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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0778 [new locus tag: NWMN_RS04405 ]
  • pan locus tag?: SAUPAN002835000
  • symbol: NWMN_0778
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0778 [new locus tag: NWMN_RS04405 ]
  • symbol: NWMN_0778
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 868976..869371
  • length: 396
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    GTGATGATGATGGCTATTGTAAATAAAGTGATAATTGTTGAAGGAAAATCTGATAAAAAA
    AGGGTGCAACAGGTTATTGCAGAACCAGTCAATATTATTTGTACTCATGGAACAATGAGT
    ATAGATAAGCTTGATGATATGATAGAATCACTGTATGATAAACAAGTTTTTGTATTAGCC
    GATTCTGATGACGAAGGAGATCGAATTAGAAATTGGTTTAAACGTTATTTGAGTGAAAGT
    GAACATATATTTATTGATAAAACTTACTGTCAAGTTGCGAATTGCCCCAAACAATATTTG
    GCGCATGTACTTTCAAAACATGGCTTTACTTGTAAGAAAGAAACACCTCTTTTACCGAAT
    ATAAATAATGAAAGGTTAGTTTTAGTAAATGAATAA
    60
    120
    180
    240
    300
    360
    396

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0778 [new locus tag: NWMN_RS04405 ]
  • symbol: NWMN_0778
  • description: hypothetical protein
  • length: 131
  • theoretical pI: 6.505
  • theoretical MW: 15145.5
  • GRAVY: -0.254198

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Transcription RNA processing ribonuclease M5 (TIGR00334; EC 3.1.26.8; HMM-score: 51.4)
    and 1 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA gyrase, B subunit (TIGR01059; EC 5.99.1.3; HMM-score: 15.1)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Toprim-like (CL0413) Toprim; Toprim domain (PF01751; HMM-score: 21.4)
    and 4 more
    no clan defined DUF2100; Uncharacterized protein conserved in archaea (DUF2100) (PF09873; HMM-score: 16.5)
    Toprim-like (CL0413) Toprim_4; Toprim domain (PF13662; HMM-score: 13.4)
    Toprim_2; Toprim-like (PF13155; HMM-score: 13.3)
    no clan defined Calc_CGRP_IAPP; Calcitonin / CGRP / IAPP family (PF00214; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.78
    • Cytoplasmic Membrane Score: 8.16
    • Cellwall Score: 0.06
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006879
    • TAT(Tat/SPI): 0.000282
    • LIPO(Sec/SPII): 0.00147
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMMMAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDMIESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLSKHGFTCKKETPLLPNINNERLVLVNE

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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