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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00914
  • pan locus tag?: SAUPAN003116000
  • symbol: SAOUHSC_00914
  • pan gene symbol?: —
  • synonym:
  • product: 2-isopropylmalate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00914
  • symbol: SAOUHSC_00914
  • product: 2-isopropylmalate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 886283..887428
  • length: 1146
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGATTCGAATTCAAGATAATACAATAAGGGATGGTATGCAACAAAGTAATGTTCGAAAA
    AGTCTAATTATAAAAAAAGAAGTATTGAAACAAATTAACAAGTTAAATATAAATTCTGTT
    GAAGTAGGCATGTGTACAACTATCGAGGATGAATTTAATATTCATCAATTCAGAGACATT
    TTAAGTCCTGAAAAAGAATTAGTAGTATTGACTAGGCTTAATGAAAAAGAAATAAAAAAA
    ATAGTCAAATTGAAAATTCATAATTTAGTGGTAAAAATACTATTGCCAATATCTGACTTG
    CATATAAAAGAAAAGCTTAATTTTTCAAATAAATATTATATTCAGAAAATCAAAGACTGC
    TTGGATATATTAAAGAAAGATAAAAAAGGAGTAGATATTTGTTTTGAAGATGGAACAAGG
    ACTTCTAGAGAAAAATTGAAAGAATACATGGAAATTATTTCAAAATATCAAGTTAGAACA
    GTTACATTTGCGGACACTGTAGGATGTTCGACACCATTAGAATACGGAGATATTTTTAAT
    TACTTTGTAAAAAAATATTCTAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGG
    TTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACA
    TTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTG
    ACAAAAAAACAAATAGAAAGTACGGAATTCACTTTACCCGACGTATATAAAACTAGTATT
    AATATTTCTAAAATTCTCGATTTTCAAATATCAGAAAACAAACCTATAATTGGTGAAAAT
    ATATTTAAACATGAATCAGGAATTCATCAAGATGGTACTAAAAAAAATATAAATATGTAT
    CAATATTTAGTTCCTAGTGATTTAGGATTGAAAAATTCACAAGTTGTTCAGTTTCCAATA
    AGTAATATTTCTAGTAAGAAAATCTTGCACAATAAATTTAAATCAATAGTTAACACTGAA
    GAAATTGATGAAAATATTTCTTTCTATAAACTTGTGAACAAGTTTTCACCTGAAGTAGCA
    CCTGAAGATACAGTGGATTTACTTCAGATAATAAAAAGGAGGAGTAAAGATGGAAATTTT
    AAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1146

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00914
  • symbol: SAOUHSC_00914
  • description: 2-isopropylmalate synthase
  • length: 381
  • theoretical pI: 9.15783
  • theoretical MW: 43674.3
  • GRAVY: -0.309974

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.3.13?  ExPASy
    2-isopropylmalate synthase Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family 2-isopropylmalate synthase (TIGR00973; EC 2.3.3.13; HMM-score: 227.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 222.4)
    Metabolism Central intermediary metabolism Nitrogen fixation homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 222.4)
    isopropylmalate/citramalate/homocitrate synthases (TIGR02090; HMM-score: 201.7)
    and 6 more
    homocitrate synthase (TIGR02146; EC 2.3.3.14; HMM-score: 173.4)
    Unknown function General citramalate synthase (TIGR00977; EC 2.3.1.182; HMM-score: 109)
    Metabolism Energy metabolism Other 4-hydroxy-2-oxovalerate aldolase (TIGR03217; EC 4.1.3.39; HMM-score: 62.9)
    Metabolism Amino acid biosynthesis Pyruvate family 2-isopropylmalate synthase (TIGR00970; EC 2.3.3.13; HMM-score: 56.4)
    Metabolism Transport and binding proteins Cations and iron carrying compounds oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 26.7)
    Metabolism Energy metabolism Other oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 26.7)
  • TheSEED  :
    • FIG01108309: hypothetical protein
  • PFAM:
    TIM_barrel (CL0036) HMGL-like; HMGL-like (PF00682; HMM-score: 189.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00458
    • TAT(Tat/SPI): 0.000213
    • LIPO(Sec/SPII): 0.000394
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGMCTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHIKEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDGTRTSREKLKEYMEIISKYQVRTVTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIETTFLGIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPIIGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFKSIVNTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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