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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_2215 [new locus tag: NWMN_RS12775 ]
- pan locus tag?: SAUPAN005792000
- symbol: NWMN_2215
- pan gene symbol?: spdB
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_2215 [new locus tag: NWMN_RS12775 ]
- symbol: NWMN_2215
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 2436664..2437401
- length: 738
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5331339 NCBI
- RefSeq: YP_001333249 NCBI
- BioCyc:
- MicrobesOnline: 3707817 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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721GTGGAAAATGAAAAAAAGAAATACACGTTTAAAGATATAGCATGGAGAGATTTGCTTATA
ATTCCAATTATAGGTGTTATATTTTATGCACTCACAATTGCAGCTTACATAGGACCAATT
TTGTTTGGTTATTATGAAATCGATAGTAATCTGATATTTGTAGCGACTATCACACAAGCT
TTAGCATATACGATTGGGATTGGCGCATTTTATTTATTTCATTTAAAAGTGATGCCATCA
AGGCTTCGAGCTGGTTTTGCGTATATCAAAAAATATTGGCTTAGATTGTCAATAACGTAT
ATTATTGCTCTCATCTTAATTTATACATATGAATATATGACACAGTTTCTACCTAAACAT
TTGCAATATAGTGAAACAGCCAATGAACTTGAGCTAAATAAAATGTTTGAAGTGCCTGCA
TTTTTACCTGTAGCATTCTTATTAATTGTGATTGTAGGTCCAATAGTTGAAGAAATCGTA
TTTAGACATATTTTAATTGGAGAATTAGGTAAGAAGTTTAATTTTATAGCTATGAGTATT
GTTTCGGTGTTCTTATTTGCATTTATTCATGTGACTGATGCAAAGTCGCCGTTTGAGTTT
GGTCCATATCTCATACTATCCATCATATTAGTTTTCACTTATTTAAAATCTGGAAGAAAC
CTAGGTTCAACAATTGCATTACATATAGCAAATAATTTTGTTTCTTTCGTTATATCAGTA
ATAAGTATTTATGCGTAG60
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738
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_2215 [new locus tag: NWMN_RS12775 ]
- symbol: NWMN_2215
- description: hypothetical protein
- length: 245
- theoretical pI: 9.19765
- theoretical MW: 28034.3
- GRAVY: 0.82
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: Peptidase_U (CL0472) Abi; CAAX protease self-immunity (PF02517; HMM-score: 52.5)and 1 moreno clan defined DUF2798; Protein of unknown function (DUF2798) (PF11391; HMM-score: 12.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 7
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.009267
- TAT(Tat/SPI): 0.000163
- LIPO(Sec/SPII): 0.002173
- predicted transmembrane helices (TMHMM): 7
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGYYEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYIIALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIVFRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSGRNLGSTIALHIANNFVSFVISVISIYA
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Matthew B Frankel, Brandon M Wojcik, Andrea C DeDent, Dominique M Missiakas, Olaf Schneewind
ABI domain-containing proteins contribute to surface protein display and cell division in Staphylococcus aureus.
Mol Microbiol: 2010, 78(1);238-52
[PubMed:20923422] [WorldCat.org] [DOI] (I p)