PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0951 [new locus tag: SA_RS05385 ]
• pan locus tag^?: SAUPAN003324000
• symbol: potB
• pan gene symbol^?: potB
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0951 [new locus tag: SA_RS05385 ]
• symbol: potB
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1077954..1078751
• length: 798
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123775 NCBI
• RefSeq: NP_374218 NCBI
• BioCyc: see SA_RS05385
• MicrobesOnline: 103244 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGCGTAATACTAATAAATTTCTCTTAATCCCGTATTTACTATGGATGGTTATATTTATT
  ATTGTACCAGTTGTATTACTCATTTATTTTTCATTTTTAGATATCAATGGACATTTTAGT
  TTCACGAATTATCAACAAATTTTTACTACAAAATATTTGAAAATGTTTGCATATTCAATT
  TTATATGCCGCTTTAATAACAATTATTACGTTGGCTATCAGTTATCCAGCTGCCTATTAT
  ATTACTCGTTCGAAATTTCAAAATATCTTATTAATGATAATGATTATTCCAACATGGATA
  AATTTATTGTTAAAGACATATGCTTTTATAGGTTTATTAAGTCATGATGGCGTGATTAAT
  CAATTTTTCCACTTATTTAATTTACCATCATTCAATTTGTTATTTACAACTGGTGCGTTT
  TTAGTAGTGGCAAGTTACATTTATATACCATTTATGATTTTACCTATATTTAATAGCATG
  AAAGCAATTCCTAATAATTTATTGCAGGCCTCAAGTGATTTAGGTGCCAGTCCTTTCTAT
  ACTTTTAGAAAAGTAATCATGCCGTTAACAAAAGAAGGTGTTATGACTGGGATTCAAGTA
  ACCTTTATTCCATCACTTTCATTATTTATGATTACAAGATTAATTGCAGGTAATAAAGTC
  ATAAATATAGGTACGGCAATAGAGGAACAATTTTTAACAATTCAAAATTATGGTATGGGA
  TCAACTATAGCTATATTCTTAATTGTATTTATGGCATTCATTTTAATCATTACAAAATCA
  TCTAATGGGAGAGGGTGA
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⊟Protein

⊟General

• locus tag: SA0951 [new locus tag: SA_RS05385 ]
• symbol: PotB
• description: hypothetical protein
• length: 265
• theoretical pI: 10.0419
• theoretical MW: 30146.1
• GRAVY: 0.896226

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, permease protein (TIGR03262; HMM-score: 77.1)
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 65)
  NifC-like ABC-type porter (TIGR01581; HMM-score: 64.4)
  and 11 more
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein (TIGR00969; HMM-score: 54.3)
  2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 48.8)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 39.5)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysW (TIGR02140; HMM-score: 33.3)
  2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 17.3)
  choline ABC transporter, permease protein (TIGR03416; HMM-score: 15.5)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 14.4)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 14.3)
  Metabolism Transport and binding proteins Other thiamine/thiamine pyrophosphate ABC transporter, permease protein (TIGR01253; HMM-score: 11.8)
  Cellular processes Cellular processes Pathogenesis type III secretion protein, YscU/HrpY family (TIGR01404; HMM-score: 7.9)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type III secretion protein, YscU/HrpY family (TIGR01404; HMM-score: 7.9)
• TheSEED  :
  • Spermidine/putrescine import ABC transporter, permease subunit PotB
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Polyamine Metabolism  Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1)
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 40.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003778
  • TAT(Tat/SPI): 0.000146
  • LIPO(Sec/SPII): 0.0136
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 15926685 NCBI
• RefSeq: NP_374218 NCBI
• UniProt: A0A0H3JPM1 UniProt

⊟Protein sequence

• MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNYQQIFTTKYLKMFAYSILYAALITIITLAISYPAAYYITRSKFQNILLMIMIIPTWINLLLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSMKAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGNKVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0949 > potA > potB > potC > potD

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications