PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0966 [new locus tag: NWMN_RS05415 ]
• pan locus tag^?: SAUPAN003324000
• symbol: potB
• pan gene symbol^?: potB
• synonym:
• product: spermidine/putrescine ABC transporter permease protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0966 [new locus tag: NWMN_RS05415 ]
• symbol: potB
• product: spermidine/putrescine ABC transporter permease protein
• replicon: chromosome
• strand: +
• coordinates: 1072229..1073026
• length: 798
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332282 NCBI
• RefSeq: YP_001332000 NCBI
• BioCyc:
• MicrobesOnline: 3706517 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGCGTAATACTAATAAATTTCTCTTAATCCCGTATTTACTATGGATGGTTATATTTATT
  ATTGTACCAGTTGTATTACTCATTTATTTTTCATTTTTAGATATCAATGGACATTTTAGT
  TTCACGAATTATCAACAAATTTTTACTACAAAATATTTGAAAATGTTTGCATATTCAATT
  TTATATGCCGCTTCAATAACAATTATTACCTTGACTATCAGTTATCCAGCTGCCTATTAT
  ATTACTCGTTCGAAATTTCAAAATATCTTATTAATGATAATGATTATTCCAACATGGATA
  AATTTATTGTTAAAGACATATGCTTTTATAGGTTTATTAAGTCATGATGGCGTGATTAAT
  CAATTTTTCCACTTATTTAATTTACCATCATTCAATTTGTTATTTACAACTGGTGCGTTT
  TTAGTAGTGGCAAGTTACATTTATATACCATTTATGATTTTACCTATATTTAATAGCATG
  AAAGCAATTCCTAATAATTTATTGCAGGCCTCAAGTGATTTAGGTGCCAGTCCTTTCTAT
  ACTTTTAGAAAAGTAATCATGCCGTTAACAAAAGAAGGTGTTATGACTGGGATTCAAGTA
  ACCTTTATTCCATCACTTTCATTATTTATGATTACAAGATTAATTGCAGGTAATAAAGTC
  ATAAATATAGGTACGGCAATAGAGGAACAATTTTTAACAATTCAAAATTATGGTATGGGA
  TCAACTATAGCTATATTCTTAATTGTATTTATGGCATTCATTTTAATCATTACAAAATCA
  TCTAATGGGAGAGGGTGA
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⊟Protein

⊟General

• locus tag: NWMN_0966 [new locus tag: NWMN_RS05415 ]
• symbol: PotB
• description: spermidine/putrescine ABC transporter permease protein
• length: 265
• theoretical pI: 10.0419
• theoretical MW: 30150
• GRAVY: 0.869434

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, permease protein (TIGR03262; HMM-score: 77)
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 64.6)
  NifC-like ABC-type porter (TIGR01581; HMM-score: 64.1)
  and 9 more
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein (TIGR00969; HMM-score: 54)
  2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 49.6)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 39.3)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysW (TIGR02140; HMM-score: 31.1)
  2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 17.5)
  choline ABC transporter, permease protein (TIGR03416; HMM-score: 15)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 14.2)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 13)
  Metabolism Transport and binding proteins Other thiamine/thiamine pyrophosphate ABC transporter, permease protein (TIGR01253; HMM-score: 9.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 40.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005295
  • TAT(Tat/SPI): 0.000153
  • LIPO(Sec/SPII): 0.008606
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 151221178 NCBI
• RefSeq: YP_001332000 NCBI
• UniProt: A0A0H3K832 UniProt

⊟Protein sequence

• MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNYQQIFTTKYLKMFAYSILYAASITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINLLLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSMKAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGNKVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0964 > potA > potB > potC > NWMN_0968 > NWMN_0969

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications