PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS00790 [old locus tag: NWMN_0144 ]
• pan locus tag^?: SAUPAN001057000
• symbol: NWMN_RS00790
• pan gene symbol^?: gisB
• synonym:
• product: ABC transporter permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS00790 [old locus tag: NWMN_0144 ]
• symbol: NWMN_RS00790
• product: ABC transporter permease
• replicon: chromosome
• strand: +
• coordinates: 181928..183406
• length: 1479
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (181928..183406) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0144

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGAATTGTACATTCAGACTGTTGACTTCTAATAAATTTGACATGATGTTGTGCCTCCCT
  ATTCACAAATGTGTAAATTTATTCTATACTTTTGTATATCATAGTAATCTACTCAAGATT
  ATTTTACAAGGAGTGATTATCATAATTACTTATTTAGAATCTTTTAAGTCGTTGTATCAC
  AAGGCATTTTACAAGTTTGTATTATCGGTATTAAGCTTGCCAATATTTTTATATGCAGTG
  ATAAAGTTTTTCTTTTCTGCTAAGAGGAAAAATTTTTACGCTAATAATTCTGAAATTTCA
  GAAATTGAACAGGCGTTACATCAAAAATATAAATATTTATCGCAGCAAAAGTCATCCACA
  CAAATACATAAAGAAGCATTAAAAATATTCAAGGCACAAAGTTCTAATACGAGTTCAAAG
  AATATTGAACAAGCACATTTTTCAACATACTTTGAAAATGTATTATTTCATAAGTTCATC
  ATGATCAAAGTGATATTGGCCTTGCCGATGTTCATCTTATTGACTTTTTATTTACAGCCA
  TTAGTTAGATATATTTTTGAACGAATTGTCATGGCTGTGATTGTCATCATTGGTGTTATT
  GTCAGTGTGTTTACCATTCTGTATTTTTCACCGCTTGATGCGGCTTATAGCATACTGGGA
  CAAAATGCAACAAAGGCACAGATACATCAATTCAATGTATTACATCATCTTAACGAACCT
  TATTTTATTCAATTGTGGGATACCATCAAGGGTGTTTTTACCTTTGACTTAGGTACGACT
  TACAAAGGGAATGAGGTTGTGACTAAAGCAGTTGGCGAAAGAATTCCAATTACAATAATT
  GTCGCAGTATTAGCGCTAATGGTGGCATTAATTATTGCAATACCAATTGGTATTATCAGT
  GCGATGAAGCGAAATAGTTGGCTTGATATCACGTTAATGATAATTGCATTAATTGGTTTA
  TCTATTCCAAGTTTCTGGCAAGGGCTATTATTCATTTTAGCGTTCTCATTGAAATTGGAT
  ATTTTGCCACCATCTTATATGCCAGAACATCCAATATCGTTGATTTTACCTGTACTTGTC
  ATTGGAACAAGTATTGCTGCTTCTATCACGCGTATGACAAGGTCTTCTGTACTTGAAGTA
  ATGCGCAGCGATTATGTTTTAACTGCTTATGCAAAAGGATTATCGACGACACAAGTTGTT
  ATTAAACATATTTTGAAAAATGCCATTATTCCAATTGTAACGTTAGTTGGTCTTCTAGTG
  GCAGAGTTACTAGGCGGTTCAGCAGTGACGGAACAAGTATTTAACATTAATGGTATCGGG
  CGTTATATCGTCCAAAAACAACTAATACCTGATATTCCAGCAGTCATGGGTGGGGTCGTA
  TATATATCAATTGTAATATCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATC
  GATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGA
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⊟Protein

⊟General

• locus tag: NWMN_RS00790 [old locus tag: NWMN_0144 ]
• symbol: NWMN_RS00790
• description: ABC transporter permease
• length: 492
• theoretical pI: 9.86158
• theoretical MW: 55575.1
• GRAVY: 0.681301

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 205.4)
  and 1 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 35.6)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 139)
  and 4 more
  Transporter (CL0375) Fig1; Ca2+ regulator and membrane fusion protein Fig1 (PF12351; HMM-score: 18.2)
  no clan defined Orbi_VP2; Orbivirus outer capsid protein VP2 (PF00898; HMM-score: 10.1)
  DUF3995; Protein of unknown function (DUF3995) (PF13160; HMM-score: 7.7)
  COX6C; Cytochrome c oxidase subunit VIc (PF02937; HMM-score: 6.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 8
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002009
  • TAT(Tat/SPI): 0.000094
  • LIPO(Sec/SPII): 0.01793
• predicted transmembrane helices (TMHMM): 9

⊟Accession numbers

• GI: see NWMN_0144
• RefSeq: WP_001797475 NCBI
• UniProt: see NWMN_0144

⊟Protein sequence

• MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQGVIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEIEQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFIMIKVILALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSILGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIPITIIVAVLALMVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILAFSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAKGLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIPDIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications