PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1551 [new locus tag: NWMN_RS08695 ]
• pan locus tag^?: SAUPAN004255000
• symbol: mreC
• pan gene symbol^?: mreC
• synonym:
• product: rod shape-determining protein MreC

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1551 [new locus tag: NWMN_RS08695 ]
• symbol: mreC
• product: rod shape-determining protein MreC
• replicon: chromosome
• strand: -
• coordinates: 1716997..1717839
• length: 843
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332165 NCBI
• RefSeq: YP_001332585 NCBI
• BioCyc:
• MicrobesOnline: 3707103 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  
  GTGCTTAAGTTTTTTAAAAATAACAAATTAATTGTTGTTTTATGTGCAATTATCGTTTTT
  ATTGCATTAATTGGGCTGTCCATACGTTCACAATCTCAATCACCTCCTGAACAATATATA
  GGTGATTCTGTGTCTTTTGGACAACGAGTTGTGAGTTATCCAGTTAATTTTGTTGCTGGT
  ACGATTGGGGACTTTTTTAAAAAAGGAGATTCTAAAGAATCTAAAAATAAGATTAGCCAG
  TTAGAATCTAAGAACCAACAATTAGAAGCGGAAAATGAAAAATTAAAAAAAGAGCTTGAT
  TTAAAAGATATTTCAAAATTTGATCCTATTTCTACTACGGTTTTGGCAAGAAATCCGGAT
  CAGTGGATGAATACAATTGTAATTGATAAGGGATCTAAATCAGGTATAACTTCAAATATG
  GCTGTGATGACATCACAAGGTTTTGTTGGAAGAGTTACTAAAGTTAATAAATTTTCTTCA
  CAAGTTGATTTAATCTCAACTAATACACGTGCGGGTAAATTATCTGTAAATATACAACAC
  GGTTCTAAAAATATATTTGGTTTAATTGATCGTTATGATGAAAAGAACTCAGAACTTGTA
  ATTAGTGACATTAATAATAGAGATAATATCTCAAAAGGTGATAAAGTCGTTACAAGTGGA
  TTAGCTGATCAACTACCAAGTAATTTATATATAGGAGAAGTGACTAAGGTTCAAAATGAT
  CAATACGGCTTAGCTAAAGAAGTTAGGGTTAAGACTGGTGCGGACTTAACAGATTTGAGT
  CATGTTTATGTTGCAAAAAGAGATCCTAAAACAATTCCTGATGATGAAAGCAGGGATAAA
  TAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  843
  

⊟Protein

⊟General

• locus tag: NWMN_1551 [new locus tag: NWMN_RS08695 ]
• symbol: MreC
• description: rod shape-determining protein MreC
• length: 280
• theoretical pI: 9.59859
• theoretical MW: 31010
• GRAVY: -0.398929

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan rod shape-determining protein MreC (TIGR00219; HMM-score: 147)
  and 4 more
  Genetic information processing Mobile and extrachromosomal element functions Plasmid functions integrating conjugative element protein, PFL_4705 family (TIGR03752; HMM-score: 18.1)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking preprotein translocase, YajC subunit (TIGR00739; HMM-score: 15)
  integrating conjugative element protein, PFL_4669 family (TIGR03761; HMM-score: 12.1)
  Cellular processes Cellular processes Cell division cell division protein FtsL (TIGR02209; HMM-score: 4.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined MreC; rod shape-determining protein MreC (PF04085; HMM-score: 135.4)
  and 12 more
  YajC; Preprotein translocase subunit (PF02699; HMM-score: 22.5)
  TSC22; TSC-22/dip/bun family (PF01166; HMM-score: 18.7)
  FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 17.4)
  no clan defined YabB; Initiation control protein YabA (PF06156; HMM-score: 16.6)
  IncA; IncA protein (PF04156; HMM-score: 15.4)
  OppC_N; N-terminal TM domain of oligopeptide transport permease C (PF12911; HMM-score: 15.2)
  DUF1845; Domain of unknown function (DUF1845) (PF08900; HMM-score: 15.1)
  bZIP (CL0018) bZIP_1; bZIP transcription factor (PF00170; HMM-score: 15)
  no clan defined Kinetocho_Slk19; Central kinetochore-associated (PF12709; HMM-score: 14.7)
  bZIP (CL0018) bZIP_2; Basic region leucine zipper (PF07716; HMM-score: 14.5)
  no clan defined Swi5; Swi5 (PF07061; HMM-score: 12.8)
  Shugoshin_N; Shugoshin N-terminal coiled-coil region (PF07558; HMM-score: 7.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide SP(Sec/SPI) length 32 aa
  • SP(Sec/SPI): 0.795076
  • TAT(Tat/SPI): 0.001816
  • LIPO(Sec/SPII): 0.137729
  • Cleavage Site: CS pos: 32-33. SQS-QS. Pr: 0.5493
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 151221763 NCBI
• RefSeq: YP_001332585 NCBI
• UniProt: A0A0H3KD55 UniProt

⊟Protein sequence

• MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSVSFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLKDISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSSQVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVTSGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDESRDK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: mreD < mreC

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications