From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0848 [new locus tag: NWMN_RS04775 ]
  • pan locus tag?: SAUPAN003119000
  • symbol: NWMN_0848
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0848 [new locus tag: NWMN_RS04775 ]
  • symbol: NWMN_0848
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 944196..944837
  • length: 642
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGAAATTTTAAATGATTCTGTAAAAAGTTTTGAAAAACTTTATTTACAATTACAAGAT
    GGTTTTCCAGTGATTGTTCCTACTGATACTAATTATAATTTATGTAGTTTACCTAACAAC
    GATCTTTGTATCGATAAAATATTTGAATATAAAAAGCGATCGAAAGATAAGCCATTATCA
    TTATTTATTGATAAGCCAGAGGATTGGAAATTGTATGGAGATAATCAAAATACGGAAATA
    GTTGATAAACTAGTTGAAATATTTTGGCCTGGACCATTAAATATTATTTTAAAAAATAAA
    ACAAGCTATAATTATATGCTCAATAATTCAGATAGTATAGCTATAGGATGTGTACAAAAT
    AAAACGATGAGAAGATTTATTTCATATATTAATTCACCTATAGCAATTACTTCAGCAAAT
    ATATCTGGAACTGCCGATGATATTTTAATAACTGAAAATGAAGCAATTAAGCACATGGGC
    GAAAATGTAAGATATATGCTAAGAAGTCAAAATAAAACTAACTATAAAACATCTAGTACG
    ATTATTAAAGTGACAGATAATAAAATTGAATTATTAAGAGAAGGAGATATAAAGTTTGAA
    GAAATAAAAGAAAGACTAGGTACAGGTATTATTTATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    642

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0848 [new locus tag: NWMN_RS04775 ]
  • symbol: NWMN_0848
  • description: hypothetical protein
  • length: 213
  • theoretical pI: 5.15196
  • theoretical MW: 24548.9
  • GRAVY: -0.41831

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein, Sua5/YciO/YrdC/YwlC family (TIGR00057; HMM-score: 115.7)
    and 1 more
    Genetic information processing Protein fate Protein modification and repair carbamoyltransferase HypF (TIGR00143; HMM-score: 15.6)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, Pigments Tetrapyrroles Experimental tye  Sua5 YciO YrdC YwlC family protein
    and 6 more
    RNA Metabolism RNA processing and modification t6A synthesis in bacteria  TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
    tRNA modification Archaea  TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
    tRNA mods Archaea  TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
    YrdC-YciO  TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
    YrdC-YciO-Sua5 and associated protein families  TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
    LMPTP YwlE cluster  TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
  • PFAM:
    no clan defined Sua5_yciO_yrdC; Telomere recombination (PF01300; HMM-score: 136.2)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00926
    • TAT(Tat/SPI): 0.00024
    • LIPO(Sec/SPII): 0.001025
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCIDKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLNIILKNKTSYNYMLNNSDSIAIGCVQNKTMRRFISYINSPIAITSANISGTADDILITENEAIKHMGENVRYMLRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEIKERLGTGIIYE

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]