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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2050 [new locus tag: NWMN_RS11835 ]
  • pan locus tag?: SAUPAN005444000
  • symbol: NWMN_2050
  • pan gene symbol?: czrB
  • synonym:
  • product: cation efflux family protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2050 [new locus tag: NWMN_RS11835 ]
  • symbol: NWMN_2050
  • product: cation efflux family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2266987..2267967
  • length: 981
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCTCATTCACATCATCATCATGACCATATGCATAGTCATGTAACTACAAATAATAAG
    AAAGTATTGTTTATATCGTTTTTAATAATCGGTCTATATATGTTTATCGAAATCATCGGC
    GGTCTCCTTGCTAACAGCTTGGCATTACTATCTGACGGTATCCATATGTTTAGCGACACA
    TTCTCATTAGGTGTTGCACTTGTCGCATTTATTTATGCTGAAAAGAATGCCACAACTACA
    AAAACATTTGGTTATAAACGTTTCGAAGTACTCGCAGCGTTATTTAACGGTGTAACGCTT
    TTTGTAATAAGTATTTTGATTGTTTTTGAAGCGATTAAACGTTTCTTTGTTCCTTCTGAA
    GTTCAATCAAAAGAAATGTTAATCATTAGTATTATCGGTTTAATTGTCAATATCGTTGTT
    GCATTCTTTATGTTTAAAGGCGGCGACACTTCACACAATTTAAATATGCGTGGTGCTTTT
    CTACATGTTATCGGAGACTTATTAGGTTCAGTTGGCGCCATTACTGCAGCTATTTTAATT
    TGGGCATTTGGATGGACAATCGCCGATCCTATCGCAAGTATTTTAGTTTCCGTTATTATT
    TTAAAAAGTGCTTGGGGTATCACAAAATCTTCAATTAACATTTTAATGGAAGGCACACCA
    AGTGATGTTGATATAGATGAAGTTATAACTACTATTAAAAAGGATTCACGAATACAAAGT
    GTGCATGATTGCCATGTTTGGACAATTTCAAATGATATGAATGCATTGAGTTGTCATGTT
    GTTGTAGACCATACATTGACAATGAAAGAATGTGAATTATTATTAGAAAACATTGAGCAT
    GATTTATTACATTTAAATATTCACCATATGACTATTCAATTAGAAACGCCTAATCACAAA
    CATGATGAATCGATTATATGTTCAGGAACACATAGTCATTCACATAACCATCATGCTCAT
    CATCACGCGCATGTACATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    981

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_2050 [new locus tag: NWMN_RS11835 ]
  • symbol: NWMN_2050
  • description: cation efflux family protein
  • length: 326
  • theoretical pI: 6.75742
  • theoretical MW: 36265.9
  • GRAVY: 0.41411

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds cation diffusion facilitator family transporter (TIGR01297; HMM-score: 329.2)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined Cation_efflux; Cation efflux family (PF01545; HMM-score: 176.2)
    and 1 more
    FtsK_4TM; 4TM region of DNA translocase FtsK/SpoIIIE (PF13491; HMM-score: 19.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.027321
    • TAT(Tat/SPI): 0.000219
    • LIPO(Sec/SPII): 0.000909
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLANSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFVISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAFLHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEGTPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLENIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: CzrA* (repression) regulon
    CzrA*(TF)important in Zinc resistance; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]