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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01759
  • pan locus tag?: SAUPAN004255000
  • symbol: SAOUHSC_01759
  • pan gene symbol?: mreC
  • synonym:
  • product: rod shape-determining protein MreC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01759
  • symbol: SAOUHSC_01759
  • product: rod shape-determining protein MreC
  • replicon: chromosome
  • strand: -
  • coordinates: 1660198..1661040
  • length: 843
  • essential: no [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGCTTAAGTTTTTTAAAAATAACAAATTAATTGTTGTTTTATGTGCAATTATCGTTTTT
    ATTGCATTAATTGGGCTGTCCATACGTTCACAATCTCAATCACCTCCTGAACAATATATA
    GGTGATTCTGTGTCTTTTGGACAACGAGTTGTGAGTTATCCAGTTAATTTTGTTGCTGGT
    ACGATTGGGGACTTTTTTAAAAAAGGAGATTCTAAAGAATCTAAAAATAAGATTAGCCAG
    TTAGAATCTAAGAACCAACAATTAGAAGCGGAAAATGAAAAATTAAAAAAAGAGCTTGAT
    TTAAAAGATATTTCAAAATTTGATCCTATTTCTACTACGGTTTTGGCAAGAAATCCGGAT
    CAGTGGATGAATACAATTGTAATTGATAAGGGATCTAAATCAGGTATAACTTCAAATATG
    GCTGTGATGACATCACAAGGTTTTGTTGGAAGAGTTACTAAAGTTAATAAATTTTCTTCA
    CAAGTTGATTTAATCTCAACTAATACACGTGCGGGTAAATTATCTGTAAATATACAACAC
    GGTTCTAAAAATATATTTGGTTTAATTGATCGTTATGATGAAAAGAACTCAGAACTTGTA
    ATTAGTGACATTAATAATAGAGATAATATCTCAAAAGGTGATAAAGTCGTTACAAGTGGA
    TTAGCTGATCAACTACCAAGTAATTTATATATAGGAGAAGTGACTAAGGTTCAAAATGAT
    CAATACGGCTTAGCTAAAGAAGTTAGGGTTAAGACTGGTGCGGACTTAACAGATTTGAGT
    CATGTTTATGTTGCAAAAAGAGATCCTAAAACAATTCCTGATGATGAAAGCAGGGATAAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    843

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01759
  • symbol: SAOUHSC_01759
  • description: rod shape-determining protein MreC
  • length: 280
  • theoretical pI: 9.59859
  • theoretical MW: 31010
  • GRAVY: -0.398929

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan rod shape-determining protein MreC (TIGR00219; HMM-score: 147)
    and 4 more
    Genetic information processing Mobile and extrachromosomal element functions Plasmid functions integrating conjugative element protein, PFL_4705 family (TIGR03752; HMM-score: 18.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking preprotein translocase, YajC subunit (TIGR00739; HMM-score: 15)
    integrating conjugative element protein, PFL_4669 family (TIGR03761; HMM-score: 12.1)
    Cellular processes Cellular processes Cell division cell division protein FtsL (TIGR02209; HMM-score: 4.9)
  • TheSEED  :
    • Rod shape-determining protein MreC
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Bacterial Cytoskeleton  Rod shape-determining protein MreC
  • PFAM:
    no clan defined MreC; rod shape-determining protein MreC (PF04085; HMM-score: 135.4)
    and 12 more
    YajC; Preprotein translocase subunit (PF02699; HMM-score: 22.5)
    TSC22; TSC-22/dip/bun family (PF01166; HMM-score: 18.7)
    FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 17.4)
    no clan defined YabB; Initiation control protein YabA (PF06156; HMM-score: 16.6)
    IncA; IncA protein (PF04156; HMM-score: 15.4)
    OppC_N; N-terminal TM domain of oligopeptide transport permease C (PF12911; HMM-score: 15.2)
    DUF1845; Domain of unknown function (DUF1845) (PF08900; HMM-score: 15.1)
    bZIP (CL0018) bZIP_1; bZIP transcription factor (PF00170; HMM-score: 15)
    no clan defined Kinetocho_Slk19; Central kinetochore-associated (PF12709; HMM-score: 14.7)
    bZIP (CL0018) bZIP_2; Basic region leucine zipper (PF07716; HMM-score: 14.5)
    no clan defined Swi5; Swi5 (PF07061; HMM-score: 12.8)
    Shugoshin_N; Shugoshin N-terminal coiled-coil region (PF07558; HMM-score: 7.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 32 aa
    • SP(Sec/SPI): 0.795076
    • TAT(Tat/SPI): 0.001816
    • LIPO(Sec/SPII): 0.137729
    • Cleavage Site: CS pos: 32-33. SQS-QS. Pr: 0.5493
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSVSFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLKDISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSSQVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVTSGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDESRDK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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