From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0883 [new locus tag: NWMN_RS04945 ]
  • pan locus tag?: SAUPAN003187000
  • symbol: NWMN_0883
  • pan gene symbol?: —
  • synonym:
  • product: Na+/alanine symporter family protein

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0883 [new locus tag: NWMN_RS04945 ]
  • symbol: NWMN_0883
  • product: Na+/alanine symporter family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 979147..980775
  • length: 1629
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    TTGAAGTGTCTCGTGCTTAATCGTATAATATTTTTATGTTTAAACAATAGGGGGAATCTT
    ATGATTGAAAAATTAGTAACCTTTTTAAATGAGGTTGTTTGGAGTAAGCCATTAGTTTAT
    GGTTTGCTAATTACTGGTGTGCTATTTACATTGCGTATGCGATTTTTTCAAGTTAGACAT
    TTTAAAGAAATGATTCGATTAATGTTTCAAGGAGAGAAGTCTCCTAATGGTATTTCAAGT
    TTTCAAGCGATAGCCATGTCTTTAGCAGGCAGGGTTGGTACAGGTAATATTGTCGGTGTA
    TCTACTGCAATATTTATAGGAGGACCTGGTGCAGTATTTTGGATGTGGATTACTGCGTTT
    TTAGGTGCAAGTAGTGCTTTTATTGAATCTACACTTGGTCAAATATTCAAGAGAGTTGAA
    AATAATGAATACCGTGGTGGACCAGCGTATTATATTGAATATGGTATTGGTGGTAAATTT
    GGTAAAATTTACGGAATTATCTTTGCTATTGTTACGATTATCTCAGTAGGTCTATTGCTT
    CCTGGTGTGCAATCTAACGCTATAGCAAGTTCTATGCATAATGCGATTCATGTTCCACAA
    TGGTTAATGGGTGGTATTGTTGTAGTTATTTTGGGATTAATTATTTTTGGTGGTGTACGT
    AGTATTGCCAATGTTGCAACAGCCGTTGTACCATTTATGGCAATTATTTACATACTGATG
    GCTGTCATTATCATTTGTATCAATATACAAGAAGTGCCAGCGTTATTTGCATTAATTTTC
    AAATCAGCATTTGGATTACAATCTGCTTTTGGTGGTATCGTTGGCGCAATGATAGAGATT
    GGTGTTAAACGTGGATTATATTCAAATGAGGCTGGTCAAGGTACAGGTCCACACGCAGCA
    GCGGCAGCAGAAGTATCACATCCAAGTAAACAAGGTCTAGTACAAGCATTTTCAGTTTAT
    ATTGATACATTATTTGTATGTACTGCAACTGCTCTGATTATACTTATTTCTGGTACATAT
    AATGTGACTGATGGTACGGTTAATGCGAATGGCACACCGCATTTAATTAAAGATGGCGGT
    ATTTATGTTGAAAATGCAACAGGTAAAGATTATTCAGGTACTGCGATGTATGCACAAGCC
    GGCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTA
    GGTTCGTACTTTATTGCATTTGCTTTATTCTTCTTTGCATTTACTACAATTTTGTCGTAC
    TACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCAAAATAATCAAGTTTCA
    TCGATATTTATTAATATTGCTCGTGTGATTATTTTGTTCGCTACATTTTACGGTGCAGTT
    AAAACAGCTGATGTAGCATGGGCATTCGGTGATTTAGGTGTAGGTCTAATGGCTTGGTTA
    AATATCATTGCGATTTGGATTTTACATAAGCCTGCCGTAAATGCTTTAAAAGATTATGAA
    ATTCAAAAGAAACGTTTAGGCAACGGTTATAATGCAGTTTATCAACCTGATCCGAATAAA
    TTACCTAATGCTGTCTTTTGGTTGAAGACGTATCCAGAACGTTTAAAACAAGCACGTGCC
    AAAAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1629


⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0883 [new locus tag: NWMN_RS04945 ]
  • symbol: NWMN_0883
  • description: Na+/alanine symporter family protein
  • length: 542
  • theoretical pI: 9.74281
  • theoretical MW: 58961.7
  • GRAVY: 0.53524

⊟Function[edit | edit source]

  • TIGRFAM:
    amino acid carrier protein (TIGR00835; HMM-score: 465.2)
  • TheSEED  :
    • sodium/alanine symporter family protein
  • PFAM:
    APC (CL0062) Na_Ala_symp; Sodium:alanine symporter family (PF01235; HMM-score: 501.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9998
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0002
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002162
    • TAT(Tat/SPI): 0.000174
    • LIPO(Sec/SPII): 0.001608
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKCLVLNRIIFLCLNNRGNLMIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEMIRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLGASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLLPGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYILMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTGPHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPHLIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFFAFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFGDLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWLKTYPERLKQARAKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]


⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=NWMN_0883&oldid=120213"