PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1334 [new locus tag: SA_RS07560 ]
• pan locus tag^?: SAUPAN004036000
• symbol: SA1334
• pan gene symbol^?: proC
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1334 [new locus tag: SA_RS07560 ]
• symbol: SA1334
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1540500..1541315
• length: 816
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124174 NCBI
• RefSeq: NP_374617 NCBI
• BioCyc: see SA_RS07560
• MicrobesOnline: 103643 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAACTCGTATTTTATGGAGCTGGTAATATGGCACAAGCTATATTTACAGGAATTATT
  AACTCAAGCAACTTAGATGCCAATGATATATATTTAACAAATAAATCTAATGAACAAGCT
  TTAAAAGCATTCGCTGAAAAACTAGGTGTTAACTATAGTTATGATGATGCGACATTATTA
  AAAGATGCAGATTATGTTTTTTTAGGTACCAAACCGCATGACTTTGATGCTCTAGCAACA
  CGCATCAAACCACATATCACAAAAGACAATTGCTTCATTTCAATTATGGCAGGTATTCCG
  ATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGAATTATGCCAAAC
  ACAAATGCGCAAGTTGGACACTCAGTTACTGGCATTAGTTTTTCAAACAACTTTGACCCT
  AAATCTAAAGATGAAATTAACGATTTAGTTAAAGCATTTGGTTCTGTAATTGAAGTATCA
  GAGGATCATTTACATCAAGTAACAGCTATCACCGGAAGCGGCCCAGCATTTTTATATCAT
  GTATTCGAGCAATACGTTAAAGCTGGTACGAAACTTGGTCTAGAAAAAGAACAAGTTGAA
  GAATCTATACGCAACCTTATTATAGGTACAAGTAAGATGATTGAACGTTCAGATTTGAGC
  ATGGCTCAATTAAGAAAAAATATTACCTCTAAAGGTGGTACGACACAAGCTGGCCTTGAT
  ACATTGTCACAATATGATTTAGTATCTATTTTCGAAGATTGTCTAAATGCTGCCGTCGAC
  CGTAGTATTGAACTTTCTAATGTAGAAGACCAATAA
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⊟Protein

⊟General

• locus tag: SA1334 [new locus tag: SA_RS07560 ]
• symbol: SA1334
• description: hypothetical protein
• length: 271
• theoretical pI: 4.76728
• theoretical MW: 29825.5
• GRAVY: -0.202583

⊟Function

• reaction:
  EC 1.5.1.2?  ExPASy
  Pyrroline-5-carboxylate reductase L-proline + NAD(P)⁺ = 1-pyrroline-5-carboxylate + NAD(P)H
• TIGRFAM:
  Metabolism Amino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 286.4)
• TheSEED  :
  • Pyrroline-5-carboxylate reductase (EC 1.5.1.2)
  Amino Acids and Derivatives Proline and 4-hydroxyproline A Hypothetical Protein Related to Proline Metabolism  Pyrroline-5-carboxylate reductase (EC 1.5.1.2)
  and 2 more
  Amino Acids and Derivatives Proline and 4-hydroxyproline Proline Synthesis  Pyrroline-5-carboxylate reductase (EC 1.5.1.2)
  Amino Acids and Derivatives Proline and 4-hydroxyproline Proline, 4-hydroxyproline uptake and utilization  Pyrroline-5-carboxylate reductase (EC 1.5.1.2)
• PFAM:
  6PGD_C (CL0106) P5CR_dimer; Pyrroline-5-carboxylate reductase dimerisation (PF14748; HMM-score: 94.8)
  and 6 more
  NADP_Rossmann (CL0063) F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 60.8)
  GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 16.9)
  PDH; Prephenate dehydrogenase (PF02153; HMM-score: 16.8)
  no clan defined DUF4918; Domain of unknown function (DUF4918) (PF16265; HMM-score: 14.9)
  NADP_Rossmann (CL0063) NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 14.5)
  ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.238193
  • TAT(Tat/SPI): 0.001855
  • LIPO(Sec/SPII): 0.021152
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927084 NCBI
• RefSeq: NP_374617 NCBI
• UniProt: Q7A5G8 UniProt

⊟Protein sequence

• MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAFAEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPIDYIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVSEDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSDLSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNVEDQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)