PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2215 [new locus tag: NWMN_RS12775 ]
• pan locus tag^?: SAUPAN005792000
• symbol: NWMN_2215
• pan gene symbol^?: spdB
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2215 [new locus tag: NWMN_RS12775 ]
• symbol: NWMN_2215
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2436664..2437401
• length: 738
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331339 NCBI
• RefSeq: YP_001333249 NCBI
• BioCyc:
• MicrobesOnline: 3707817 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  721
  
  GTGGAAAATGAAAAAAAGAAATACACGTTTAAAGATATAGCATGGAGAGATTTGCTTATA
  ATTCCAATTATAGGTGTTATATTTTATGCACTCACAATTGCAGCTTACATAGGACCAATT
  TTGTTTGGTTATTATGAAATCGATAGTAATCTGATATTTGTAGCGACTATCACACAAGCT
  TTAGCATATACGATTGGGATTGGCGCATTTTATTTATTTCATTTAAAAGTGATGCCATCA
  AGGCTTCGAGCTGGTTTTGCGTATATCAAAAAATATTGGCTTAGATTGTCAATAACGTAT
  ATTATTGCTCTCATCTTAATTTATACATATGAATATATGACACAGTTTCTACCTAAACAT
  TTGCAATATAGTGAAACAGCCAATGAACTTGAGCTAAATAAAATGTTTGAAGTGCCTGCA
  TTTTTACCTGTAGCATTCTTATTAATTGTGATTGTAGGTCCAATAGTTGAAGAAATCGTA
  TTTAGACATATTTTAATTGGAGAATTAGGTAAGAAGTTTAATTTTATAGCTATGAGTATT
  GTTTCGGTGTTCTTATTTGCATTTATTCATGTGACTGATGCAAAGTCGCCGTTTGAGTTT
  GGTCCATATCTCATACTATCCATCATATTAGTTTTCACTTATTTAAAATCTGGAAGAAAC
  CTAGGTTCAACAATTGCATTACATATAGCAAATAATTTTGTTTCTTTCGTTATATCAGTA
  ATAAGTATTTATGCGTAG
  60
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  738
  

⊟Protein

⊟General

• locus tag: NWMN_2215 [new locus tag: NWMN_RS12775 ]
• symbol: NWMN_2215
• description: hypothetical protein
• length: 245
• theoretical pI: 9.19765
• theoretical MW: 28034.3
• GRAVY: 0.82

⊟Function

• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Peptidase_U (CL0472) Abi; CAAX protease self-immunity (PF02517; HMM-score: 52.5)
  and 1 more
  no clan defined DUF2798; Protein of unknown function (DUF2798) (PF11391; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 7
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009267
  • TAT(Tat/SPI): 0.000163
  • LIPO(Sec/SPII): 0.002173
• predicted transmembrane helices (TMHMM): 7

⊟Accession numbers

• GI: 151222427 NCBI
• RefSeq: YP_001333249 NCBI
• UniProt: A0A0H3KA33 UniProt

⊟Protein sequence

• MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGYYEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYIIALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIVFRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSGRNLGSTIALHIANNFVSFVISVISIYA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Matthew B Frankel, Brandon M Wojcik, Andrea C DeDent, Dominique M Missiakas, Olaf Schneewind
ABI domain-containing proteins contribute to surface protein display and cell division in Staphylococcus aureus.
Mol Microbiol: 2010, 78(1);238-52
[PubMed:20923422] [WorldCat.org] [DOI] (I p)