PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1897 [new locus tag: NWMN_RS10915 ]
• pan locus tag^?: SAUPAN001370000
• symbol: NWMN_1897
• pan gene symbol^?: —
• synonym:
• product: phage Clp-like protease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1897 [new locus tag: NWMN_RS10915 ]
• symbol: NWMN_1897
• product: phage Clp-like protease
• replicon: chromosome
• strand: -
• coordinates: 2110562..2111299
• length: 738
• essential: unknown

⊟Accession numbers

• Gene ID: 5331161 NCBI
• RefSeq: YP_001332931 NCBI
• BioCyc:
• MicrobesOnline: 3707485 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAGCGGAGATTAAAGGCGTCATCGTTTCCAACGAAGATAAATGGGTTTACGAAATG
  CTTGGTATGGATTCGACTTGTCCTAAAGATGTTTTAACACAACTAGAATTTAGTGATGAA
  GATGTTGATATTATAATTAACTCAAATGGTGGTAACCTAGTAGCTGGTAGTGAAATATAT
  ACACATTTAAGAGCTCATAAAGGCAAAGTGAATGTTCGTATCACAGCAATAGCAGCAAGT
  GCGGCATCGCTTATCGCAATGGCTGGTGACCACATCGAAATGAGTCCGGTTGCTAGAATG
  ATGATTCACAATCCTTCAAGTATTGCGCAAGGAGAAGCGAAAGATCTAAATCATGCTGCA
  GAAACATTAGAACATGTTGGTCAAATAATGGCTGAGGCATATGCGGTTAGAGCTGGTAAA
  AACAAACAAGAACTTGTAGAAATGATGGCTAGGGAAACGTGGCTAAATGCTGATGAAGCC
  ATTGAACAAGGTTTTGCGGATAGTAAAATGTTTGAAAACGACAATATGCAAATTGTAGCA
  AGCAATACACAAGTGTTATCGAAAGATGTATTAAATCGTGTAACAGCTTTGGTAAGTAAA
  ACGCCAGAGGTTAACATTGATATTGACGCAATAGCAAATAAAGTAATTGAAAAAATAAAT
  ATGAAAGAAAAGGAATCAGAAATCGATGTTGCAGATAGTAAAGTATCAGCAAATGGATTT
  TCAAGATTCCTTTTTTAA
  60
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  738
  

⊟Protein

⊟General

• locus tag: NWMN_1897 [new locus tag: NWMN_RS10915 ]
• symbol: NWMN_1897
• description: phage Clp-like protease
• length: 245
• theoretical pI: 4.59748
• theoretical MW: 26839.4
• GRAVY: -0.161224

⊟Function

• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent Clp endopeptidase, proteolytic subunit ClpP (TIGR00493; EC 3.4.21.92; HMM-score: 63.1)
  and 1 more
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides signal peptide peptidase SppA, 36K type (TIGR00706; EC 3.4.-.-; HMM-score: 25.8)
• TheSEED:
• PFAM:
  ClpP_crotonase (CL0127) CLP_protease; Clp protease (PF00574; HMM-score: 110)
  and 5 more
  SDH_sah; Serine dehydrogenase proteinase (PF01972; HMM-score: 33.6)
  Peptidase_S49; Peptidase family S49 (PF01343; HMM-score: 15.1)
  no clan defined S-AdoMet_synt_N; S-adenosylmethionine synthetase, N-terminal domain (PF00438; HMM-score: 14.1)
  DUF3570; Protein of unknown function (DUF3570) (PF12094; HMM-score: 12.4)
  PLN_propep; Protealysin propeptide (PF16485; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008625
  • TAT(Tat/SPI): 0.000174
  • LIPO(Sec/SPII): 0.001238
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222109 NCBI
• RefSeq: YP_001332931 NCBI
• UniProt: A0A0H3K9K8 UniProt

⊟Protein sequence

• MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDIIINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMIHNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEAIEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEKINMKEKESEIDVADSKVSANGFSRFLF

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1890 < NWMN_1891 < NWMN_1892 < NWMN_1893 < NWMN_1894 < NWMN_1895 < NWMN_1896 < NWMN_1897 < NWMN_1898 < NWMN_1899 < NWMN_1900

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications