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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2138 [new locus tag: SACOL_RS11190 ]
  • pan locus tag?: SAUPAN005444000
  • symbol: SACOL2138
  • pan gene symbol?: czrB
  • synonym:
  • product: cation efflux family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2138 [new locus tag: SACOL_RS11190 ]
  • symbol: SACOL2138
  • product: cation efflux family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2198604..2199584
  • length: 981
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCTCATTCACATCATCATCATGACCATATGCATAGTCATGTAACTACAAATAATAAG
    AAAGTATTGTTTATATCGTTTTTAATAATCGGTCTATATATGTTTATCGAAATCATCGGC
    GGTCTCCTTGCTAACAGCTTGGCATTACTATCTGACGGTATCCATATGTTTAGCGACACA
    TTCTCATTAGGTGTTGCACTTGTCGCATTTATTTATGCTGAAAAGAATGCCACAACTACA
    AAAACATTTGGTTATAAACGTTTCGAAGTACTCGCAGCGTTATTTAACGGTGTAACGCTT
    TTTGTAATAAGTATTTTGATTGTTTTTGAAGCGATTAAACGTTTCTTTGTTCCTTCTGAA
    GTTCAATCAAAAGAAATGTTAATCATTAGTATTATCGGTTTAATTGTCAATATCGTTGTT
    GCATTCTTTATGTTTAAAGGCGGCGACACTTCACACAATTTAAATATGCGTGGTGCTTTT
    CTACATGTTATCGGAGACTTATTAGGTTCAGTTGGCGCCATTACTGCAGCTATTTTAATT
    TGGGCATTTGGATGGACAATCGCCGATCCTATCGCAAGTATTTTAGTTTCCGTTATTATT
    TTAAAAAGTGCTTGGGGTATCACAAAATCTTCAATTAACATTTTAATGGAAGGCACACCA
    AGTGATGTTGATATAGATGAAGTTATAACTACTATTAAAAAGGATTCACGAATACAAAGT
    GTGCATGATTGCCATGTTTGGACAATTTCAAATGATATGAATGCATTGAGTTGTCATGTT
    GTTGTAGACCATACATTGACAATGAAAGAATGTGAATTATTATTAGAAAACATTGAGCAT
    GATTTATTACATTTAAATATTCACCATATGACTATTCAATTAGAAACGCCTAATCACAAA
    CATGATGAATCGATTATATGTTCAGGAACACATAGTCATTCACATAACCATCATGCTCAT
    CATCACGCGCATGTACATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    981

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2138 [new locus tag: SACOL_RS11190 ]
  • symbol: SACOL2138
  • description: cation efflux family protein
  • length: 326
  • theoretical pI: 6.75742
  • theoretical MW: 36265.9
  • GRAVY: 0.41411

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds cation diffusion facilitator family transporter (TIGR01297; HMM-score: 329.2)
  • TheSEED  :
    • Zn(II) and Co(II) transmembrane diffusion facilitator
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Cobalt-zinc-cadmium resistance  Zn(II) and Co(II) transmembrane diffusion facilitator
  • PFAM:
    no clan defined Cation_efflux; Cation efflux family (PF01545; HMM-score: 176.2)
    and 1 more
    FtsK_4TM; 4TM region of DNA translocase FtsK/SpoIIIE (PF13491; HMM-score: 19.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.027321
    • TAT(Tat/SPI): 0.000219
    • LIPO(Sec/SPII): 0.000909
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLANSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFVISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAFLHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEGTPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLENIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CzrA (repression) regulon
    CzrA(TF)important in Zinc resistance; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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