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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2505 [new locus tag: SACOL_RS13130 ]
  • pan locus tag?: SAUPAN006107000
  • symbol: SACOL2505
  • pan gene symbol?: sasG
  • synonym:
  • product: cell wall surface anchor family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2505 [new locus tag: SACOL_RS13130 ]
  • symbol: SACOL2505
  • product: cell wall surface anchor family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2559220..2562951
  • length: 3732
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGAGATAAGAAAGGACCGGTAAATAAAAGAGTAGATTTTCTATCAAATAAATTGAAT
    AAATATTCAATAAGAAAATTTACAGTTGGAACAGCATCTATTTTAATTGGCTCACTAATG
    TATTTGGGAACTCAACAAGAGGCAGAAGCAGCTGAAAACAATATTGAGAATCCAACTACA
    TTAAAAGATAATGTCCAATCAAAAGAAGTGAAGATTGAAGAAGTAACAAACAAAGACACT
    GCACCACAGGGTGTAGAAGCTAAATCTGAAGTAACTTCAAACAAAGACACAATCGAACAT
    GAACCATCAGTAAAAGCTGAAGATATATCAAAAAAGGAGGATACACCAAAAGAAGTAGCT
    GATGTTGCTGAAGTTCAGCCGAAATCGTCAGTCACTCATAACGCAGAGACACCTAAGGTT
    AGAAAAGCTCGTTCTGTTGATGAAGGCTCTTTTGATATTACAAGAGATTCTAAAAATGTA
    GTTGAATCTACCCCAATTACAATTCAAGGTAAAGAACATTTTGAAGGTTACGGAAGTGTT
    GATATACAAAAAAAACCAACAGATTTAGGGGTATCAGAGGTAACCAGGTTTAATGTTGGT
    AATGAAAGTAATGGTTTGATAGGAGCTTTACAATTAAAAAATAAAATAGATTTTAGTAAG
    GATTTCAATTTTAAAGTTAGAGTGGCAAATAACCATCAATCAAATACCACAGGTGCTGAT
    GGTTGGGGGTTCTTATTTAGTAAAGGAAATGCAGAAGAATATTTAACTAATGGTGGAATC
    CTTGGGGATAAAGGTCTGGTAAATTCAGGCGGATTTAAAATTGATACTGGATACATTTAT
    ACAAGTTCCATGGACAAAACTGAAAAGCAAGCTGGACAAGGTTATAGAGGATACGGAGCT
    TTTGTGAAAAATGACAGTTCTGGTAATTCACAAATGGTTGGAGAAAATATTGATAAATCA
    AAAACTAATTTTTTAAACTATGCGGACAATTCAACTAATACATCAGATGGAAAGTTTCAT
    GGGCAACGTTTAAATGATGTCATCTTAACTTATGTTGCTTCAACTGGTAAAATGAGAGCA
    GAATATGCTGGTAAAACTTGGGAGACTTCAATAACAGATTTAGGTTTATCTAAAAATCAG
    GCATATAATTTCTTAATTACATCTAGTCAAAGATGGGGCCTTAATCAAGGGATAAATGCA
    AATGGCTGGATGAGAACTGACTTGAAAGGTTCAGAGTTTACTTTTACACCAGAAGCGCCA
    AAAACAATAACAGAATTAGAAAAAAAAGTTGAAGAGATTCCATTCAAGAAAGAACGTAAA
    TTTAATCCGGATTTAGCACCAGGGACAGAAAAAGTAACAAGAGAAGGACAAAAAGGTGAG
    AAGACAATAACGACACCAACACTAAAAAATCCATTAACTGGAGTAATTATTAGTAAAGGT
    GAACCAAAAGAAGAGATTACAAAAGATCCGATTAATGAATTAACAGAATACGGACCTGAA
    ACAATAGCGCCAGGTCATCGAGACGAATTTGATCCGAAGTTACCAACAGGAGAGAAAGAG
    GAAGTTCCAGGTAAACCAGGAATTAAGAATCCAGAAACAGGAGACGTAGTTAGACCACCG
    GTCGATAGCGTAACAAAATATGGACCTGTAAAAGGAGACTCGATTGTAGAAAAAGAAGAG
    ATTCCATTCGAGAAAGAACGTAAATTTAATCCGGATTTAGCACCAGGGACAGAAAAAGTA
    ACAAGAGAAGGACAAAAAGGTGAGAAGACAATAACGACACCAACACTAAAAAATCCATTA
    ACTGGAGTAATTATTAGTAAAGGTGAACCAAAAGAAGAAATCACAAAAGATCCGATTAAT
    GAATTAACAGAATACGGACCAGAAACGATAACACCAGGTCATCGAGACGAATTTGATCCG
    AAGTTACCAACAGGAGAGAAAGAGGAAGTTCCAGGTAAACCAGGAATTAAGAATCCAGAA
    ACAGGAGACGTAGTTAGACCACCGGTCGATAGCGTAACAAAATATGGACCTGTAAAAGGA
    GACTCGATTGTAGAAAAAGAAGAAATTCCATTCAAGAAAGAACGTAAATTTAATCCGGAT
    TTAGCACCAGGGACAGAAAAAGTAACAAGAGAAGGACAAAAAGGTGAGAAGACAATAACG
    ACGCCAACACTAAAAAATCCATTAACTGGAGAAATTATTAGTAAAGGTGAATCGAAAGAA
    GAAATCACAAAAGATCCGATTAATGAATTAACAGAATACGGACCAGAAACGATAACACCA
    GGTCATCGAGACGAATTTGATCCGAAGTTACCAACAGGAGAGAAAGAGGAAGTTCCAGGT
    AAACCAGGAATTAAGAATCCAGAAACAGGAGACGTAGTTAGACCACCGGTCGATAGCGTA
    ACAAAATATGGACCTGTAAAAGGAGACTCGATTGTAGAAAAAGAAGAAATTCCATTCAAG
    AAAGAACGTAAATTTAATCCTGATTTAGCACCAGGGACAGAAAAAGTAACAAGAGAAGGA
    CAAAAAGGTGAGAAGACAATAACGACGCCAACACTAAAAAATCCATTAACTGGAGAAATT
    ATTAGTAAAGGTGAATCGAAAGAAGAAATCACAAAAGATCCGATTAATGAATTAACAGAA
    TACGGACCAGAAACGATAACACCAGGTCATCGAGACGAATTTGATCCGAAGTTACCAACA
    GGAGAGAAAGAGGAAGTTCCAGGTAAACCAGGAATTAAGAATCCAGAAACAGGAGATGTA
    GTTAGACCACCGGTCGATAGCGTAACAAAATATGGACCTGTAAAAGGAGACTCGATTGTA
    GAAAAAGAAGAAATTCCATTCGAGAAAGAACGTAAATTTAATCCTGATTTAGCACCAGGG
    ACAGAAAAAGTAACAAGAGAAGGACAAAAAGGTGAGAAGACAATAACGACGCCAACACTA
    AAAAATCCATTAACTGGAGAAATTATTAGTAAAGGTGAATCGAAAGAAGAAATCACAAAA
    GATCCAGTTAATGAATTAACAGAATTCGGTGGCGAGAAAATACCGCAAGGTCATAAAGAT
    ATCTTTGATCCAAACTTACCAACAGATCAAACGGAAAAAGTACCAGGTAAACCAGGAATC
    AAGAATCCAGACACAGGAAAAGTGATCGAAGAGCCAGTGGATGATGTGATTAAACACGGA
    CCAAAAACGGGTACACCAGAAACAAAAACAGTAGAGATACCGTTTGAAACAAAACGTGAG
    TTTAATCCAAAATTACAACCTGGTGAAGAGCGAGTGAAACAAGAAGGACAACCAGGAAGT
    AAGACAATCACAACACCAATCACAGTGAACCCATTAACAGGTGAAAAAGTTGGCGAGGGT
    CAACCAACAGAAGAGATCACAAAACAACCAGTAGATAAGATTGTAGAGTTCGGTGGAGAG
    AAACCAAAAGATCCAAAAGGACCTGAAAACCCAGAGAAGCCGAGCAGACCAACTCATCCA
    AGTGGCCCAGTAAATCCTAACAATCCAGGATTATCGAAAGACAGAGCAAAACCAAATGGC
    CCAGTTCATTCAATGGATAAAAATGATAAAGTTAAAAAATCTAAAATTGCTAAAGAATCA
    GTAGCTAATCAAGAGAAAAAACGAGCAGAATTACCAAAAACAGGTTTAGAAAGCACGCAA
    AAAGGTTTGATCTTTAGTAGTATAATTGGAATTGCTGGATTAATGTTATTGGCTCGTAGA
    AGAAAGAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3732

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2505 [new locus tag: SACOL_RS13130 ]
  • symbol: SACOL2505
  • description: cell wall surface anchor family protein
  • length: 1243
  • theoretical pI: 5.49702
  • theoretical MW: 136344
  • GRAVY: -0.956718

⊟Function[edit | edit source]

  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 46.4)
    calcium-translocating P-type ATPase, PMCA-type (TIGR01517; EC 3.6.3.8; HMM-score: 39.2)
    and 2 more
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 23.9)
    zinc metalloproteinase 18-residue repeat (TIGR04227; HMM-score: 10)
  • TheSEED  :
    • Virulence-associated cell-wall-anchored protein SasG (LPXTG motif), binding to squamous nasal epithelial cells
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Virulence-associated cell-wall-anchored protein SasG (LPXTG motif), binding to squamous nasal epithelial cells
  • PFAM:
    G5 (CL0593) SasG_E; E domain (PF17041; HMM-score: 461.6)
    and 8 more
    G5; G5 domain (PF07501; HMM-score: 241.3)
    no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 44.4)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 25.2)
    DMP12; DNA-mimic protein (PF16779; HMM-score: 22.5)
    Ribosomal_L28; Ribosomal L28 family (PF00830; HMM-score: 22.1)
    2C_adapt; 2-cysteine adaptor domain (PF08793; HMM-score: 18.5)
    NTF2 (CL0051) DUF3828; Protein of unknown function (DUF3828) (PF12883; HMM-score: 16.3)
    HAD (CL0137) Cation_ATPase; Cation transport ATPase (P-type) (PF13246; HMM-score: 8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.5
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 3
    • Predicted Cleavage Site: found LPxTG motif :LPKTG
  • SignalP: Signal peptide SP(Sec/SPI) length 50 aa
    • SP(Sec/SPI): 0.831952
    • TAT(Tat/SPI): 0.075519
    • LIPO(Sec/SPII): 0.001918
    • Cleavage Site: CS pos: 50-51. AEA-AE. Pr: 0.8873
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGTQQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEHEPSVKAEDISKKEDTPKEVADVAEVQPKSSVTHNAETPKVRKARSVDEGSFDITRDSKNVVESTPITIQGKEHFEGYGSVDIQKKPTDLGVSEVTRFNVGNESNGLIGALQLKNKIDFSKDFNFKVRVANNHQSNTTGADGWGFLFSKGNAEEYLTNGGILGDKGLVNSGGFKIDTGYIYTSSMDKTEKQAGQGYRGYGAFVKNDSSGNSQMVGENIDKSKTNFLNYADNSTNTSDGKFHGQRLNDVILTYVASTGKMRAEYAGKTWETSITDLGLSKNQAYNFLITSSQRWGLNQGINANGWMRTDLKGSEFTFTPEAPKTITELEKKVEEIPFKKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGVIISKGEPKEEITKDPINELTEYGPETIAPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFEKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGVIISKGEPKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFKKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFKKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFEKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPVNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSGPVNPNNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQKGLIFSSIIGIAGLMLLARRRKN

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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