PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1452 [new locus tag: SAUSA300_RS07930 ]
• pan locus tag^?: SAUPAN004036000
• symbol: proC
• pan gene symbol^?: proC
• synonym:
• product: pyrroline-5-carboxylate reductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1452 [new locus tag: SAUSA300_RS07930 ]
• symbol: proC
• product: pyrroline-5-carboxylate reductase
• replicon: chromosome
• strand: +
• coordinates: 1604865..1605650
• length: 786
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914537 NCBI
• RefSeq: YP_494148 NCBI
• BioCyc: see SAUSA300_RS07930
• MicrobesOnline: 1292967 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGCACAAGCTATATTTACAGGAATTATTAACTCAAGCAACTTAGATGCCAATGATATA
  TATTTAACAAATAAATCTAATGAACAAGCTTTAAAAGCATTCGCTGAAAAACTAGGTGTT
  AACTATAGTTATGATGATGCGACATTATTAAAAGATGCAGATTATGTATTTTTAGGTACC
  AAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAAT
  TGCTTCATTTCAATTATGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGC
  CAAAATCCAGTTGCTAGAATTATGCCAAACACAAATGCGCAAGTTGGACACTCTGTTACT
  GGCATTAGTTTTTCAAACAACTTTGACCCTAAATCTAAAGATGAAATTAACGATTTAGTT
  AAAGCATTTGGTTCTGTAATTGAAGTATCAGAAGATCATTTACATCAAGTAACAGCTATC
  ACCGGAAGCGGCCCAGCATTTTTATATCATGTATTCGAGCAATATGTTAAAGCTGGTACG
  AAACTTGGTCTAGAAAAAGAACAAGTTGAAGAATCTATACGCAACCTTATTATAGGTACA
  AGTAAGATGATTGAACGTTCAGATTTGAGCATGGCTCAATTAAGAAAAAATATTACCTCT
  AAAGGTGGTACGACACAAGCTGGCCTTGATACATTGTCACAATATGATTTAGTATCTATT
  TTCGAAGATTGTCTAAACGCTGCCGTCGACCGTAGTATTGAACTTTCTAATATAGAAGAC
  CAATAA
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  786
  

⊟Protein

⊟General

• locus tag: SAUSA300_1452 [new locus tag: SAUSA300_RS07930 ]
• symbol: ProC
• description: pyrroline-5-carboxylate reductase
• length: 261
• theoretical pI: 4.67633
• theoretical MW: 28758.2
• GRAVY: -0.228352

⊟Function

• reaction:
  EC 1.5.1.2?  ExPASy
  Pyrroline-5-carboxylate reductase L-proline + NAD(P)⁺ = 1-pyrroline-5-carboxylate + NAD(P)H
• TIGRFAM:
  Metabolism Amino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 272.2)
• TheSEED  :
  • Pyrroline-5-carboxylate reductase (EC 1.5.1.2)
  Amino Acids and Derivatives Proline and 4-hydroxyproline A Hypothetical Protein Related to Proline Metabolism  Pyrroline-5-carboxylate reductase (EC 1.5.1.2)
  and 2 more
  Amino Acids and Derivatives Proline and 4-hydroxyproline Proline Synthesis  Pyrroline-5-carboxylate reductase (EC 1.5.1.2)
  Amino Acids and Derivatives Proline and 4-hydroxyproline Proline, 4-hydroxyproline uptake and utilization  Pyrroline-5-carboxylate reductase (EC 1.5.1.2)
• PFAM:
  6PGD_C (CL0106) P5CR_dimer; Pyrroline-5-carboxylate reductase dimerisation (PF14748; HMM-score: 94.8)
  and 5 more
  NADP_Rossmann (CL0063) F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 47.7)
  PDH; Prephenate dehydrogenase (PF02153; HMM-score: 16.9)
  no clan defined DUF4918; Domain of unknown function (DUF4918) (PF16265; HMM-score: 15)
  NADP_Rossmann (CL0063) NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 14.6)
  ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009616
  • TAT(Tat/SPI): 0.000268
  • LIPO(Sec/SPII): 0.000925
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160301 NCBI
• RefSeq: YP_494148 NCBI
• UniProt: A0A0H2XGB3 UniProt

⊟Protein sequence

• MAQAIFTGIINSSNLDANDIYLTNKSNEQALKAFAEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPIDYIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVSEDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSDLSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications