCAGCTGTAGGTTCTCTGAAAGGGCCATTACATGGT
GGTGCAAACGAACAAGTTATGACGATGTTATCTGAGATTGGGTCAATTGAAAATGTTGAT
GCTTACTTAGATGAAAAATTTGCTAATAAAGATAAAGTAATGGGCTTCGGTCATCGTGTA
TATAAAGATGGTGATCCTAGAGCGAAATATTTAAGAGAAATGAGCCGTCAAATTACGAAA
GACGCTGGTCGTGAAGAATTATTTGAAATGTCAGTGAAAATGGAAAAACGTATGGCAGAA
GAAAAAGGATTAATTCCTAATGTTGATTTTTATAGTGCGAGTGTTTATCACTGTATGGAA
ATACCTCATGACTTATTCACGCCAATCTTTGCTGTAAGTCGTTCTGCAGGATGGATTGCT
CATATTTTAGAACAATATAAAGATAATAGAATTATGCGTCCTAGAGCGAAATATATTGGC
GAAACGAATCGTAAGTATATCCCGCTTGAAGAAAGAAAATAA
60
120
180
240
300
360
420
480
540
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840
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1020
1080
1122

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: JSNZ_001680
symbol: JSNZ_001680
description: citrate synthase
length: 373
theoretical pI: 5.33842
theoretical MW: 42593.1
GRAVY: -0.416086

⊟Function[edit | edit source]

TIGRFAM:
2-methylcitrate synthase/citrate synthase II (TIGR01800; HMM-score: 490.6)
and 2 more
Metabolism Energy metabolism TCA cycle citrate (Si)-synthase (TIGR01798; EC 2.3.3.1; HMM-score: 263.9)
citrate (Si)-synthase, eukaryotic (TIGR01793; EC 2.3.3.1; HMM-score: 83.6)
TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
PFAM:
no clan defined Citrate_synt; Citrate synthase, C-terminal domain (PF00285; HMM-score: 448.5)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9063
- Cytoplasmic Membrane Score: 0.0086
- Cell wall & surface Score: 0.0002
- Extracellular Score: 0.0849
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002539
- TAT(Tat/SPI): 0.000292
- LIPO(Sec/SPII): 0.000332
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI:
RefSeq:
UniProt:

⊟Protein sequence[edit | edit source]

MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEEVIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYIAHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYMLRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPLHGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSRQITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSRSAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell: data available for COL
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

Operon-mapper ^[1] : icd < JSNZ_001680

⊟Regulation[edit | edit source]

regulator: CcpA regulon
CcpA (TF) important in Carbon catabolism; regulation predicted or transferred from N315 and NCTC 8325 ^[2]

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: data available for COL

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
Bioinformatics: 2018, 34(23);4118-4120
[PubMed:29931111] [WorldCat.org] [DOI] (I p)
↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
Curr Res Microb Sci: 2025, 9;100489
[PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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