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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002442
  • pan locus tag?: SAUPAN006027000
  • symbol: cntA
  • pan gene symbol?: cntA
  • synonym:
  • product: staphylopine-dependent metal ABC transporter substrate-binding protein CntA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002442
  • symbol: cntA
  • product: staphylopine-dependent metal ABC transporter substrate-binding protein CntA
  • replicon: chromosome
  • strand: -
  • coordinates: 2442782..2444380
  • length: 1599
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGAGAAAACTAACTAAAATGAGTGCAATGTTACTTGCATCAGGGCTAATTTTAACCGGT
    TGTGGCGGTAATAAAGGTTTAGAGGAGAAAAAAGAAAACAAGCAATTAACGTATACGACG
    GTTAAAGATATCGGTGATATGAATCCGCATGTTTACGGTGGATCGATGTCTGCTGAAAGT
    ATGATATACGAGCCGCTTGTACGTAACACGAAAGATGGAATTAAGCCTTTACTAGCTAAA
    AAATGGGATGTGTCTGAAGATGGGAAGACATATACATTCCATTTGAGAGATGACGTTAAA
    TTCCATGATGGTACACCATTTGATGCTGACGCAGTTAAGAAAAATATTGATGCGGTTCAA
    CAAAATAAAAAATTGCATTCTTGGTTAAAGATTTCAACATTAATTGACAATGTTAAAGTT
    AAAGATAAGTACACGGTTGAATTGAATTTGAAAGAAGCATATCAACCTGCATTGGCTGAA
    TTAGCGATGCCTCGTCCATATGTATTTGTGTCTCCAAAAGACTTTAAAAACGGTACAACA
    AAAGATGGCGTTAAAAAGTTCGATGGTACTGGTCCATTTAAATTAGGTGAACACAAAAAA
    GATGAGTCTGCAGACTTTAACAAAAATGATCAATACTGGGGCGAAAAGTCTAAACTTAAC
    AAAGTACAAGCAAAAGTAATGCCTGCTGGTGAAACAGCATTCCTATCAATGAAAAAAGGT
    GAAACGAACTTTGCCTTCACAGATGATAGAGGTACGGATAGCTTAGACAAAGACTCTTTA
    AAACAATTGAAAGATACAGGTGACTATCAAGTTAAGCGTAGTCAACCTATGAATACGAAA
    ATGTTAGTTGTCAATTCTGGTAAAAAAGATAACGCTGTCAGTGACAAAACAGTCAGACAA
    GCGATTGGTCATATGGTAAACAGAGATAAAATTGCCAAAGAAATTTTAGATGGTCAAGAA
    AAACCAGCAACTCAATTATTTGCGAAAAATGTAACAGACATTAATTTCGATATGCCAACA
    CGTAAGTATGACCTTAAAAAAGCAGAATCATTATTAGATGAAGCTGGTTGGAAGAAAGGT
    AAAGACAGCGATGTTCGTCAAAAAGATGGTAAAAACCTTGAAATGGCAATGTACTATGAC
    AAAGGTTCTTCAAGTCAGAAAGAACAAGCAGAATACTTACAAGCAGAATTTAAGAAAATG
    GGCATTAAGTTAAACATCAATGGCGAAACATCAGATAAAATTGCTGAACGTCGTACTTCT
    GGTGATTATGACTTAATGTTCAACCAAACTTGGGGATTATTGTACGATCCACAAAGTACT
    ATTGCAGCATTTAAAGCGAAAAATGGTTATGAAAGTGCAACATCAGGCATTGAGAACAAA
    GATAAAATATACAACAGCATTGATGACGCATTTAAAATCCAAAACGGTAAAGAGCGTTCA
    GGCGCTTATAAAAACATTTTGAAACAAATTGATGATGAAGGTATCTTTATCCCTATTTCA
    CACGGTAGTATGACAGTTGTTGCGCCGAAAGATTTAGAAAAAGTATCATTCACACAATCA
    CAGTATGAATTACCATTCAATGAAATGCAGTATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1599

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002442
  • symbol: CntA
  • description: staphylopine-dependent metal ABC transporter substrate-binding protein CntA
  • length: 532
  • theoretical pI: 9.40658
  • theoretical MW: 59959.7
  • GRAVY: -0.804135

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 788)
    and 1 more
    Metabolism Transport and binding proteins Unknown substrate ABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 115.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 277.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 9.21
    • Extracellular Score: 0.78
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.5209
    • Cell wall & surface Score: 0.0806
    • Extracellular Score: 0.3984
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.000447
    • TAT(Tat/SPI): 0.000088
    • LIPO(Sec/SPII): 0.999166
    • Cleavage Site: CS pos: 20-21. LTG-CG. Pr: 0.9998
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFHDGTPFDADAVKKNIDAVQQNKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAELAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGYESATSGIENKDKIYNSIDDAFKIQNGKERSGAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Laetitia Remy, Marie Carrière, Aurélie Derré-Bobillot, Cécilia Martini, Maurizio Sanguinetti, Elise Borezée-Durant
The Staphylococcus aureus Opp1 ABC transporter imports nickel and cobalt in zinc-depleted conditions and contributes to virulence.
Mol Microbiol: 2013, 87(4);730-43
[PubMed:23279021] [WorldCat.org] [DOI] (I p)

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