PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1899 [new locus tag: NWMN_RS10925 ]
• pan locus tag^?: SAUPAN001376000
• symbol: NWMN_1899
• pan gene symbol^?: —
• synonym:
• product: phage terminase large subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1899 [new locus tag: NWMN_RS10925 ]
• symbol: NWMN_1899
• product: phage terminase large subunit
• replicon: chromosome
• strand: -
• coordinates: 2112486..2114147
• length: 1662
• essential: unknown

⊟Accession numbers

• Gene ID: 5331163 NCBI
• RefSeq: YP_001332933 NCBI
• BioCyc:
• MicrobesOnline: 3707487 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGATTAGTAATAAATACGTTGATGAATATATAAATTTGTGGAAACAAGGAAAGATAATT
  TTAAATAAAGAAAGAATTGATCTCTTTAATTATCTACAAAAACATATATATTCACGAGAT
  GATGTATATTTTGATGAACAGAAAATCGAGGATTGTATCAAATTTATTGAAAAATGGTAT
  TTTCCAACATTACCATTTCAAAGGTTTATCATAGCTAATATATTTCTTATAGATAAAAAT
  ACAGATGAAGCTTTCTTTACAGAATTTGCTATTTTCATGGGACGTGGAGGCGGGAAAAAC
  GGTCTAATAAGTGCTATTAGTGATTTTCTTTCTACGCCCTTACACGGAGTTAAAGAATAT
  CACATCTCCATTGTTGCTAATAGTGAAGATCAAGCAAAAACATCGTTTGATGAAATCAGA
  ACCGTTTTAATGGATAACAAACGAAATAAGACGGGTAAAACGCCAAAAGCTCCTTATGAA
  GTTAGTAAAGCAAAAATAATAAACCGTGCAACTAAATCGGTTATTCGATATAACACATCA
  AACACAAAAACCAAAGACGGTGGACGTGAGGGGTGTGTTATTTTTGATGAAATTCATTAT
  TTCTTTGGTCCTGAAATGGTAAACGTCAAACGTGGTGGATTAGGTAAAAAGAAAAATAGA
  AGAACGTTTTATATAAGTACTGATGGTTTTGTTAGAGAGGGTTATATCGATGCAATGAAG
  CACAAAATTGCAAGTGTATTAAGTGGCAAGGTTAAAAATAGTAGATTGTTTGCTTTTTAT
  TGTAAGTTAGACGATCCAAAAGAAGTTGATGACAGACAGACGTGGGAAAAGGCGAACCCA
  ATGTTACATAAACCGTTATCAGAATACGCTAAAACACTGCTAAGTACGATTGAAGAAGAA
  TATAACGATTTACCATTCAACCGTTCAAATAAGCCCGAATTCATGACTAAGCGAATGAAT
  TTGCCTGAAGTTGACCTTGAAAAAGTAATAGCACCATGGAAAGAAATACTAGCGACTAAT
  AGAGAGATACCAAATTTAGATAATCAAATGTGTATTGGTGGTTTAGACTTTGCAAACATT
  CGAGATTTTGCAAGTGTAGGGCTATTATTCCGAAAAAACGATGATTACATTTGGTTAGGA
  CATTCGTTTGTAAGACAAGGGTTTTTGGATGATGTCAAATTAGAACCTCCTATTAAAGAA
  TGGGAAAAAATGGGATTATTGACCATTGTCGATGATGATGTCATTGAAATTGAATATATA
  GTTGATTGGTTTTTAAAGGCTAGAGAAAAATATGGGCTTGAAAAAGTCATAGCTGATAAT
  TATAGAACTGATATTGTAAGACGTGCGTTTGAGGATGCTGGCATAAAACTTGAAGTACTT
  AGAAATCCAAAAGCAATACATGGATTACTTGCACCACGTATCGATACAATGTTTGCGAAA
  CATAACGTAATATATGGAGACAATCCTTTGATGCGTTGGTTTACTAATAATGTTGCAGTA
  AAGGTTAAACCCGATGGTAATAAAGAATATATTAAAAAAGATGAAAATAGAAGAAAAACC
  GATGGGTTCATGGCTTTTGTTCACGCATTATATAGAGCAGACGATATAGTAGACAAAGAC
  ATGTCTAAAGCGCTTGATGCATTAATGAGTATAGATTTCTAA
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⊟Protein

⊟General

• locus tag: NWMN_1899 [new locus tag: NWMN_RS10925 ]
• symbol: NWMN_1899
• description: phage terminase large subunit
• length: 553
• theoretical pI: 8.46683
• theoretical MW: 64307.4
• GRAVY: -0.45859

⊟Function

• TIGRFAM:
• TheSEED:
• PFAM:
  P-loop_NTPase (CL0023) Terminase_1; Phage Terminase (PF03354; HMM-score: 143.3)
  and 1 more
  ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002105
  • TAT(Tat/SPI): 0.000095
  • LIPO(Sec/SPII): 0.000782
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222111 NCBI
• RefSeq: YP_001332933 NCBI
• UniProt: A0A0H3K9E3 UniProt

⊟Protein sequence

• MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYFDEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGLISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYEVSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKKNRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWEKANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWKEILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDVKLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAFEDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNKEYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1890 < NWMN_1891 < NWMN_1892 < NWMN_1893 < NWMN_1894 < NWMN_1895 < NWMN_1896 < NWMN_1897 < NWMN_1898 < NWMN_1899 < NWMN_1900

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications