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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1410 [new locus tag: NWMN_RS07960 ]
  • pan locus tag?: SAUPAN004036000
  • symbol: NWMN_1410
  • pan gene symbol?: proC
  • synonym:
  • product: pyrroline-5-carboxylate reductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1410 [new locus tag: NWMN_RS07960 ]
  • symbol: NWMN_1410
  • product: pyrroline-5-carboxylate reductase
  • replicon: chromosome
  • strand: +
  • coordinates: 1578193..1579008
  • length: 816
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAACTCGTATTTTATGGAGCTGGTAATATGGCACAAGCTATATTTACAGGAATTATT
    AACTCAAGCAACTTAGATGCCAATGATATATATTTAACAAATAAATCTAATGAACAAGCT
    TTAAAAGCATTCGCTGAAAAACTAGGTGTTAACTATAGTTATGATGATGCGACATTATTA
    AAAGATGCAGATTATGTATTTTTAGGTACCAAACCACATGATTTTGATGCTCTAGCAACA
    CGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTATGGCAGGTATTCCG
    ATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGAATTATGCCAAAC
    ACAAATGCGCAAGTTGGACACTCTGTTACTGGCATTAGTTTTTCAAACAACTTTGACCCT
    AAATCTAAAGATGAAATTAACGATTTAGTTAAAGCATTTGGTTCTGTAATTGAAGTATCA
    GAAGATCATTTACATCAAGTAACAGCTATCACCGGAAGCGGCCCAGCATTTTTATATCAT
    GTATTCGAGCAATATGTTAAAGCTGGTACGAAACTTGGTCTAGAAAAAGAACAAGTTGAA
    GAATCTATACCCAACCTTATTATAGGTACAAGTAAGATGATTGAAACGTCAGAATTGAGC
    ATGGCTCAATTAAAAAAAAAAATACCTCTAAAGGGGGTACAAAACAAGCCTGGCCTTGAT
    ACATTGTCACAATATGATTTAGTATCTATTTTCGAAGATTGTCTAAACGCTGCCGTCGAC
    CGTAGTATTGAACTTTCTAATATAGAAGACCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    816

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1410 [new locus tag: NWMN_RS07960 ]
  • symbol: NWMN_1410
  • description: pyrroline-5-carboxylate reductase
  • length: 271
  • theoretical pI: 4.77284
  • theoretical MW: 29855.7
  • GRAVY: -0.180074

⊟Function[edit | edit source]

  • reaction:
    EC 1.5.1.2?  ExPASy
    Pyrroline-5-carboxylate reductase L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 256.1)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    6PGD_C (CL0106) P5CR_dimer; Pyrroline-5-carboxylate reductase dimerisation (PF14748; HMM-score: 68.2)
    NADP_Rossmann (CL0063) F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 60.3)
    and 5 more
    GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 16.9)
    PDH; Prephenate dehydrogenase (PF02153; HMM-score: 16.7)
    NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 14.5)
    no clan defined DUF4918; Domain of unknown function (DUF4918) (PF16265; HMM-score: 13.5)
    NADP_Rossmann (CL0063) ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.238193
    • TAT(Tat/SPI): 0.001855
    • LIPO(Sec/SPII): 0.021152
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAFAEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPIDYIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVSEDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIPNLIIGTSKMIETSELSMAQLKKKIPLKGVQNKPGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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