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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002298
  • pan locus tag?: SAUPAN005817000
  • symbol: hutI
  • pan gene symbol?: hutI
  • synonym:
  • product: imidazolonepropionase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002298
  • symbol: hutI
  • product: imidazolonepropionase
  • replicon: chromosome
  • strand: -
  • coordinates: 2294882..2296120
  • length: 1239
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATGATTTAATAATTAATCATATAGCAGAATTAATTTTACCGAAATCAACAGATAAA
    CCTTTGAAGGGTAAGGAATTAGATGAATTAAATGTTGTGAAAAATGGTACAGTTGTCATT
    AAAGATGGCAAAATTGTATATGCTGGACAACATACAGATGACTATGATGCGACTGAGACG
    ATAGATGCTAGTGGAAAAGTAGTTTCTCCGGCATTAGTAGATGCTCATACCCATTTAACA
    TTTGGTGGATCTCGAGAACATGAGATGTCATTAAAACGTCAAGGTAAGTCTTACCTTGAA
    ATATTAGAAATGGGTGGTGGCATATTATCTACCGTTAATGCTACTAGAGAAACATCCGAA
    GATGACTTGTTTAAAAAAGCAGAGCATGATTTGCTTACTATGATTAAACACGGCGTGCTT
    GCAGTTGAAAGTAAGAGTGGTTATGGATTAGATAGAGAAAATGAACTGAAGCAATTGAAA
    GTGTCTAATCGTTTAGCTGAGAAATATGATTTAGACATGAAACATACTTTCCTAGGGCCT
    CATGCTGTACCTAAAGAGGCAAGTTCAAATGAGGCATTTTTAGAAGAAATGATTGCGTTA
    CTTCCGGAAGTAAAACAATATGCAGACTTTGCGGATATTTTCTGTGAAACAGGTGTATTT
    ACAATAGAACAATCGCAACATTATATGCAAAAAGCCAAAGAAGCAGGTTTTAAAGTGAAA
    ATACATGCGGATGAAATTGATCCGTTAGGCGGACTGGAATTAGCAATTGATGAACAAGCA
    ATATCAGCAGACCACTTAGTAGCTTCTAGTGATAAAGGAAAAGAAAAGTTGCGAAATAGT
    GATACTGTAGCTGTCCTCTTACCTGCAACGACGTTCTATTTAGGTAAAGAAGATTATGCA
    GATGCAAGAGGCATGCTTGACAATAACGGTGCGATTGCATTAGCAACTGATTATAACCCT
    GGTAGTAGTGTCACAAACAACTTACAACTTGTTATGGCGATTGCAGCCTTGAAATTAAAG
    CTATCACCTAATGAAGTTTGGAATGCTGTTACGGTCAATGCTGCTAAAGCAATAGACATT
    AACGCGGGTACAATTAACACAGGTGATAAGGCTAATTTAGTAATTTGGGATGCACCAAAT
    CATGAATATATTCCATATCATTTTGGTATCAATCACGCAGAAAAAGTTATTAAAGACGGT
    AAGGTCATCGTAGATAACACAGTATCTTTTAAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1239

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002298
  • symbol: HutI
  • description: imidazolonepropionase
  • length: 412
  • theoretical pI: 5.03086
  • theoretical MW: 45037.7
  • GRAVY: -0.264078

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.2.7?  ExPASy
    Imidazolonepropionase (S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines imidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 435.6)
    and 11 more
    Metabolism Energy metabolism Other phosphonate metabolism protein PhnM (TIGR02318; HMM-score: 45.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 38.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other guanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 37.6)
    allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 37.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 36)
    dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 33.6)
    Metabolism Central intermediary metabolism Amino sugars N-acetylglucosamine-6-phosphate deacetylase (TIGR00221; EC 3.5.1.25; HMM-score: 33.5)
    formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 33.3)
    Hypothetical proteins Conserved putative amidohydrolase, EF_0837/AHA_3915 family (TIGR03583; HMM-score: 32.2)
    putative selenium metabolism protein SsnA (TIGR03314; HMM-score: 26.1)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides beta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 18.8)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 71.4)
    Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 61.9)
    and 3 more
    Phage_TACs (CL0567) Phage_TAC_6; Phage tail assembly chaperone protein, TAC (PF09550; HMM-score: 15.7)
    no clan defined DUF6799; Domain of unknown function (DUF6799) (PF20606; HMM-score: 14.3)
    TIM_barrel (CL0036) BsNagA_N; BsNagA composite domain (PF22644; HMM-score: 11)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9931
    • Cytoplasmic Membrane Score: 0.0009
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0057
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006451
    • TAT(Tat/SPI): 0.000242
    • LIPO(Sec/SPII): 0.000745
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNDLIINHIAELILPKSTDKPLKGKELDELNVVKNGTVVIKDGKIVYAGQHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEILEMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLKVSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETGVFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEKLRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAIAALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINHAEKVIKDGKVIVDNTVSFKA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: HutR* (activation) regulon, CcpA regulon
    HutR*(TF)important in Histidine utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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