NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL0982 [new locus tag: SACOL_RS05025 ]
• pan locus tag^?: SAUPAN003119000
• symbol: SACOL0982
• pan gene symbol^?: —
• synonym:
• product: Sua5/YciO/YrdC/YwlC family protein

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL0982 [new locus tag: SACOL_RS05025 ]
• symbol: SACOL0982
• product: Sua5/YciO/YrdC/YwlC family protein
• replicon: chromosome
• strand: +
• coordinates: 991023..991664
• length: 642
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3237875 NCBI
• RefSeq: YP_185850 NCBI
• BioCyc:
• MicrobesOnline: 912451 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGAAATTTTAAATGATTCTGTAAAAAGTTTTGAAAAACTTTATTTACAATTACAAGAT
  GGTTTTCCAGTGATTGTTCCTACTGATACTAATTATAATTTATGTAGTTTACCTAACAAC
  GATCTTTGTATCGATAAAATATTTGAATATAAAAAGCGATCGAAAGATAAGCCATTATCA
  TTATTTATTGATAAGCCAGAGGATTGGAAATTGTATGGAGATAATCAAAATACGGAAATA
  GTTGATAAACTAGTTGAAATATTTTGGCCTGGACCATTAAATATTATTTTAAAAAATAAA
  ACAAGCTATAATTATATGCTCAATAATTCAGATAGTATAGCTATAGGATGTGTACAAAAT
  AAAACGATGAGAAGATTTATTTCATATATTAATTCACCTATAGCAATTACTTCAGCAAAT
  ATATCTGGAACTGCCGATGATATTTTAATAACTGAAAATGAAGCAATTAAGCACATGGGC
  GAAAATGTAAGATATATGCTAAGAAGTCAAAATAAAACTAACTATAAAACATCTAGTACG
  ATTATTAAAGTGACAGATAATAAAATTGAATTATTAAGAGAAGGAGATATAAAGTTTGAA
  GAAATAAAAGAAAGACTAGGTACAGGTATTATTTATGAATAA
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⊟Protein

⊟General

• locus tag: SACOL0982 [new locus tag: SACOL_RS05025 ]
• symbol: SACOL0982
• description: Sua5/YciO/YrdC/YwlC family protein
• length: 213
• theoretical pI: 5.15196
• theoretical MW: 24548.9
• GRAVY: -0.41831

⊟Function

• TIGRFAM:
  Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA threonylcarbamoyl adenosine modification protein, Sua5/YciO/YrdC/YwlC family (TIGR00057; HMM-score: 115.7)
  and 1 more
  Genetic information processingProtein fateProtein modification and repaircarbamoyltransferase HypF (TIGR00143; HMM-score: 15.6)
• TheSEED:  
  Cofactors, Vitamins, Prosthetic Groups, PigmentsTetrapyrrolesExperimental tye Sua5 YciO YrdC YwlC family protein
  and 6 more
  RNA MetabolismRNA processing and modificationt6A synthesis in bacteria TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
  tRNA modification Archaea TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
  tRNA mods Archaea TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
  YrdC-YciO TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
  YrdC-YciO-Sua5 and associated protein families TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
  LMPTP YwlE cluster TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
• PFAM:
  no clan definedSua5_yciO_yrdC; Telomere recombination (PF01300; HMM-score: 136.2)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00926
  • TAT(Tat/SPI): 0.00024
  • LIPO(Sec/SPII): 0.001025
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 57651669 NCBI
• RefSeq: YP_185850 NCBI
• UniProt: A0A0H2WZF6 UniProt

⊟Protein sequence

• MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCIDKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLNIILKNKTSYNYMLNNSDSIAIGCVQNKTMRRFISYINSPIAITSANISGTADDILITENEAIKHMGENVRYMLRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEIKERLGTGIIYE

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SACOL0981 > SACOL0982 > SACOL0983 > SACOL0984

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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