PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS04770 [old locus tag: NWMN_0847 ]
• pan locus tag^?: SAUPAN003116000
• symbol: NWMN_RS04770
• pan gene symbol^?: —
• synonym:
• product: 2-isopropylmalate synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS04770 [old locus tag: NWMN_0847 ]
• symbol: NWMN_RS04770
• product: 2-isopropylmalate synthase
• replicon: chromosome
• strand: +
• coordinates: 943067..944212
• length: 1146
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (943067..944212) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0847

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGATTCGAATTCAAGATAATACAATAAGGGATGGTATGCAACAAAGTAATGTTCGAAAA
  AGTCTAATTATAAAAAAAGAAGTATTGAAACAAATTAACAAGTTAAATATAAATTCTGTT
  GAAGTAGGCATGTGTACAACTATCGAGGATGAATTTAATATTCATCAATTCAGAGACATT
  TTAAGTCCTGAAAAAGAATTAGTAGTATTGACTAGGCTTAATGAAAAAGAAATAAAAAAA
  ATAGTCAAATTGAAAATTCATAATTTAGTGGTAAAAATACTATTGCCAATATCTGACTTG
  CATATAAAAGAAAAGCTTAATTTTTCAAATAAATATTATATTCAGAAAATCAAAGACTGC
  TTGGATATATTAAAGAAAGATAAAAAAGGAGTAGATATTTGTTTTGAAGATGGAACAAGG
  ACTTCTAGAGAAAAATTGAAAGAATACATGGAAATTATTTCAAAATATCAAGTTAGAACA
  GTTACATTTGCGGACACTGTAGGATGTTCGACACCATTAGAATACGGAGATATTTTTAAT
  TACTTTGTAAAAAAATATTCTAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGG
  TTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACA
  TTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTG
  ACAAAAAAACAAATAGAAAGTACGGAATTCACTTTACCCGACGTATATAAAACTAGTATT
  AATATTTCTAAAATTCTCGATTTTCAAATATCAGAAAACAAACCTATAATTGGTGAAAAT
  ATATTTAAACATGAATCAGGAATTCATCAAGATGGTACTAAAAAAAATATAAATATGTAT
  CAATATTTAGTTCCTAGTGATTTAGGATTGAAAAATTCACAAGTTGTTCAGTTTCCAATA
  AGTAATATTTCTAGTAAGAAAATCTTGCACAATAAATTTAAATCAATAGTTAACACTGAA
  GAAATTGATGAAAATATTTCTTTCTATAAACTTGTGAACAAGTTTTCACCTGAAGTAGCA
  CCTGAAGATACAGTGGATTTACTTCAGATAATAAAAAGGAGGAGTAAAGATGGAAATTTT
  AAATGA
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⊟Protein

⊟General

• locus tag: NWMN_RS04770 [old locus tag: NWMN_0847 ]
• symbol: NWMN_RS04770
• description: 2-isopropylmalate synthase
• length: 381
• theoretical pI: 9.15783
• theoretical MW: 43674.3
• GRAVY: -0.309974

⊟Function

• TIGRFAM:
  Metabolism Amino acid biosynthesis Pyruvate family 2-isopropylmalate synthase (TIGR00973; EC 2.3.3.13; HMM-score: 227.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 222.4)
  Metabolism Central intermediary metabolism Nitrogen fixation homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 222.4)
  isopropylmalate/citramalate/homocitrate synthases (TIGR02090; HMM-score: 201.7)
  and 6 more
  homocitrate synthase (TIGR02146; EC 2.3.3.14; HMM-score: 173.4)
  Unknown function General citramalate synthase (TIGR00977; EC 2.3.1.182; HMM-score: 109)
  Metabolism Energy metabolism Other 4-hydroxy-2-oxovalerate aldolase (TIGR03217; EC 4.1.3.39; HMM-score: 62.9)
  Metabolism Amino acid biosynthesis Pyruvate family 2-isopropylmalate synthase (TIGR00970; EC 2.3.3.13; HMM-score: 56.4)
  Metabolism Transport and binding proteins Cations and iron carrying compounds oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 26.7)
  Metabolism Energy metabolism Other oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 26.7)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  TIM_barrel (CL0036) HMGL-like; HMGL-like (PF00682; HMM-score: 189.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00458
  • TAT(Tat/SPI): 0.000213
  • LIPO(Sec/SPII): 0.000394
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446542019 NCBI
• RefSeq: WP_000619365 NCBI
• UniProt: see NWMN_0847

⊟Protein sequence

• MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGMCTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHIKEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDGTRTSREKLKEYMEIISKYQVRTVTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIETTFLGIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPIIGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFKSIVNTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications