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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_2050 [new locus tag: NWMN_RS11835 ]
- pan locus tag?: SAUPAN005444000
- symbol: NWMN_2050
- pan gene symbol?: czrB
- synonym:
- product: cation efflux family protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_2050 [new locus tag: NWMN_RS11835 ]
- symbol: NWMN_2050
- product: cation efflux family protein
- replicon: chromosome
- strand: +
- coordinates: 2266987..2267967
- length: 981
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5331249 NCBI
- RefSeq: YP_001333084 NCBI
- BioCyc:
- MicrobesOnline: 3707643 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGTCTCATTCACATCATCATCATGACCATATGCATAGTCATGTAACTACAAATAATAAG
AAAGTATTGTTTATATCGTTTTTAATAATCGGTCTATATATGTTTATCGAAATCATCGGC
GGTCTCCTTGCTAACAGCTTGGCATTACTATCTGACGGTATCCATATGTTTAGCGACACA
TTCTCATTAGGTGTTGCACTTGTCGCATTTATTTATGCTGAAAAGAATGCCACAACTACA
AAAACATTTGGTTATAAACGTTTCGAAGTACTCGCAGCGTTATTTAACGGTGTAACGCTT
TTTGTAATAAGTATTTTGATTGTTTTTGAAGCGATTAAACGTTTCTTTGTTCCTTCTGAA
GTTCAATCAAAAGAAATGTTAATCATTAGTATTATCGGTTTAATTGTCAATATCGTTGTT
GCATTCTTTATGTTTAAAGGCGGCGACACTTCACACAATTTAAATATGCGTGGTGCTTTT
CTACATGTTATCGGAGACTTATTAGGTTCAGTTGGCGCCATTACTGCAGCTATTTTAATT
TGGGCATTTGGATGGACAATCGCCGATCCTATCGCAAGTATTTTAGTTTCCGTTATTATT
TTAAAAAGTGCTTGGGGTATCACAAAATCTTCAATTAACATTTTAATGGAAGGCACACCA
AGTGATGTTGATATAGATGAAGTTATAACTACTATTAAAAAGGATTCACGAATACAAAGT
GTGCATGATTGCCATGTTTGGACAATTTCAAATGATATGAATGCATTGAGTTGTCATGTT
GTTGTAGACCATACATTGACAATGAAAGAATGTGAATTATTATTAGAAAACATTGAGCAT
GATTTATTACATTTAAATATTCACCATATGACTATTCAATTAGAAACGCCTAATCACAAA
CATGATGAATCGATTATATGTTCAGGAACACATAGTCATTCACATAACCATCATGCTCAT
CATCACGCGCATGTACATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_2050 [new locus tag: NWMN_RS11835 ]
- symbol: NWMN_2050
- description: cation efflux family protein
- length: 326
- theoretical pI: 6.75742
- theoretical MW: 36265.9
- GRAVY: 0.41411
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Cations and iron carrying compounds cation diffusion facilitator family transporter (TIGR01297; HMM-score: 329.2)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: no clan defined Cation_efflux; Cation efflux family (PF01545; HMM-score: 176.2)and 1 moreFtsK_4TM; 4TM region of DNA translocase FtsK/SpoIIIE (PF13491; HMM-score: 19.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 6
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.027321
- TAT(Tat/SPI): 0.000219
- LIPO(Sec/SPII): 0.000909
- predicted transmembrane helices (TMHMM): 6
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLANSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFVISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAFLHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEGTPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLENIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CzrA* (repression) regulon
CzrA* (TF) important in Zinc resistance; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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