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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1704 [new locus tag: SACOL_RS08690 ]
  • pan locus tag?: SAUPAN004255000
  • symbol: mreC
  • pan gene symbol?: mreC
  • synonym:
  • product: rod shape-determining protein MreC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1704 [new locus tag: SACOL_RS08690 ]
  • symbol: mreC
  • product: rod shape-determining protein MreC
  • replicon: chromosome
  • strand: -
  • coordinates: 1734158..1735000
  • length: 843
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGCTTAAGTTTTTTAAAAATAACAAATTAATTGTTGTTTTATGTGCAATTATCGTTTTT
    ATTGCATTAATTGGGCTGTCCATACGTTCACAATCTCAATCACCTCCTGAACAATATATA
    GGTGATTCTGTGTCTTTTGGACAACGAGTTGTGAGTTATCCAGTTAATTTTGTTGCTGGT
    ACGATTGGGGACTTTTTTAAAAAAGGAGATTCTAAAGAATCTAAAAATAAGATTAGCCAG
    TTAGAATCTAAGAACCAACAATTAGAAGCGGAAAATGAAAAATTAAAAAAAGAGCTTGAT
    TTAAAAGATATTTCAAAATTTGATCCTATTTCTACTACGGTTTTGGCAAGAAATCCGGAT
    CAGTGGATGAATACAATTGTAATTGATAAGGGATCTAAATCAGGTATAACTTCAAATATG
    GCTGTGATGACATCACAAGGTTTTGTTGGAAGAGTTACTAAAGTTAATAAATTTTCTTCA
    CAAGTTGATTTAATCTCAACTAATACACGTGCGGGTAAATTATCTGTAAATATACAACAC
    GGTTCTAAAAATATATTTGGTTTAATTGATCGTTATGATGAAAAGAACTCAGAACTTGTA
    ATTAGTGACATTAATAATAGAGATAATATCTCAAAAGGTGATAAAGTCGTTACAAGTGGA
    TTAGCTGATCAACTACCAAGTAATTTATATATAGGAGAAGTGACTAAGGTTCAAAATGAT
    CAATACGGCTTAGCTAAAGAAGTTAGGGTTAAGACTGGTGCGGACTTAACAGATTTGAGT
    CATGTTTATGTTGCAAAAAGAGATCCTAAAACAATTCCTGATGATGAAAGCAGGGATAAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    843

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1704 [new locus tag: SACOL_RS08690 ]
  • symbol: MreC
  • description: rod shape-determining protein MreC
  • length: 280
  • theoretical pI: 9.59859
  • theoretical MW: 31010
  • GRAVY: -0.398929

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan rod shape-determining protein MreC (TIGR00219; HMM-score: 147)
    and 4 more
    Genetic information processing Mobile and extrachromosomal element functions Plasmid functions integrating conjugative element protein, PFL_4705 family (TIGR03752; HMM-score: 18.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking preprotein translocase, YajC subunit (TIGR00739; HMM-score: 15)
    integrating conjugative element protein, PFL_4669 family (TIGR03761; HMM-score: 12.1)
    Cellular processes Cellular processes Cell division cell division protein FtsL (TIGR02209; HMM-score: 4.9)
  • TheSEED  :
    • Rod shape-determining protein MreC
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Bacterial Cytoskeleton  Rod shape-determining protein MreC
  • PFAM:
    no clan defined MreC; rod shape-determining protein MreC (PF04085; HMM-score: 135.4)
    and 12 more
    YajC; Preprotein translocase subunit (PF02699; HMM-score: 22.5)
    TSC22; TSC-22/dip/bun family (PF01166; HMM-score: 18.7)
    FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 17.4)
    no clan defined YabB; Initiation control protein YabA (PF06156; HMM-score: 16.6)
    IncA; IncA protein (PF04156; HMM-score: 15.4)
    OppC_N; N-terminal TM domain of oligopeptide transport permease C (PF12911; HMM-score: 15.2)
    DUF1845; Domain of unknown function (DUF1845) (PF08900; HMM-score: 15.1)
    bZIP (CL0018) bZIP_1; bZIP transcription factor (PF00170; HMM-score: 15)
    no clan defined Kinetocho_Slk19; Central kinetochore-associated (PF12709; HMM-score: 14.7)
    bZIP (CL0018) bZIP_2; Basic region leucine zipper (PF07716; HMM-score: 14.5)
    no clan defined Swi5; Swi5 (PF07061; HMM-score: 12.8)
    Shugoshin_N; Shugoshin N-terminal coiled-coil region (PF07558; HMM-score: 7.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 32 aa
    • SP(Sec/SPI): 0.795076
    • TAT(Tat/SPI): 0.001816
    • LIPO(Sec/SPII): 0.137729
    • Cleavage Site: CS pos: 32-33. SQS-QS. Pr: 0.5493
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSVSFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLKDISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSSQVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVTSGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDESRDK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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