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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1948 [new locus tag: SA_RS11185 ]
  • pan locus tag?: SAUPAN005444000
  • symbol: czrB
  • pan gene symbol?: czrB
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1948 [new locus tag: SA_RS11185 ]
  • symbol: czrB
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2197065..2198042
  • length: 978
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCTCATTCACATCATCATGACCATATGCATAGTCATGTAACTACAAATAATAAGAAA
    GTATTGTTTATATCGTTTTTAATAATCGGTCTATATATGTTTATCGAAATCATCGGCGGT
    CTCCTTGCTAACAGCTTGGCATTACTATCTGACGGTATCCATATGTTTAGCGACACATTC
    TCATTAGGTGTTGCACTTGTCGCATTTATTTATGCTGAAAAGAATGCCACAACTACAAAA
    ACATTTGGTTATAAACGTTTCGAAGTACTCGCAGCGTTATTTAACGGTGTAACGCTTTTT
    GTAATAAGTATTTTGATTGTTTTTGAAGCGATTAAACGTTTCTTTGTTCCTTCTGAAGTT
    CAATCAAAAGAAATGTTAATCATTAGTATTATCGGTTTAATTGTCAATATCGTTGTTGCA
    TTCTTTATGTTTAAAGGCGGCGACACTTCACACAATTTAAATATGCGTGGTGCTTTTCTA
    CATGTTATCGGAGACTTATTAGGTTCAGTTGGCGCCATTACTGCAGCTATTTTAATTTGG
    GCATTTGGATGGACAATCGCCGATCCTATCGCAAGTATTTTAGTTTCCGTTATTATTTTA
    AAAAGTGCTTGGGGTATCACAAAATCTTCAATTAACATTTTAATGGAAGGCACACCAAGT
    GATGTTGATATAGATGAAGTTATAACTACTATTAAAAAGGATTCACGAATACAAAGTGTG
    CATGATTGCCATGTTTGGACAATTTCAAATGATATGAATGCATTGAGTTGTCATGTTGTT
    GTAGACCATACATTGACAATGAAAGAATGTGAATTATTATTAGAAAACATTGAGCATGAT
    TTATTACATTTAAATATTCACCATATGACTATTCAATTAGAAACGCCTAATCACAAACAT
    GATGAATCGATTATATGTTCAGGAACACATAGTCATTCACATAACCATCATGCTCATCAT
    CACGCGCATGTACATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    978

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1948 [new locus tag: SA_RS11185 ]
  • symbol: CzrB
  • description: hypothetical protein
  • length: 325
  • theoretical pI: 6.73555
  • theoretical MW: 36128.7
  • GRAVY: 0.425231

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds cation diffusion facilitator family transporter (TIGR01297; HMM-score: 329.2)
  • TheSEED  :
    • Cadmium, cobalt and zinc/H(+)-K(+) antiporter CzcD
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Cobalt-zinc-cadmium resistance  Zn(II) and Co(II) transmembrane diffusion facilitator
  • PFAM:
    no clan defined Cation_efflux; Cation efflux family (PF01545; HMM-score: 176.2)
    and 2 more
    ZT_dimer; Dimerisation domain of Zinc Transporter (PF16916; HMM-score: 23.1)
    FtsK_4TM; 4TM region of DNA translocase FtsK/SpoIIIE (PF13491; HMM-score: 19.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.027348
    • TAT(Tat/SPI): 0.000197
    • LIPO(Sec/SPII): 0.000988
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSHSHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLANSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFVISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAFLHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEGTPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLENIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CzrA (repression) regulon
    CzrA(TF)important in Zinc resistance; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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