PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2436
• pan locus tag^?: SAUPAN006111000
• symbol: SAUSA300_2436
• pan gene symbol^?: sasG
• synonym:
• product: putative cell wall surface anchor family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2436
• symbol: SAUSA300_2436
• product: putative cell wall surface anchor family protein
• replicon: chromosome
• strand: -
• coordinates: 2624916..2626250
• length: 1335
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914248 NCBI
• RefSeq: YP_495070 NCBI
• BioCyc:
• MicrobesOnline: 1293951 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  481
  541
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  661
  721
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  1021
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  1141
  1201
  1261
  1321
  
  ATGAGAGATAAGAAAGGACCGGTAAATAAAAGAGTAGATTTTCTATCAAATAAATTGAAT
  AAATATTCAATAAGAAAATTTACAGTTGGAACAGCATCTATTTTAATTGGCTCACTAATG
  TATTTGGGAACTCAACAAGAGGCAGAAGCAGCTGAAAACAATATTGAGAATCCAACTACA
  TTAAAAGATAATGTCCAATCAAAAGAAGTGAAGATTGAAGAAGTAACAAACAAAGACACT
  GCACCACAGGGTGTAGAAGCTAAATCTGAAGTAACTTCAAACAAAGACACAATCGAACAT
  GAACCATCAGTAAAAGCTGAAGATATATCAAAAAAGGAGGATACACCAAAAGAAGTAGCT
  GATGTTGCTGAAGTTCAGCCGAAATCGTCAGTCACTCATAACGCAGAGACACCTAAGGTT
  AGAAAAGCTCGTTCTGTTGATGAAGGCTCTTTTGATATTACAAGAGATTCTAAAAATGTA
  GTTGAATCTACCCCAATTACAATTCAAGGTAAAGAACATTTTGAAGGTTACGGAAGTGTT
  GATATACAAAAAAAACCAACAGATTTAGGGGTATCAGAGGTAACCAGGTTTAATGTTGGT
  AATGAAAGTAATGGTTTGATAGGAGCTTTACAATTAAAAAATAAAATAGATTTTAGTAAG
  GATTTCAATTTTAAAGTTAGAGTGGCAAATAACCATCAATCAAATACCACAGGTGCTGAT
  GGTTGGGGGTTCTTATTTAGTAAAGGAAATGCAGAAGAATATTTAACTAATGGTGGAATC
  CTTGGGGATAAAGGTCTGGTAAATTCAGGCGGATTTAAAATTGATACTGGATACATTTAT
  ACAAGTTCCATGGACAAAACTGAAAAGCAAGCTGGACAAGGTTATAGAGGATACGGAGCT
  TTTGTGAAAAATGACAGTTCTGGTAATTCACAAATGGTTGGAGAAAATATTGATAAATCA
  AAAACTAATTTTTTAAACTATGCGGACAATTCAACTAATACATCAGATGGAAAGTTTCAT
  GGGCAACGTTTAAATGATGTCATCTTAACTTATGTTGCTTCAACTGGTAAAATGAGAGCA
  GAATATGCTGGTAAAACTTGGGAGACTTCAATAACAGATTTAGGTTTATCTAAAAATCAG
  GCATATAATTTCTTAATTACATCTAGTCAAAGATGGGGCCTTAATCAAGGGATAAATGCA
  AATGGCTGGATGAGAACTGACTTGAAAGGTTCAGAGTTTACTTTTACACCAGAAGCGCCA
  AAAACAATAACAGAATTAGAAAAAAAGTTGAAGAGATTCCATTCAAGAAAGAACGTAAAT
  TTAATCCGGATTTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_2436
• symbol: SAUSA300_2436
• description: putative cell wall surface anchor family protein
• length: 444
• theoretical pI: 9.15585
• theoretical MW: 49024.2
• GRAVY: -0.750676

⊟Function

• TIGRFAM:
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 48.1)
• TheSEED  :
  • Virulence-associated cell-wall-anchored protein SasG (LPXTG motif), binding to squamous nasal epithelial cells
  Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Virulence-associated cell-wall-anchored protein SasG (LPXTG motif), binding to squamous nasal epithelial cells
• PFAM:
  Concanavalin (CL0004) Bact_lectin; Bacterial lectin (PF18483; HMM-score: 95.9)
  and 1 more
  no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 47.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 9.2
  • Extracellular Score: 0.78
  • Internal Helices: 0
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.0002
  • Cell wall & surface Score: 0.02
  • Extracellular Score: 0.9798
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.5
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 9
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide SP(Sec/SPI) length 50 aa
  • SP(Sec/SPI): 0.831952
  • TAT(Tat/SPI): 0.075519
  • LIPO(Sec/SPII): 0.001918
  • Cleavage Site: CS pos: 50-51. AEA-AE. Pr: 0.8873
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162069 NCBI
• RefSeq: YP_495070 NCBI
• UniProt: A0A0H2XJV9 UniProt

⊟Protein sequence

• MRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGTQQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEHEPSVKAEDISKKEDTPKEVADVAEVQPKSSVTHNAETPKVRKARSVDEGSFDITRDSKNVVESTPITIQGKEHFEGYGSVDIQKKPTDLGVSEVTRFNVGNESNGLIGALQLKNKIDFSKDFNFKVRVANNHQSNTTGADGWGFLFSKGNAEEYLTNGGILGDKGLVNSGGFKIDTGYIYTSSMDKTEKQAGQGYRGYGAFVKNDSSGNSQMVGENIDKSKTNFLNYADNSTNTSDGKFHGQRLNDVILTYVASTGKMRAEYAGKTWETSITDLGLSKNQAYNFLITSSQRWGLNQGINANGWMRTDLKGSEFTFTPEAPKTITELEKKLKRFHSRKNVNLIRI

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications