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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1101 [new locus tag: SAUSA300_RS05965 ]
  • pan locus tag?: SAUPAN003490000
  • symbol: SAUSA300_1101
  • pan gene symbol?: rqcH
  • synonym:
  • product: putative fibronectin/fibrinogen binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1101 [new locus tag: SAUSA300_RS05965 ]
  • symbol: SAUSA300_1101
  • product: putative fibronectin/fibrinogen binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1204825..1206522
  • length: 1698
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCTTATGATGGCTTATTTACAAAGAAAATGGTTGAGTCTCTACAATTTTTAACAACA
    GGACGCGTTCACAAAATCAATCAACCTGATAATGACACGATACTAATGGTTGTACGTCAA
    AATAGACAAAACCATCAATTGTTATTGTCAATCCATCCAAACTTTTCAAGATTACAATTG
    ACTACTAAAAAATATGATAATCCATTTAATCCACCCATGTTTGCGCGTGTTTTTAGAAAA
    CACTTAGAAGGTGGTATTATCGAATCGATTAAGCAAATTGGTAATGATCGTCGCATTGAA
    ATCGATATAAAGAGTAAAGATGAAATTGGCGATACTATTTACCGCACTGTCATCCTTGAG
    ATTATGGGTAAACATAGTAACTTAATTTTAGTAGATGAAAATCGCAAAATAATTGAAGGA
    TTTAAACACTTAACACCAAATACGAATCACTATCGTACAGTAATGCCAGGATTTAATTAT
    GAAGCACCACCTACTCAGCACAAAATAAATCCGTATGATATTACAGGTGCAGAGGTGTTG
    AAATATATCGATTTTAACGCAGGTAATATTGCTAAACAATTATTGAATCAGTTTGAAGGA
    TTTAGCCCTTTAATTACGAATGAAATCGTTAGTCGTCGTCAATTTATTACTTCATCAACA
    TTACCAGAAGCATTTGACGAAGTAATGGCAGAAACCAAGTTACCACCTACTCCTATTTTT
    CATAAAAATCATGAAACAGGTAAAGAGGATTTCTATTTTATAAAGTTAAATCAATTTAAT
    GATGATACAGTTACATACGATTCATTAAATGATTTGCTTGATCGTTTTTATGATGCGCGT
    GGCGAACGTGAACGCGTTAAACAACGTGCGAATGATTTAGTTCGATTTGTTCAACAGCAG
    TTGTACAAATATCAAAATAAATTAGCGAAGTTGATTGAAGAATATGAGCAGTCTAAAAAT
    AAAGATACTGAACAGTTATATGGTGAATTGATCACTGCTAATATATATCGAATTAAGCAA
    GGCGATAAAGAAGTGACGGCATTGAATTATTATACGAATGAAGAAGTTGTCATTCCTTTA
    AATCCTACAAAATCCCCATCAGCAAATGCTCAATATTATTATAAACAATATAATCGTATG
    AAAACGAGAGAACGTGAATTACAACATCAAATTCAATTGACGAAAGACAATATAGATTAT
    TTTTCAACAATCGAACAACAATTACATCATATTTCTGTCCATGACATTGATGAAATTAGA
    GATGAATTAGCAGAACAAGGCTTTATGAAACAGCGTAAAAATCAAACTAAGAAAAAGAAA
    GCGCAGATTCAATTACAACATTATGTATCAACTGATGGCGACGATATATATGTTGGTAAG
    AATAACAAGCAAAATGATTATTTAACAAATAAAAAAGCTAAAAAAACTCACACATGGTTA
    CACACAAAAGATATTCCTGGTTCACATGTCGTTATATTTAATGATGCACCAAGTGATACG
    ACAATCAAGGAAGCGGCTATGTTAGCAGGATACTTTTCAAAAGCTGGTAATTCTGGACAA
    ATACCTGTTGATTATACATTAATTAAAAATGTGCATAAACCATCAGGTGCAAAGCCTGGG
    TTTGTAACATATGACAATCAAAAAACTTTGTATGCTACACCTGATTATGAACTTATTCAA
    AAAATGAAACAATCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1698

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1101 [new locus tag: SAUSA300_RS05965 ]
  • symbol: SAUSA300_1101
  • description: putative fibronectin/fibrinogen binding protein
  • length: 565
  • theoretical pI: 9.15917
  • theoretical MW: 65792
  • GRAVY: -0.764248

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Fibronectin/fibrinogen-binding protein
  • PFAM:
    H2TH (CL0303) FbpA; Fibronectin-binding protein A N-terminus (FbpA) (PF05833; HMM-score: 470.6)
    and 5 more
    no clan defined DUF814; Domain of unknown function (DUF814) (PF05670; HMM-score: 91.7)
    Tropomyosin-lke (CL0452) CLZ; C-terminal leucine zipper domain of cyclic nucleotide-gated channels (PF16526; HMM-score: 14.2)
    no clan defined Apolipoprotein; Apolipoprotein A1/A4/E domain (PF01442; HMM-score: 13.1)
    HotDog (CL0050) YiiD_C; Putative thioesterase (yiiD_Cterm) (PF09500; HMM-score: 11)
    no clan defined GAT; GAT domain (PF03127; HMM-score: 10.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002293
    • TAT(Tat/SPI): 0.000215
    • LIPO(Sec/SPII): 0.001228
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQNHQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEIDIKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNYEAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFITSSTLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRFYDARGERERVKQRANDLVRFVQQQLYKYQNKLAKLIEEYEQSKNKDTEQLYGELITANIYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQLTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVSTDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAMLAGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

Ribosome-associated quality control protein H (RqcH) detects 50S ribosomes that are obstructed by malformed nascent proteins. It adds a poly-alanine tail to the malformed protein's C-terminus to flag it for ClpXP degradation.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Iryna Lytvynenko, Helge Paternoga, Anna Thrun, Annika Balke, Tina A Müller, Christina H Chiang, Katja Nagler, George Tsaprailis, Simon Anders, Ilka Bischofs, Julie A Maupin-Furlow, Christian M T Spahn, Claudio A P Joazeiro
    Alanine Tails Signal Proteolysis in Bacterial Ribosome-Associated Quality Control.
    Cell: 2019, 178(1);76-90.e22
    [PubMed:31155236] [WorldCat.org] [DOI] (I p)
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