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PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002475
  • pan locus tag?: SAUPAN006108000
  • symbol: JSNZ_002475
  • pan gene symbol?: sasG
  • synonym:
  • product: E domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002475
  • symbol: JSNZ_002475
  • product: E domain-containing protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2472182..2477065
  • length: 4884
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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    ATGAGAGATAAGAAAGGACCGGTAAATAAAAGAGTAGATTTTCTATCAAATAAATTGAAT
    AAATATTCAATAAGAAAATTTACAGTTGGAACAGCATCTATTTTAATTGGCTCACTAATG
    TATTTGGGAACTCAACAAGAGGCAGAAGCAGCTGAAAACAATATTGAGAATCCAACTACA
    TTAAAAGATAATGTCCAATCAAAAGAAGTGAAGATTGAAGAAGTAACAAACAAAGACACT
    GCACCACAGGGTGTAGAAGCTAAATCTGAAGTAACTTCAAACAAAGACACAATCGAACAT
    GAACCATCAGTAAAAGCTGAAGATATATCAAAAAAGGAGGATACACCAAAAGAAGTAGCT
    GATGTTGCTGAAGTTCAGCCGAAATCGTCAGTCACTCATAACGCAGAGACACCTAAGGTT
    AGAAAAGCTCGTTCTGTTGATGAAGGCTCTTTTGATATTACAAGAGATTCTAAAAATGTA
    GTTGAATCTACCCCAATTACAATTCAAGGTAAAGAACATTTTGAAGGTTACGGAAGTGTT
    GATATACAAAGCAATCCAGAGGATCTAAAGGTATCTGAAGTCACTCGTTTCAATAAAAAA
    AGTATTGGAAAAAACGAATTGACAGGAGCATTACAATTAAAGAATAAAGTTTCTTTTAAA
    AATGACTTTGAGTTTAACATTAGAGTAGCTAATAATCATCAATCGGTAACAACTGGCGCT
    GATGGATGGGGGTTCTTATTTAGTAAAGGTGACGGTAACGAATATTTACAAAAGGGAGGT
    ATATTAGGTCCCAAAGGAATGGAAAATTCAGCAGGATTTAAAATTGATACAGGGTATAAT
    TTTAAAGATCCCATGGATAAAGAAGAGAAACAGGCAGGACAAGGATTTAAAGGGTATGGT
    ACGTTTGTAAAAACAGGTGCTGATGGAACTACAGCAAAGGTTGGAACAAACATACCAACA
    AGAGGTAAAGCGGATAATTCATTCCGATATGCAGATAATTCTATTGATACAACAGATGGA
    AAATTCCATGGTCAATTACTTAATAATTTAAAATTAGCGTACAATGAAAAATCGGGGATA
    ATGAGAGCCGAATATGCTGGTAAAATTTGGGAAGCTAATATATCAGATTTGGGATTGGAT
    AAATCTGAAGCATATAACTTTTTAATTACTTCTAGTCAAAGACAGGGAACATCTCAAGGT
    GTATATGCAAATGGTTGGATGAGAACAGATTTAAATAACTCAACATTTAAACTAACGCCT
    AATCTAGTTAATAAAACAGAAACAAAAACAGTAGAGATACCGTTCGAGACAAAACGTGAA
    TTTAATCCGAAGTTACAACCTGGTGAAGAGCGAGTGAAACAAGAAGGACAACCAGGAAGT
    AAGACAATCACAACACCAATCACAGTGAACCCATTAACAGGTGAAAAAGTTGGTGAGGGT
    CAACCAACAGAAGAGATCACAAAACAACCAGTAGATAAGATTGTAGAGTTTGGTGGCGAG
    AAAATACCGCAAGGTCATAAAGATATCTTCGATCCAAACTTACCAACAGATCAAACGGAA
    AAAGTACCAGGTAAACCAGGAATTAAGAATCCAGACACTGGAAAAGTGATCGAAGAGCCA
    GTGGATGATGTGATTAAACACGGACCAAAAACGGGTACACCAGAAACAAAAACAGTAGAG
    ATACCGTTTGAAACAAAACGTGAATTTAATCCGAAGTTACAACCTGGTGAAGAGCGAGTG
    AAACAAGAAGGACAACCAGGAAGTAAGACAATCACAACACCAATCACAGTGAACCCATTA
    ACGGGTGAAAAAGTTGGCGAAGGTCAACCAACAGAAGAGATCACAAAACAACCAGTAGAT
    AAGATTGTAGAATTCGGTGGCGAGAAAATACCGCAAGGTCATAAAGATATCTTTGATCCA
    AACTTACCAACAGATCAAACGGAAAAAGTACCAGGTAAACCAGGAATTAAGAATCCAGAC
    ACAGGAAAAGTGATCGAAGAGCCAGTGGATGATGTGATTAAACACGGACCAAAAACAGGC
    ACACCAGAAACAAAAACAGTAGAGATACCGTTTGAAACAAAACGTGAATTTAATCCAAAA
    TTACAACCTGGTGAAGAGCGAGTGAAACAAGAAGGACAACCAGGAAGTAAGACAATCACA
    ACACCAATCACAGTGAACCCATTAACGGGTGAAAAAGTTGGCGAGGGTCAACCAACAGAA
    GAGATCACAAAACAACCAGTAGATAAGATTGTAGAATTCGGTGGCGAGAAAATACCGCAA
    GGTCATAAAGATATCTTTGATCCAAACTTACCAACAGATCAAACGGAAAAAGTACCAGGT
    AAACCAGGAATTAAGAATCCAGACACAGGAAAAGTGATCGAAGAGCCAGTGGATGATGTG
    ATTAAACACGGACCAAAAACGGGTACACCAGAAACAAAAACAGTAGAGATACCGTTTGAA
    ACAAAACGTGAATTTAATCCAAAATTACAACCTGGTGAAGAGCGAGTGAAACAAGAAGGA
    CAACCAGGAAGTAAGACAATCACAACACCAATCACAGTGAACCCATTAACGGGTGAAAAA
    GTTGGCGAGGGTCAACCAACAGAAGAGATCACAAAACAACCAGTAGATAAGATTGTAGAA
    TTCGGTGGCGAGAAAATACCGCAAGGTCATAAAGATATCTTTGATCCAAACTTACCAACA
    GATCAAACGGAAAAAGTACCAGGTAAACCAGGAATCAAGAATCCAGACACAGGAAAAGTG
    ATCGAAGAGCCAGTGGATGATGTGATTAAACACGGACCAAAAACGGGTACACCAGAAACA
    AAAACAGTAGAGATACCGTTTGAAACAAAACGTGAGTTTAATCCAAAATTACAACCTGGT
    GAAGAGCGAGTGAAACAAGAGGGACAACCAGGAAGTAAGACAATCACAACACCAATCACA
    GTGAACCCATTAACAGGTGAAAAAGTTGGTGAGGGTCAACCAACAGAAGAGATCACAAAA
    CAACCAGTAGATAAGATTGTAGAGTTCGGTGGCGAGAAAATACCGCAAGGTCATAAAGAT
    ATCTTCGATCCAAACTTACCAACAGATCAAACGGAAAAAGTACCAGGTAAACCAGGAATT
    AAGAATCCAGACACAGGAAAAGTGATCGAAGAGCCAGTGGATGATGTGATTAAACACGGA
    CCAAAAACGGGTACACCAGAAACAAAAACAGTAGAGATACCGTTTGAAACAAAACGTGAA
    TTTAATCCAAAATTACAACCTGGTGAAGAGCGAGTGAAACAAGAAGGACAACCAGGAAGT
    AAGACAATCACAACACCAATCACAGTGAACCCATTAACGGGTGAAAAAGTTGGCGAGGGT
    CAACCAACAGAAGAGATCACAAAACAACCAGTAGATAAGATTGTAGAATTCGGTGGCGAG
    AAAATACCGCAAGGTCATAAAGATATCTTTGATCCAAACTTACCAACAGATCAAACGGAA
    AAAGTACCAGGTAAACCAGGAATCAAGAATCCAGACACAGGAAAAGTGATCGAAGAGCCA
    GTGGATGATGTGATTAAACACGGACCAAAAACGGGTACACCAGAAACAAAAACAGTAGAG
    ATACCGTTTGAAACAAAACGTGAGTTTAATCCAAAATTACAACCTGGTGAAGAGCGAGTG
    AAACAAGAGGGACAACCAGGAAGTAAGACAATCACAACACCAATCACAGTGAACCCATTA
    ACAGGTGAAAAAGTTGGTGAGGGTCAACCAACAGAAGAGATCACAAAACAACCAGTAGAT
    AAGATTGTAGAGTTCGGTGGCGAGAAAATACCGCAAGGTCATAAAGATATCTTCGATCCA
    AACTTACCAACAGATCAAACGGAAAAAGTACCAGGTAAACCAGGAATTAAGAATCCAGAC
    ACAGGAAAAGTGATCGAAGAGCCAGTGGATGATGTGATTAAACACGGACCAAAAACGGGT
    ACACCAGAAACAAAAACAGTAGAGATACCGTTTGAAACAAAACGTGAATTTAATCCAAAA
    TTACAACCTGGTGAAGAGCGAGTGAAACAAGAAGGACAACCAGGAAGTAAGACAATCACA
    ACACCAATCACAGTGAACCCATTAACGGGTGAAAAAGTTGGCGAGGGTCAACCAACAGAA
    GAGATCACAAAACAACCAGTAGATAAGATTGTAGAATTCGGTGGCGAGAAAATACCGCAA
    GGTCATAAAGATATCTTTGATCCAAACTTACCAACAGATCAAACGGAAAAAGTACCAGGT
    AAACCAGGAATCAAGAATCCAGACACAGGAAAAGTGATCGAAGAGCCAGTGGATGATGTG
    ATTAAACACGGACCAAAAACGGGTACACCAGAAACAAAAACAGTAGAGATACCGTTTGAA
    ACAAAACGTGAGTTTAATCCAAAATTACAACCTGGTGAAGAGCGAGTGAAACAAGAGGGA
    CAACCAGGAAGTAAGACAATCACAACACCAATCACAGTGAACCCATTAACAGGTGAAAAA
    GTTGGTGAGGGTCAACCAACAGAAGAGATCACAAAACAACCAGTAGATAAGATTGTAGAG
    TTTGGTGGCGAGAAACCAAAAGATCCAAAAGGACCTGAAAACCCAGAGAAGCCGAGCAGA
    CCAACTCATCCAAGTGGCCCAGTAAATCCTGACAATCCAGGATTATCGAAAGACAGAGCA
    AAACCAAATGAACCAGTTCATTCAATGGATAAAAATGATAAAGTTAAAAAATCTAAAATT
    GCTAAAAAATCAGTAGCTAATCAAGAGAAAAAACGAGCAGAATTACCAAAAACAGGTGCA
    GAAAGCACACAAAAAGGATTGATCTTTAGTAGTATAATTGGAATTGCTGGATTAATGTTA
    TTGGCACGTAGAAGAAAAAATTAA
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⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002475
  • symbol: JSNZ_002475
  • description: E domain-containing protein
  • length: 1627
  • theoretical pI: 5.45848
  • theoretical MW: 177974
  • GRAVY: -0.975292

⊟Function[edit | edit source]

  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 45.8)
    and 2 more
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 25)
    pullulanase, extracellular (TIGR02102; HMM-score: 18.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    G5 (CL0593) SasG_E; E domain (PF17041; HMM-score: 675.3)
    and 7 more
    G5; G5 domain (PF07501; HMM-score: 478.9)
    Concanavalin (CL0004) Bact_lectin; Bacterial lectin (PF18483; HMM-score: 80.6)
    no clan defined 2C_adapt; 2-cysteine adaptor domain (PF08793; HMM-score: 68.2)
    YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 45.7)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 32.8)
    DUF2680; Protein of unknown function (DUF2680) (PF10925; HMM-score: 20.3)
    H-NS_histone_C (CL0786) MvaT_DBD; Bacterial xenogeneic silencer MvaT, C-terminal domain (PF22055; HMM-score: 18.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • DeepLocPro: Cell wall & surface
    • Cytoplasmic Score: 0.0006
    • Cytoplasmic Membrane Score: 0.0056
    • Cell wall & surface Score: 0.8459
    • Extracellular Score: 0.1479
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 50 aa
    • SP(Sec/SPI): 0.831952
    • TAT(Tat/SPI): 0.075519
    • LIPO(Sec/SPII): 0.001918
    • Cleavage Site: CS pos: 50-51. AEA-AE. Pr: 0.8873
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGTQQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEHEPSVKAEDISKKEDTPKEVADVAEVQPKSSVTHNAETPKVRKARSVDEGSFDITRDSKNVVESTPITIQGKEHFEGYGSVDIQSNPEDLKVSEVTRFNKKSIGKNELTGALQLKNKVSFKNDFEFNIRVANNHQSVTTGADGWGFLFSKGDGNEYLQKGGILGPKGMENSAGFKIDTGYNFKDPMDKEEKQAGQGFKGYGTFVKTGADGTTAKVGTNIPTRGKADNSFRYADNSIDTTDGKFHGQLLNNLKLAYNEKSGIMRAEYAGKIWEANISDLGLDKSEAYNFLITSSQRQGTSQGVYANGWMRTDLNNSTFKLTPNLVNKTETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSGPVNPDNPGLSKDRAKPNEPVHSMDKNDKVKKSKIAKKSVANQEKKRAELPKTGAESTQKGLIFSSIIGIAGLMLLARRRKN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Fiona M Roche, Ruth Massey, Sharon J Peacock, Nicholas P J Day, Livia Visai, Pietro Speziale, Alex Lam, Mark Pallen, Timothy J Foster
Characterization of novel LPXTG-containing proteins of Staphylococcus aureus identified from genome sequences.
Microbiology (Reading): 2003, 149(Pt 3);643-654
[PubMed:12634333] [WorldCat.org] [DOI] (P p)

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