PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1119 [new locus tag: NWMN_RS06320 ]
• pan locus tag^?: SAUPAN003490000
• symbol: NWMN_1119
• pan gene symbol^?: rqcH
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1119 [new locus tag: NWMN_RS06320 ]
• symbol: NWMN_1119
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1224836..1226533
• length: 1698
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330684 NCBI
• RefSeq: YP_001332153 NCBI
• BioCyc:
• MicrobesOnline: 3706671 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGGCTTATGATGGCTTATTTACAAAGAAAATGGTTGAGTCTCTACAATTTTTAACAACA
  GGACGCGTTCACAAAATCAATCAACCTGATAATGACACGATACTAATGGTTGTACGTCAA
  AATAGACAAAACCATCAATTGTTATTGTCAATCCATCCAAACTTTTCAAGATTACAATTG
  ACTACTAAAAAATATGATAATCCATTTAATCCACCCATGTTTGCGCGTGTTTTTAGAAAA
  CACTTAGAAGGTGGTATTATCGAATCGATTAAGCAAATTGGTAATGATCGTCGCATTGAA
  ATCGATATAAAGAGTAAAGATGAAATTGGCGATACTATTTACCGCACTGTCATCCTTGAG
  ATTATGGGTAAACATAGTAACTTAATTTTAGTAGATGAAAATCGCAAAATAATTGAAGGA
  TTTAAACACTTAACACCAAATACGAATCACTATCGTACAGTAATGCCAGGATTTAATTAT
  GAAGCACCACCTACTCAGCACAAAATAAATCCGTATGATATTACAGGTGCAGAGGTGTTG
  AAATATATCGATTTTAACGCAGGTAATATTGCTAAACAATTATTGAATCAGTTTGAAGGA
  TTTAGCCCTTTAATTACGAATGAAATCGTTAGTCGTCGTCAATTTATGACTTCATCAACA
  TTACCAGAAGCATTTGACGAAGTAATGGCAGAAACCAAGTTACCACCTACTCCTATTTTT
  CATAAAAATCATGAAACAGGTAAAGAGGATTTCTATTTTATAAAGTTAAATCAATTTAAT
  GATGATACAGTTACATACGATTCATTAAATGATTTGCTTGATCGTTTTTATGATGCGCGT
  GGCGAACGTGAACGCGTTAAACAACGTGCGAATGATTTAGTTCGATTTGTTCAACAGCAG
  TTGCACAAATATCAAAATAAATTAGCGAAGTTGATTGAAGAATATGAGCAGTCTAAAAAT
  AAAGATACTGAACAGTTATATGGTGAATTGATCACTGCTAATATATATCGAATTAAGCAA
  GGCGATAAAGAAGTGACGGCATTGAATTATTATACGAATGAAGAAGTTGTCATTCCTTTA
  AATCCTACAAAATCCCCATCAGCAAATGCTCAATATTATTATAAACAATATAATCGTATG
  AAAACGAGAGAACGTGAATTACAACATCAAATTCAATTGACGAAAGACAATATAGATTAT
  TTTTCAACAATCGAACAACAATTACATCATATTTCTGTCCATGACATTGATGAAATTAGA
  GATGAATTAGCAGAACAAGGCTTTATGAAACAGCGTAAAAATCAAACTAAGAAAAAGAAA
  GCGCAGATTCAATTACAACATTATGTATCAACTGATGGCGACGATATATATGTTGGTAAG
  AATAACAAGCAAAATGATTATTTAACAAATAAAAAAGCTAAAAAAACTCACACATGGTTA
  CACACAAAAGATATTCCTGGTTCACATGTCGTTATATTTAATGATGCACCAAGTGATACG
  ACAATCAAGGAAGCGGCTATGTTAGCAGGATACTTTTCAAAAGCTGGTAATTCTGGACAA
  ATACCTGTTGATTATACATTAATTAAAAATGTGCATAAACCATCAGGTGCAAAGCCTGGG
  TTTGTAACATATGACAATCAAAAAACTTTGTATGCTACACCTGATTATGAACTTATTCAA
  AAAATGAAACAATCATAA
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⊟Protein

⊟General

• locus tag: NWMN_1119 [new locus tag: NWMN_RS06320 ]
• symbol: NWMN_1119
• description: hypothetical protein
• length: 565
• theoretical pI: 9.17033
• theoretical MW: 65784
• GRAVY: -0.772212

⊟Function

• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  H2TH (CL0303) FbpA; Fibronectin-binding protein A N-terminus (FbpA) (PF05833; HMM-score: 473.1)
  and 5 more
  no clan defined DUF814; Domain of unknown function (DUF814) (PF05670; HMM-score: 91.8)
  Apolipoprotein; Apolipoprotein A1/A4/E domain (PF01442; HMM-score: 16)
  Tropomyosin-lke (CL0452) CLZ; C-terminal leucine zipper domain of cyclic nucleotide-gated channels (PF16526; HMM-score: 15.3)
  no clan defined GAT; GAT domain (PF03127; HMM-score: 14.2)
  HotDog (CL0050) YiiD_C; Putative thioesterase (yiiD_Cterm) (PF09500; HMM-score: 11.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002293
  • TAT(Tat/SPI): 0.000215
  • LIPO(Sec/SPII): 0.001228
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221331 NCBI
• RefSeq: YP_001332153 NCBI
• UniProt: A0A0H3K7Q0 UniProt

⊟Protein sequence

• MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQNHQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEIDIKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNYEAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFMTSSTLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRFYDARGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITANIYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQLTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVSTDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAMLAGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications