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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2392 [new locus tag: NWMN_RS13775 ]
  • pan locus tag?: SAUPAN006108000
  • symbol: NWMN_2392
  • pan gene symbol?: sasG
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2392 [new locus tag: NWMN_RS13775 ]
  • symbol: NWMN_2392
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2627840..2632345
  • length: 4506
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCAAATGAGAGATAAGAAAGGACCGGTAAATAAAAGAGTAGATTTTCTATCAAATAAA
    TTGAATAAATATTCAATAAGAAAATTTACAGTTGGAACAGCATCTATTTTAATTGGCTCA
    CTAATGTATTTGGGAACTCAACAAGAGGCAGAAGCAGCTGAAAACAATATTGAGAATCCA
    ACTACATTAAAAGATAATGTCCAATCAAAAGAAGTGAAGATTGAAGAAGTAACAAACAAA
    GACACTGCACCACAGGGTGTAGAAGCTAAATCTGAAGTAACTTCAAACAAAGACACAATC
    GAACATGAACCATCAGTAAAAGCTGAAGATATATCAAAAAAGGAGGATACACCAAAAGAA
    GTAGCTGATGTTGCTGAAGTTCAGCCGAAATCGTCAGTCACTCATAACGCAGAGACACCT
    AAGGTTAGAAAAGCTCGTTCTGTTGATGAAGGCTCTTTTGATATTACAAGAGATTCTAAA
    AATGTAGTTGAATCTACCCCAATTACAATTCAAGGTAAAGAACATTTTGAAGGTTACGGA
    AGTGTTGATATACAAAAAAAACCAACAGATTTAGGGGTATCAGAGGTAACCAGGTTTAAT
    GTTGGTAATGAAAGTAATGGTTTGATAGGAGCTTTACAATTAAAAAATAAAATAGATTTT
    AGTAAGGATTTCAATTTTAAAGTTAGAGTGGCAAATAACCATCAATCAAATACCACAGGT
    GCTGATGGTTGGGGGTTCTTATTTAGTAAAGGAAATGCAGAAGAATATTTAACTAATGGT
    GGAATCCTTGGGGATAAAGGTCTGGTAAATTCAGGCGGATTTAAAATTGATACTGGATAC
    ATTTATACAAGTTCCATGGACAAAACTGAAAAGCAAGCTGGACAAGGTTATAGAGGATAC
    GGAGCTTTTGTGAAAAATGACAGTTCTGGTAATTCACAAATGGTTGGAGAAAATATTGAT
    AAATCAAAAACTAATTTTTTAAACTATGCGGACAATTCAACTAATACATCAGATGGAAAG
    TTTCATGGGCAACGTTTAAATGATGTCATCTTAACTTATGTTGCTTCAACTGGTAAAATG
    AGAGCAGAATATGCTGGTAAAACTTGGGAGACTTCAATAACAGATTTAGGTTTATCTAAA
    AATCAGGCATATAATTTCTTAATTACATCTAGTCAAAGATGGGGCCTTAATCAAGGGATA
    AATGCAAATGGCTGGATGAGAACTGACTTGAAAGGTTCAGAGTTTACTTTTACACCAGAA
    GCGCCAAAAACAATAACAGAATTAGAAAAAAAAGTTGAAGAGATTCCATTCAAGAAAGAA
    CGTAAATTTAATCCGGATTTAGCACCAGGGACAGAAAAAGTAACAAGAGAAGGACAAAAA
    GGTGAGAAGACAATAACGACACCAACACTAAAAAATCCATTAACTGGAGTAATTATTAGT
    AAAGGTGAACCAAAAGAAGAGATTACAAAAGATCCGATTAATGAATTAACAGAATACGGA
    CCTGAAACAATAGCGCCAGGTCATCGAGACGAATTTGATCCGAAGTTACCAACAGGAAAG
    AAAGAGGAAGTTCCAGGTAAACCAGGAATTAAGAATCCAGAAACAGGAGACGTAGTTAGA
    CCGCCGGTCGATAGCGTAACAAAATATGGACCTGTAAAAGGAGACTCGATTGTAGAAAAA
    GAAGAGATTCCATTCGAGAAAGAACGTAAATTTAATCCGGATTTAGCACCAGGGACAGAA
    AAAGTAACAAGAGAAGGACAAAAAGGTGAGAAGACAATAACGACGCCAACACTAAAAAAT
    CCATTAACTGGAGAAATTATTAGTAAAGGTGAATCGAAAGAAGAAATCACAAAAGATCCG
    ATTAATGAATTAACAGAATACGGACCAGAAACGATAACACCAGGTCATCGAGACGAATTT
    GATCCGAAGTTACCAACAGGAGAGAAAGAGGAAGTTCCAGGTAAACCAGGAATTAAGAAT
    CCAGAAACAGGAGATGTAGTTAGACCACCGGTCGATAGCGTAACAAAATATGGACCTGTA
    AAAGGAGACTCGATTGTAGAAAAAGAAGAGATTCCATTCGAGAAAGAACGTAAATTTAAT
    CCGGATTTAGCACCAGGGACAGAAAAAGTAACAAGAGAAGGACAAAAAGGTGAGAAGACA
    ATAACGACACCAACACTAAAAAATCCATTAACTGGAGTAATTATTAGTAAAGGTGAACCA
    AAAGAAGAAATCACAAAAGATCCGATTAATGAATTAACAGAATACGGACCAGAAACGATA
    ACACCAGGTCATCGAGACGAATTTGATCCGAAGTTACCAACAGGAGAGAAAGAAGAAGTT
    CCAGGTAAACCAGGAATTAAGAATCCAGAAACAGGAGACGTAGTTAGACCACCGGTCGAT
    AGCGTAACAAAATATGGACCTGTAAAAGGAGACTCGATTGTAGAAAAAGAAGAAATTCCA
    TTCAAGAAAGAACGTAAATTTAATCCTGATTTAGCACCAGGGACAGAAAAAGTAACAAGA
    GAAGGACAAAAAGGTGAGAAGACAATAACGACGCCAACACTAAAAAATCCATTAACTGGA
    GAAATTATTAGTAAAGGTGAATCGAAAGAAGAAATCACAAAAGATCCGATTAATGAATTA
    ACAGAATACGGACCAGAAACGATAACACCAGGTCATCGAGACGAATTTGATCCGAAGTTA
    CCAACAGGAGAGAAAGAGGAAGTTCCAGGTAAACCAGGAATTAAGAATCCAGAAACAGGA
    GACGTAGTTAGACCACCGGTCGATAGCGTAACAAAATATGGACCTGTAAAAGGAGACTCG
    ATTGTAGAAAAAGAAGAAATTCCATTCAAGAAAGAACGTAAATTTAATCCTGATTTAGCA
    CCAGGGACAGAAAAAGTAACAAGAGAAGGACAAAAAGGTGAGAAGACAATAACGACGCCA
    ACACTAAAAAATCCATTAACTGGAGAAATTATTAGTAAAGGTGAATCGAAAGAAGAAATC
    ACAAAAGATCCGATTAATGAATTAACAGAATACGGACCAGAAACGATAACACCAGGTCAT
    CGAGACGAATTTGATCCGAAGTTACCAACAGGAGAGAAAGAGGAAGTTCCAGGTAAACCA
    GGAATTAAGAATCCAGAAACAGGAGATGTAGTTAGACCACCGGTCGATAGCGTAACAAAA
    TATGGACCTGTAAAAGGAGACTCGATTGTAGAAAAAGAAGAAATTCCATTCGAGAAAGAA
    CGTAAATTTAATCCTGATTTAGCACCAGGGACAGAAAAAGTAACAAGAGAAGGACAAAAA
    GGTGAGAAGACAATAACGACGCCAACACTAAAAAATCCATTAACTGGAGAAATTATTAGT
    AAAGGTGAATCGAAAGAAGAAATCACAAAAGATCCGATTAATGAATTAACAGAATACGGA
    CCAGAAACGATAACACCAGGTCATCGAGACGAATTTGATCCGAAGTTACCAACAGGAGAG
    AAAGAGGAAGTTCCAGGTAAACCAGGAATTAAGAATCCAGAAACAGGAGATGTAGTTAGA
    CCACCGGTCGATAGCGTAACAAAATATGGACCTGTAAAAGGAGACTCGATTGTAGAAAAA
    GAAGAAATTCCATTCGAGAAAGAACGTAAATTTAATCCTGATTTAGCACCAGGGACAGAA
    AAAGTAACAAGAGAAGGACAAAAAGGTGAGAAGACAATAACGACGCCAACACTAAAAAAT
    CCATTAACTGGAGAAATTATTAGTAAAGGTGAATCGAAAGAAGAAATCACAAAAGATCCA
    GTTAATGAATTAACAGAATTCGGTGGCGAGAAAATACCGCAAGGTCATAAAGATATCTTT
    GATCCAAACTTACCAACAGATCAAACGGAAAAAGTACCAGGTAAACCAGGAATCAAGAAT
    CCAGACACAGGAAAAGTGATCGAAGAGCCAGTGGATGATGTGATTAAACACGGACCAAAA
    ACGGGTACACCAGAAACAAAAACAGTAGAGATACCGTTTGAAACAAAACGTGAGTTTAAT
    CCAAAATTACAACCTGGTGAAGAGCGAGTGAAACAAGAAGGACAACCAGGAAGTAAGACA
    ATCACAACACCAATCACAGTGAACCCATTAACAGGTGAAAAAGTTGGCGAGGGTCAACCA
    ACAGAAGAGATCACAAAACAACCAGTAGATAAGATTGTAGAGTTCGGTGGAGAGAAACCA
    AAAGATCCAAAAGGACCTGAAAACCCAGAGAAGCCGAGCAGACCAACTCATCCAAGTGGC
    CCAGTAAATCCTAACAATCCAGGATTATCGAAAGACAGAGCAAAACCAAATGGCCCAGTT
    CATTCAATGGATAAAAATGATAAAGTTAAAAAATCTAAAATTGCTAAAGAATCAGTAGCT
    AATCAAGAGAAAAAACGAGCAGAATTACCAAAAACAGGTTTAGAAAGCACGCAAAAAGGT
    TTGATCTTTAGTAGTATAATTGGAATTGCTGGATTAATGTTATTGGCTCGTAGAAGAAAG
    AATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3840
    3900
    3960
    4020
    4080
    4140
    4200
    4260
    4320
    4380
    4440
    4500
    4506

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2392 [new locus tag: NWMN_RS13775 ]
  • symbol: NWMN_2392
  • description: hypothetical protein
  • length: 1501
  • theoretical pI: 5.21479
  • theoretical MW: 164723
  • GRAVY: -0.978414

⊟Function[edit | edit source]

  • TIGRFAM:
    calcium-translocating P-type ATPase, PMCA-type (TIGR01517; EC 3.6.3.8; HMM-score: 57.8)
    and 3 more
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 46.1)
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 23.6)
    zinc metalloproteinase 18-residue repeat (TIGR04227; HMM-score: 9)
  • TheSEED: data available for NCTC8325
  • PFAM:
    G5 (CL0593) SasG_E; E domain (PF17041; HMM-score: 640.5)
    and 6 more
    G5; G5 domain (PF07501; HMM-score: 316.2)
    no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 44.2)
    DMP12; DNA-mimic protein (PF16779; HMM-score: 31.8)
    NTF2 (CL0051) DUF3828; Protein of unknown function (DUF3828) (PF12883; HMM-score: 25)
    no clan defined Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 24.9)
    2C_adapt; 2-cysteine adaptor domain (PF08793; HMM-score: 19.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 3
    • Predicted Cleavage Site: found LPxTG motif :LPKTG
  • SignalP: Signal peptide SP(Sec/SPI) length 52 aa
    • SP(Sec/SPI): 0.795355
    • TAT(Tat/SPI): 0.076073
    • LIPO(Sec/SPII): 0.001563
    • Cleavage Site: CS pos: 52-53. AEA-AE. Pr: 0.8532
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQMRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGTQQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEHEPSVKAEDISKKEDTPKEVADVAEVQPKSSVTHNAETPKVRKARSVDEGSFDITRDSKNVVESTPITIQGKEHFEGYGSVDIQKKPTDLGVSEVTRFNVGNESNGLIGALQLKNKIDFSKDFNFKVRVANNHQSNTTGADGWGFLFSKGNAEEYLTNGGILGDKGLVNSGGFKIDTGYIYTSSMDKTEKQAGQGYRGYGAFVKNDSSGNSQMVGENIDKSKTNFLNYADNSTNTSDGKFHGQRLNDVILTYVASTGKMRAEYAGKTWETSITDLGLSKNQAYNFLITSSQRWGLNQGINANGWMRTDLKGSEFTFTPEAPKTITELEKKVEEIPFKKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGVIISKGEPKEEITKDPINELTEYGPETIAPGHRDEFDPKLPTGKKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFEKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFEKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGVIISKGEPKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFKKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFKKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFEKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFEKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPVNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSGPVNPNNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQKGLIFSSIIGIAGLMLLARRRKN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Fiona M Roche, Ruth Massey, Sharon J Peacock, Nicholas P J Day, Livia Visai, Pietro Speziale, Alex Lam, Mark Pallen, Timothy J Foster
Characterization of novel LPXTG-containing proteins of Staphylococcus aureus identified from genome sequences.
Microbiology (Reading): 2003, 149(Pt 3);643-654
[PubMed:12634333] [WorldCat.org] [DOI] (P p)

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